Desulfotomaculum copahuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B7LH55|A0A1B7LH55_9FIRM Putative heme d1 biosynthesis radical SAM protein NirJ1 OS=Desulfotomaculum copahuensis OX=1838280 GN=A6M21_06295 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84VTVDD6 pKa = 2.94QDD8 pKa = 3.66LCISCGSCVDD18 pKa = 3.65TCPEE22 pKa = 3.78VFDD25 pKa = 4.67WNGDD29 pKa = 3.59DD30 pKa = 3.6KK31 pKa = 11.7AQAIVDD37 pKa = 4.07EE38 pKa = 4.76VPGEE42 pKa = 4.43LEE44 pKa = 4.28DD45 pKa = 3.84QAHH48 pKa = 5.86EE49 pKa = 4.06AVEE52 pKa = 4.14NCPVNAIAEE61 pKa = 4.46KK62 pKa = 10.75

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B7LBI9|A0A1B7LBI9_9FIRM Transcriptional regulator OS=Desulfotomaculum copahuensis OX=1838280 GN=A6M21_15360 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.44QPKK8 pKa = 9.61ARR10 pKa = 11.84KK11 pKa = 7.83HH12 pKa = 5.56KK13 pKa = 10.34KK14 pKa = 7.27MHH16 pKa = 6.12GFLKK20 pKa = 10.58RR21 pKa = 11.84MASKK25 pKa = 10.69AGRR28 pKa = 11.84NVIKK32 pKa = 10.47RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3325

0

3325

993877

28

2854

298.9

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.469 ± 0.057

1.278 ± 0.021

4.679 ± 0.031

6.18 ± 0.041

3.607 ± 0.026

8.897 ± 0.039

1.895 ± 0.018

5.519 ± 0.04

4.248 ± 0.036

10.669 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.019

3.196 ± 0.027

5.034 ± 0.029

3.433 ± 0.03

6.851 ± 0.042

4.616 ± 0.028

4.925 ± 0.029

7.989 ± 0.035

1.079 ± 0.017

2.886 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski