Cyanophage NATL2A-133

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Tangaroavirus; Prochlorococcus virus NATL2A133

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SP45|E3SP45_9CAUD Predicted protein OS=Cyanophage NATL2A-133 OX=445692 GN=CYPG_00022 PE=4 SV=1
MM1 pKa = 6.07TTSYY5 pKa = 10.5IPPLPPVSQVEE16 pKa = 4.26QPDD19 pKa = 4.02TEE21 pKa = 4.17EE22 pKa = 4.53LQTSLGIQEE31 pKa = 4.66SGGNYY36 pKa = 7.14QARR39 pKa = 11.84NDD41 pKa = 3.87DD42 pKa = 3.88AYY44 pKa = 10.86GPEE47 pKa = 3.93NPALGKK53 pKa = 10.31YY54 pKa = 7.53QLL56 pKa = 4.0

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SP81|E3SP81_9CAUD Predicted protein OS=Cyanophage NATL2A-133 OX=445692 GN=CYPG_00061 PE=4 SV=1
MM1 pKa = 6.74TTPTTRR7 pKa = 11.84KK8 pKa = 8.49PRR10 pKa = 11.84RR11 pKa = 11.84AKK13 pKa = 10.01RR14 pKa = 11.84AKK16 pKa = 10.57APVKK20 pKa = 10.42KK21 pKa = 9.87QVQLPVIPGNVRR33 pKa = 11.84PNVEE37 pKa = 4.8LISMQAYY44 pKa = 6.94WQDD47 pKa = 2.42IRR49 pKa = 11.84NRR51 pKa = 11.84SSIHH55 pKa = 5.7NYY57 pKa = 8.42EE58 pKa = 3.92VNEE61 pKa = 3.89AMNEE65 pKa = 3.57LRR67 pKa = 11.84IAVKK71 pKa = 8.41WTNARR76 pKa = 11.84IDD78 pKa = 3.78QLCDD82 pKa = 3.73RR83 pKa = 11.84IKK85 pKa = 10.83EE86 pKa = 4.18VAPP89 pKa = 4.05

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12497

40

1486

201.6

22.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.762 ± 0.729

0.776 ± 0.175

6.578 ± 0.299

5.849 ± 0.572

3.161 ± 0.241

7.594 ± 0.545

1.64 ± 0.125

5.745 ± 0.241

6.49 ± 0.594

7.49 ± 0.519

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.32

5.737 ± 0.55

3.977 ± 0.483

3.961 ± 0.354

4.001 ± 0.402

6.594 ± 0.577

7.482 ± 0.788

6.081 ± 0.259

1.32 ± 0.143

3.353 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski