Epulopiscium sp. SCG-C07WGA-EpuloA2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2MA43|A0A1V2MA43_9FIRM Pyridoxal phosphate homeostasis protein OS=Epulopiscium sp. SCG-C07WGA-EpuloA2 OX=1712378 GN=AN641_00675 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.44KK3 pKa = 10.03FLSVVLSSTMLIAFAGCTSSEE24 pKa = 4.56TNTSTDD30 pKa = 2.62TSNLVGVSMPTQDD43 pKa = 3.63LQRR46 pKa = 11.84WNQDD50 pKa = 3.07GEE52 pKa = 4.23NMKK55 pKa = 10.72AQLEE59 pKa = 4.46SAGYY63 pKa = 10.35DD64 pKa = 2.9VDD66 pKa = 4.09LQYY69 pKa = 11.57AANDD73 pKa = 3.1IATQVSQIEE82 pKa = 4.11NMISGGVKK90 pKa = 8.58TLVVASIDD98 pKa = 3.6GSALGTVMEE107 pKa = 4.26QAKK110 pKa = 10.84SNGIEE115 pKa = 3.99VIAYY119 pKa = 8.34DD120 pKa = 4.26RR121 pKa = 11.84LIMGTDD127 pKa = 3.94AISYY131 pKa = 10.43YY132 pKa = 9.8STFDD136 pKa = 3.08NWDD139 pKa = 3.33VGVKK143 pKa = 9.18QGEE146 pKa = 4.53YY147 pKa = 10.15IVDD150 pKa = 3.73KK151 pKa = 11.14LKK153 pKa = 10.95LDD155 pKa = 4.09STDD158 pKa = 2.91EE159 pKa = 4.3TYY161 pKa = 10.37TIEE164 pKa = 5.96FITGDD169 pKa = 3.84PGDD172 pKa = 3.73NNINFFFGGAMSILQPYY189 pKa = 9.5LDD191 pKa = 4.77EE192 pKa = 4.79GTLEE196 pKa = 4.22CLSGQTEE203 pKa = 4.12QTEE206 pKa = 4.47VATVNWATDD215 pKa = 3.25VAQARR220 pKa = 11.84FEE222 pKa = 4.84NILASYY228 pKa = 8.34YY229 pKa = 10.51TDD231 pKa = 3.29TDD233 pKa = 3.9LDD235 pKa = 3.62IVLASNDD242 pKa = 3.13STAQGVTNALVSSYY256 pKa = 11.38DD257 pKa = 3.12GAYY260 pKa = 9.81PLLTGQDD267 pKa = 3.59CDD269 pKa = 3.72VVSVQNIISGKK280 pKa = 8.2QEE282 pKa = 3.4MSIFKK287 pKa = 9.0DD288 pKa = 3.49TRR290 pKa = 11.84DD291 pKa = 3.43LAAKK295 pKa = 8.05TVEE298 pKa = 4.23MVDD301 pKa = 3.8ALMKK305 pKa = 10.69GSEE308 pKa = 4.32PPINDD313 pKa = 2.81NKK315 pKa = 10.8TYY317 pKa = 11.18DD318 pKa = 3.73NGTGIIPSYY327 pKa = 10.14LCEE330 pKa = 4.08PVVATLNNYY339 pKa = 7.54EE340 pKa = 4.28ALLIEE345 pKa = 4.33TGYY348 pKa = 8.74YY349 pKa = 8.05TAEE352 pKa = 4.04EE353 pKa = 4.28VQPP356 pKa = 4.05

Molecular weight: 38.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2MB14|A0A1V2MB14_9FIRM Coenzyme A biosynthesis bifunctional protein CoaBC OS=Epulopiscium sp. SCG-C07WGA-EpuloA2 OX=1712378 GN=coaBC PE=3 SV=1
MM1 pKa = 7.2KK2 pKa = 10.24RR3 pKa = 11.84VFAFPLYY10 pKa = 10.36LLSIQFSCHH19 pKa = 5.0SCPFQAATAKK29 pKa = 10.06VAEE32 pKa = 4.32LRR34 pKa = 11.84RR35 pKa = 11.84TIVNKK40 pKa = 7.05QTKK43 pKa = 8.5LHH45 pKa = 5.81PRR47 pKa = 11.84KK48 pKa = 10.11VFLGSPLRR56 pKa = 11.84IFWVGDD62 pKa = 3.56STEE65 pKa = 4.73LEE67 pKa = 4.25AKK69 pKa = 9.96SGRR72 pKa = 11.84SLLSQTGAGGLVGPGRR88 pKa = 11.84GGHH91 pKa = 4.36WRR93 pKa = 11.84GGKK96 pKa = 9.22VRR98 pKa = 11.84GRR100 pKa = 11.84RR101 pKa = 11.84GKK103 pKa = 9.25GTFAVQLAALCEE115 pKa = 4.15AGTVGRR121 pKa = 11.84YY122 pKa = 8.37RR123 pKa = 11.84AGEE126 pKa = 3.83EE127 pKa = 4.24SEE129 pKa = 4.16

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1923

0

1923

670132

37

5516

348.5

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.196 ± 0.068

1.061 ± 0.023

5.712 ± 0.063

7.165 ± 0.066

4.433 ± 0.053

6.069 ± 0.053

1.47 ± 0.023

10.135 ± 0.075

7.743 ± 0.064

8.962 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.032

6.344 ± 0.054

3.16 ± 0.033

3.197 ± 0.032

2.848 ± 0.038

5.949 ± 0.044

6.06 ± 0.101

6.092 ± 0.047

0.785 ± 0.017

4.136 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski