Amino acid dipepetide frequency for Nitrosomonas aestuarii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.668AlaAla: 8.668 ± 0.134	0.994AlaCys: 0.994 ± 0.034	4.563AlaAsp: 4.563 ± 0.067	5.254AlaGlu: 5.254 ± 0.087	3.38AlaPhe: 3.38 ± 0.055	6.296AlaGly: 6.296 ± 0.092	1.962AlaHis: 1.962 ± 0.041	6.165AlaIle: 6.165 ± 0.086	3.794AlaLys: 3.794 ± 0.06	9.405AlaLeu: 9.405 ± 0.105	2.284AlaMet: 2.284 ± 0.049	3.363AlaAsn: 3.363 ± 0.056	2.833AlaPro: 2.833 ± 0.051	3.618AlaGln: 3.618 ± 0.067	4.584AlaArg: 4.584 ± 0.068	4.989AlaSer: 4.989 ± 0.073	4.389AlaThr: 4.389 ± 0.072	5.981AlaVal: 5.981 ± 0.08	1.089AlaTrp: 1.089 ± 0.035	2.459AlaTyr: 2.459 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.873CysAla: 0.873 ± 0.034	0.181CysCys: 0.181 ± 0.015	0.555CysAsp: 0.555 ± 0.023	0.564CysGlu: 0.564 ± 0.023	0.477CysPhe: 0.477 ± 0.02	0.859CysGly: 0.859 ± 0.025	0.354CysHis: 0.354 ± 0.022	0.663CysIle: 0.663 ± 0.028	0.429CysLys: 0.429 ± 0.022	0.994CysLeu: 0.994 ± 0.03	0.23CysMet: 0.23 ± 0.015	0.433CysAsn: 0.433 ± 0.02	0.478CysPro: 0.478 ± 0.021	0.37CysGln: 0.37 ± 0.02	0.574CysArg: 0.574 ± 0.019	0.607CysSer: 0.607 ± 0.024	0.519CysThr: 0.519 ± 0.024	0.66CysVal: 0.66 ± 0.021	0.137CysTrp: 0.137 ± 0.01	0.341CysTyr: 0.341 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.678AspAla: 4.678 ± 0.074	0.541AspCys: 0.541 ± 0.026	2.988AspAsp: 2.988 ± 0.061	3.638AspGlu: 3.638 ± 0.059	2.479AspPhe: 2.479 ± 0.046	3.659AspGly: 3.659 ± 0.067	1.258AspHis: 1.258 ± 0.033	3.87AspIle: 3.87 ± 0.067	2.804AspLys: 2.804 ± 0.045	5.246AspLeu: 5.246 ± 0.073	1.416AspMet: 1.416 ± 0.037	2.415AspAsn: 2.415 ± 0.052	2.372AspPro: 2.372 ± 0.044	2.205AspGln: 2.205 ± 0.048	2.681AspArg: 2.681 ± 0.053	3.14AspSer: 3.14 ± 0.046	2.91AspThr: 2.91 ± 0.054	3.284AspVal: 3.284 ± 0.055	0.867AspTrp: 0.867 ± 0.023	1.994AspTyr: 1.994 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.17GluAla: 5.17 ± 0.083	0.496GluCys: 0.496 ± 0.022	2.651GluAsp: 2.651 ± 0.05	3.332GluGlu: 3.332 ± 0.069	2.254GluPhe: 2.254 ± 0.053	3.073GluGly: 3.073 ± 0.053	1.487GluHis: 1.487 ± 0.038	4.85GluIle: 4.85 ± 0.077	4.008GluLys: 4.008 ± 0.067	6.06GluLeu: 6.06 ± 0.077	1.648GluMet: 1.648 ± 0.037	2.903GluAsn: 2.903 ± 0.054	1.968GluPro: 1.968 ± 0.049	3.151GluGln: 3.151 ± 0.066	3.479GluArg: 3.479 ± 0.057	3.721GluSer: 3.721 ± 0.057	3.569GluThr: 3.569 ± 0.058	3.504GluVal: 3.504 ± 0.061	0.758GluTrp: 0.758 ± 0.027	1.645GluTyr: 1.645 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.345PheAla: 3.345 ± 0.052	0.539PheCys: 0.539 ± 0.021	2.719PheAsp: 2.719 ± 0.054	2.504PheGlu: 2.504 ± 0.046	2.063PhePhe: 2.063 ± 0.051	2.911PheGly: 2.911 ± 0.058	0.923PheHis: 0.923 ± 0.031	2.753PheIle: 2.753 ± 0.05	1.774PheLys: 1.774 ± 0.044	3.829PheLeu: 3.829 ± 0.063	1.035PheMet: 1.035 ± 0.032	1.991PheAsn: 1.991 ± 0.043	1.662PhePro: 1.662 ± 0.04	1.346PheGln: 1.346 ± 0.035	1.953PheArg: 1.953 ± 0.048	3.12PheSer: 3.12 ± 0.05	2.31PheThr: 2.31 ± 0.048	2.76PheVal: 2.76 ± 0.054	0.563PheTrp: 0.563 ± 0.022	1.405PheTyr: 1.405 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.171GlyAla: 5.171 ± 0.097	0.822GlyCys: 0.822 ± 0.029	3.355GlyAsp: 3.355 ± 0.059	3.81GlyGlu: 3.81 ± 0.068	3.21GlyPhe: 3.21 ± 0.064	4.973GlyGly: 4.973 ± 0.105	1.738GlyHis: 1.738 ± 0.043	5.067GlyIle: 5.067 ± 0.072	3.937GlyLys: 3.937 ± 0.071	6.561GlyLeu: 6.561 ± 0.078	1.985GlyMet: 1.985 ± 0.045	2.984GlyAsn: 2.984 ± 0.063	1.699GlyPro: 1.699 ± 0.041	2.485GlyGln: 2.485 ± 0.054	3.533GlyArg: 3.533 ± 0.059	3.999GlySer: 3.999 ± 0.079	3.589GlyThr: 3.589 ± 0.063	4.619GlyVal: 4.619 ± 0.069	0.974GlyTrp: 0.974 ± 0.034	2.366GlyTyr: 2.366 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.195HisAla: 2.195 ± 0.049	0.311HisCys: 0.311 ± 0.016	1.438HisAsp: 1.438 ± 0.036	1.487HisGlu: 1.487 ± 0.042	1.127HisPhe: 1.127 ± 0.032	1.718HisGly: 1.718 ± 0.039	0.842HisHis: 0.842 ± 0.032	1.626HisIle: 1.626 ± 0.036	1.083HisLys: 1.083 ± 0.028	2.449HisLeu: 2.449 ± 0.051	0.582HisMet: 0.582 ± 0.023	1.013HisAsn: 1.013 ± 0.035	1.31HisPro: 1.31 ± 0.037	1.127HisGln: 1.127 ± 0.033	1.247HisArg: 1.247 ± 0.035	1.408HisSer: 1.408 ± 0.034	1.307HisThr: 1.307 ± 0.033	1.534HisVal: 1.534 ± 0.041	0.372HisTrp: 0.372 ± 0.021	0.905HisTyr: 0.905 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.538IleAla: 6.538 ± 0.092	0.77IleCys: 0.77 ± 0.028	4.331IleAsp: 4.331 ± 0.074	4.715IleGlu: 4.715 ± 0.078	2.678IlePhe: 2.678 ± 0.052	4.68IleGly: 4.68 ± 0.074	1.705IleHis: 1.705 ± 0.038	4.412IleIle: 4.412 ± 0.074	3.665IleLys: 3.665 ± 0.064	6.441IleLeu: 6.441 ± 0.092	1.528IleMet: 1.528 ± 0.042	3.403IleAsn: 3.403 ± 0.064	3.228IlePro: 3.228 ± 0.061	2.685IleGln: 2.685 ± 0.051	3.617IleArg: 3.617 ± 0.05	4.617IleSer: 4.617 ± 0.064	4.151IleThr: 4.151 ± 0.067	4.336IleVal: 4.336 ± 0.067	0.74IleTrp: 0.74 ± 0.031	1.896IleTyr: 1.896 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.036LysAla: 4.036 ± 0.069	0.365LysCys: 0.365 ± 0.021	2.348LysAsp: 2.348 ± 0.057	2.991LysGlu: 2.991 ± 0.055	1.607LysPhe: 1.607 ± 0.04	2.644LysGly: 2.644 ± 0.056	1.332LysHis: 1.332 ± 0.038	3.781LysIle: 3.781 ± 0.062	3.338LysLys: 3.338 ± 0.069	5.17LysLeu: 5.17 ± 0.078	1.377LysMet: 1.377 ± 0.037	2.695LysAsn: 2.695 ± 0.055	2.41LysPro: 2.41 ± 0.049	2.666LysGln: 2.666 ± 0.052	2.923LysArg: 2.923 ± 0.067	3.145LysSer: 3.145 ± 0.061	3.105LysThr: 3.105 ± 0.065	2.828LysVal: 2.828 ± 0.052	0.594LysTrp: 0.594 ± 0.022	1.358LysTyr: 1.358 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	9.121LeuAla: 9.121 ± 0.101	1.052LeuCys: 1.052 ± 0.034	5.588LeuAsp: 5.588 ± 0.076	5.849LeuGlu: 5.849 ± 0.086	4.176LeuPhe: 4.176 ± 0.069	6.512LeuGly: 6.512 ± 0.086	2.398LeuHis: 2.398 ± 0.05	6.874LeuIle: 6.874 ± 0.102	5.153LeuLys: 5.153 ± 0.072	10.64LeuLeu: 10.64 ± 0.158	2.437LeuMet: 2.437 ± 0.047	4.553LeuAsn: 4.553 ± 0.061	4.988LeuPro: 4.988 ± 0.072	4.285LeuGln: 4.285 ± 0.072	5.466LeuArg: 5.466 ± 0.079	7.22LeuSer: 7.22 ± 0.092	5.924LeuThr: 5.924 ± 0.067	6.159LeuVal: 6.159 ± 0.086	1.121LeuTrp: 1.121 ± 0.039	2.639LeuTyr: 2.639 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.217MetAla: 2.217 ± 0.045	0.191MetCys: 0.191 ± 0.014	1.248MetAsp: 1.248 ± 0.037	1.362MetGlu: 1.362 ± 0.033	0.848MetPhe: 0.848 ± 0.026	1.565MetGly: 1.565 ± 0.041	0.688MetHis: 0.688 ± 0.026	1.643MetIle: 1.643 ± 0.041	1.402MetLys: 1.402 ± 0.04	2.812MetLeu: 2.812 ± 0.058	0.652MetMet: 0.652 ± 0.03	1.238MetAsn: 1.238 ± 0.028	1.314MetPro: 1.314 ± 0.036	1.237MetGln: 1.237 ± 0.037	1.429MetArg: 1.429 ± 0.037	1.607MetSer: 1.607 ± 0.038	1.574MetThr: 1.574 ± 0.04	1.591MetVal: 1.591 ± 0.041	0.169MetTrp: 0.169 ± 0.013	0.476MetTyr: 0.476 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.702AsnAla: 3.702 ± 0.061	0.46AsnCys: 0.46 ± 0.022	2.329AsnAsp: 2.329 ± 0.052	2.477AsnGlu: 2.477 ± 0.046	1.723AsnPhe: 1.723 ± 0.048	2.945AsnGly: 2.945 ± 0.065	1.146AsnHis: 1.146 ± 0.033	3.093AsnIle: 3.093 ± 0.047	2.415AsnLys: 2.415 ± 0.05	4.302AsnLeu: 4.302 ± 0.066	1.054AsnMet: 1.054 ± 0.034	2.329AsnAsn: 2.329 ± 0.057	2.365AsnPro: 2.365 ± 0.052	2.069AsnGln: 2.069 ± 0.046	2.477AsnArg: 2.477 ± 0.051	2.58AsnSer: 2.58 ± 0.071	2.516AsnThr: 2.516 ± 0.045	2.534AsnVal: 2.534 ± 0.058	0.682AsnTrp: 0.682 ± 0.024	1.394AsnTyr: 1.394 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.498ProAla: 3.498 ± 0.06	0.346ProCys: 0.346 ± 0.016	3.07ProAsp: 3.07 ± 0.053	3.316ProGlu: 3.316 ± 0.058	1.776ProPhe: 1.776 ± 0.038	3.142ProGly: 3.142 ± 0.052	0.975ProHis: 0.975 ± 0.029	2.524ProIle: 2.524 ± 0.045	1.714ProLys: 1.714 ± 0.042	3.957ProLeu: 3.957 ± 0.063	0.96ProMet: 0.96 ± 0.034	1.571ProAsn: 1.571 ± 0.035	1.648ProPro: 1.648 ± 0.041	1.599ProGln: 1.599 ± 0.036	1.762ProArg: 1.762 ± 0.038	2.287ProSer: 2.287 ± 0.05	1.964ProThr: 1.964 ± 0.05	3.729ProVal: 3.729 ± 0.064	0.534ProTrp: 0.534 ± 0.026	1.279ProTyr: 1.279 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.886GlnAla: 3.886 ± 0.063	0.343GlnCys: 0.343 ± 0.019	1.909GlnAsp: 1.909 ± 0.043	2.248GlnGlu: 2.248 ± 0.049	1.756GlnPhe: 1.756 ± 0.038	2.47GlnGly: 2.47 ± 0.052	1.217GlnHis: 1.217 ± 0.036	3.078GlnIle: 3.078 ± 0.051	2.331GlnLys: 2.331 ± 0.049	4.856GlnLeu: 4.856 ± 0.074	1.04GlnMet: 1.04 ± 0.031	1.948GlnAsn: 1.948 ± 0.043	1.778GlnPro: 1.778 ± 0.044	2.507GlnGln: 2.507 ± 0.056	2.467GlnArg: 2.467 ± 0.052	2.754GlnSer: 2.754 ± 0.054	2.362GlnThr: 2.362 ± 0.051	2.654GlnVal: 2.654 ± 0.053	0.6GlnTrp: 0.6 ± 0.024	1.235GlnTyr: 1.235 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.943ArgAla: 3.943 ± 0.065	0.514ArgCys: 0.514 ± 0.023	2.727ArgAsp: 2.727 ± 0.046	3.404ArgGlu: 3.404 ± 0.06	2.48ArgPhe: 2.48 ± 0.05	2.88ArgGly: 2.88 ± 0.055	1.469ArgHis: 1.469 ± 0.036	4.247ArgIle: 4.247 ± 0.061	2.913ArgLys: 2.913 ± 0.059	5.887ArgLeu: 5.887 ± 0.086	1.479ArgMet: 1.479 ± 0.035	2.428ArgAsn: 2.428 ± 0.046	1.894ArgPro: 1.894 ± 0.041	2.517ArgGln: 2.517 ± 0.051	2.983ArgArg: 2.983 ± 0.069	2.971ArgSer: 2.971 ± 0.055	2.612ArgThr: 2.612 ± 0.058	3.278ArgVal: 3.278 ± 0.058	0.705ArgTrp: 0.705 ± 0.023	2.078ArgTyr: 2.078 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.22SerAla: 5.22 ± 0.075	0.647SerCys: 0.647 ± 0.025	3.566SerAsp: 3.566 ± 0.065	3.654SerGlu: 3.654 ± 0.068	2.641SerPhe: 2.641 ± 0.045	5.211SerGly: 5.211 ± 0.08	1.396SerHis: 1.396 ± 0.038	4.355SerIle: 4.355 ± 0.068	2.826SerLys: 2.826 ± 0.054	6.102SerLeu: 6.102 ± 0.086	1.616SerMet: 1.616 ± 0.036	2.751SerAsn: 2.751 ± 0.064	2.502SerPro: 2.502 ± 0.048	2.475SerGln: 2.475 ± 0.041	3.232SerArg: 3.232 ± 0.055	4.03SerSer: 4.03 ± 0.08	3.306SerThr: 3.306 ± 0.057	4.294SerVal: 4.294 ± 0.058	0.762SerTrp: 0.762 ± 0.025	1.672SerTyr: 1.672 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	4.849ThrAla: 4.849 ± 0.076	0.54ThrCys: 0.54 ± 0.024	3.2ThrAsp: 3.2 ± 0.057	3.219ThrGlu: 3.219 ± 0.058	2.112ThrPhe: 2.112 ± 0.041	4.565ThrGly: 4.565 ± 0.076	1.428ThrHis: 1.428 ± 0.033	3.735ThrIle: 3.735 ± 0.057	2.217ThrLys: 2.217 ± 0.046	5.996ThrLeu: 5.996 ± 0.084	1.167ThrMet: 1.167 ± 0.035	2.05ThrAsn: 2.05 ± 0.049	2.673ThrPro: 2.673 ± 0.053	2.332ThrGln: 2.332 ± 0.046	2.794ThrArg: 2.794 ± 0.054	3.187ThrSer: 3.187 ± 0.067	3.018ThrThr: 3.018 ± 0.064	3.982ThrVal: 3.982 ± 0.066	0.614ThrTrp: 0.614 ± 0.028	1.512ThrTyr: 1.512 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	5.63ValAla: 5.63 ± 0.084	0.681ValCys: 0.681 ± 0.026	3.653ValAsp: 3.653 ± 0.05	3.707ValGlu: 3.707 ± 0.058	2.761ValPhe: 2.761 ± 0.058	3.979ValGly: 3.979 ± 0.06	1.483ValHis: 1.483 ± 0.043	4.807ValIle: 4.807 ± 0.077	3.08ValLys: 3.08 ± 0.063	6.541ValLeu: 6.541 ± 0.083	1.816ValMet: 1.816 ± 0.045	2.854ValAsn: 2.854 ± 0.05	2.629ValPro: 2.629 ± 0.044	2.401ValGln: 2.401 ± 0.05	3.328ValArg: 3.328 ± 0.057	4.428ValSer: 4.428 ± 0.058	3.897ValThr: 3.897 ± 0.064	4.365ValVal: 4.365 ± 0.08	0.771ValTrp: 0.771 ± 0.025	1.817ValTyr: 1.817 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.807TrpAla: 0.807 ± 0.028	0.152TrpCys: 0.152 ± 0.012	0.553TrpAsp: 0.553 ± 0.023	0.63TrpGlu: 0.63 ± 0.026	0.552TrpPhe: 0.552 ± 0.022	0.673TrpGly: 0.673 ± 0.028	0.442TrpHis: 0.442 ± 0.022	0.912TrpIle: 0.912 ± 0.026	0.603TrpLys: 0.603 ± 0.023	1.744TrpLeu: 1.744 ± 0.043	0.357TrpMet: 0.357 ± 0.02	0.549TrpAsn: 0.549 ± 0.021	0.485TrpPro: 0.485 ± 0.025	0.793TrpGln: 0.793 ± 0.03	0.874TrpArg: 0.874 ± 0.028	0.696TrpSer: 0.696 ± 0.026	0.568TrpThr: 0.568 ± 0.024	0.785TrpVal: 0.785 ± 0.026	0.202TrpTrp: 0.202 ± 0.014	0.351TrpTyr: 0.351 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.525TyrAla: 2.525 ± 0.051	0.354TyrCys: 0.354 ± 0.016	1.654TyrAsp: 1.654 ± 0.047	1.564TyrGlu: 1.564 ± 0.039	1.404TyrPhe: 1.404 ± 0.04	2.121TyrGly: 2.121 ± 0.043	0.837TyrHis: 0.837 ± 0.029	1.68TyrIle: 1.68 ± 0.043	1.23TyrLys: 1.23 ± 0.03	3.165TyrLeu: 3.165 ± 0.055	0.598TyrMet: 0.598 ± 0.026	1.121TyrAsn: 1.121 ± 0.038	1.413TyrPro: 1.413 ± 0.039	1.57TyrGln: 1.57 ± 0.038	2.006TyrArg: 2.006 ± 0.044	1.753TyrSer: 1.753 ± 0.042	1.6TyrThr: 1.6 ± 0.035	1.754TyrVal: 1.754 ± 0.044	0.469TyrTrp: 0.469 ± 0.018	1.019TyrTyr: 1.019 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3744 proteins (1122864 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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