Burkholderia phage Bcep176

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3HQV7|Q3HQV7_9CAUD Gp50 OS=Burkholderia phage Bcep176 OX=348137 GN=50 PE=4 SV=1
MM1 pKa = 7.91SDD3 pKa = 3.95TDD5 pKa = 4.68DD6 pKa = 4.74DD7 pKa = 3.65WTEE10 pKa = 3.93PEE12 pKa = 4.41KK13 pKa = 11.12EE14 pKa = 3.95IDD16 pKa = 4.07DD17 pKa = 4.61GYY19 pKa = 11.52ARR21 pKa = 11.84CPHH24 pKa = 6.64GMPKK28 pKa = 9.23WDD30 pKa = 4.26CDD32 pKa = 3.12WCRR35 pKa = 11.84EE36 pKa = 4.15SEE38 pKa = 4.35DD39 pKa = 3.37IEE41 pKa = 4.58RR42 pKa = 11.84VGGTSEE48 pKa = 3.82

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3HQX5|Q3HQX5_9CAUD Gp32 OS=Burkholderia phage Bcep176 OX=348137 GN=32 PE=4 SV=1
MM1 pKa = 7.68LPRR4 pKa = 11.84GSRR7 pKa = 11.84RR8 pKa = 11.84TSCTRR13 pKa = 11.84CSGKK17 pKa = 10.63GGGPVGYY24 pKa = 7.65VHH26 pKa = 7.65RR27 pKa = 11.84MQSPNKK33 pKa = 9.38QSSTQGPPLEE43 pKa = 4.47ALNVLKK49 pKa = 10.8NLTLDD54 pKa = 3.18YY55 pKa = 9.17WYY57 pKa = 11.34KK58 pKa = 10.21MLIPLALGLFVLAITVDD75 pKa = 3.51LRR77 pKa = 11.84VISNGSLVLLAIGLFMIGLGEE98 pKa = 4.56WINHH102 pKa = 5.31PRR104 pKa = 11.84QEE106 pKa = 5.2AINLHH111 pKa = 6.5LNMKK115 pKa = 8.59ITLHH119 pKa = 5.25RR120 pKa = 11.84WKK122 pKa = 10.16PKK124 pKa = 9.55AWGIALDD131 pKa = 3.83LAGAVLGGIGFFGIVRR147 pKa = 11.84AAIRR151 pKa = 11.84AAAA154 pKa = 3.9

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

14209

24

1380

175.4

19.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.681 ± 0.791

0.985 ± 0.158

5.94 ± 0.181

5.743 ± 0.34

3.054 ± 0.165

8.171 ± 0.271

1.626 ± 0.169

4.751 ± 0.243

4.173 ± 0.243

7.615 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.136

3.125 ± 0.181

4.736 ± 0.373

3.814 ± 0.204

7.699 ± 0.378

6.045 ± 0.275

5.321 ± 0.241

6.756 ± 0.36

1.788 ± 0.179

2.428 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski