Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Syntrophotaleaceae; Syntrophotalea; Syntrophotalea carbinolica

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3292 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3A7F4|Q3A7F4_PELCD SGNH-hydrolase lipoprotein lysophospholipase L1-like subgroup OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=Pcar_0430 PE=4 SV=2
MM1 pKa = 7.37KK2 pKa = 9.77KK3 pKa = 9.7WSVLLVSVFMLIAGFASSATAAIEE27 pKa = 4.24VEE29 pKa = 3.65GDD31 pKa = 3.14AYY33 pKa = 10.83AGIWDD38 pKa = 3.72KK39 pKa = 11.76YY40 pKa = 6.29MWRR43 pKa = 11.84GFNLSDD49 pKa = 4.46SRR51 pKa = 11.84PTIQAGVDD59 pKa = 3.5LTLGGGFTLSTWHH72 pKa = 5.97NWQLTGGDD80 pKa = 3.36NWPAGEE86 pKa = 4.39LNEE89 pKa = 4.17TDD91 pKa = 4.21VILTYY96 pKa = 10.46AYY98 pKa = 10.26DD99 pKa = 3.86VNDD102 pKa = 5.53LISVSIGDD110 pKa = 3.37IWYY113 pKa = 8.74MIEE116 pKa = 5.12GEE118 pKa = 4.24DD119 pKa = 3.93TNEE122 pKa = 3.75LFATITLNTLLSPNLKK138 pKa = 9.72ISYY141 pKa = 9.61DD142 pKa = 3.24WDD144 pKa = 3.58RR145 pKa = 11.84AEE147 pKa = 4.09EE148 pKa = 4.56DD149 pKa = 3.71GLFFSFDD156 pKa = 3.15ISHH159 pKa = 6.71SFDD162 pKa = 3.0LTQWVPDD169 pKa = 3.48TTLNLGALVSYY180 pKa = 9.58NQHH183 pKa = 6.5ADD185 pKa = 3.32GTVADD190 pKa = 3.75YY191 pKa = 11.31SGWHH195 pKa = 6.07NYY197 pKa = 7.78EE198 pKa = 4.07LSASLDD204 pKa = 3.66YY205 pKa = 11.53ALTDD209 pKa = 3.42QLTLSPIVIFSSPISSAAKK228 pKa = 9.77EE229 pKa = 4.22LIDD232 pKa = 4.03TEE234 pKa = 4.31SAAALNLTFTFF245 pKa = 4.37

Molecular weight: 27.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3A057|Q3A057_PELCD Magnesium-dependent deoxyribonuclease TatD family OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=Pcar_3015 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.94RR4 pKa = 11.84TYY6 pKa = 9.06QPSRR10 pKa = 11.84IRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.9RR15 pKa = 11.84THH17 pKa = 6.1GFRR20 pKa = 11.84SRR22 pKa = 11.84MQTKK26 pKa = 9.97NGQAVIRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.65RR42 pKa = 11.84LVVTIPVKK50 pKa = 10.76

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3292

0

3292

1056071

30

1925

320.8

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.478 ± 0.049

1.422 ± 0.019

5.55 ± 0.035

6.314 ± 0.041

3.949 ± 0.026

7.865 ± 0.043

2.251 ± 0.019

5.55 ± 0.037

4.328 ± 0.041

11.093 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.019

3.052 ± 0.026

4.614 ± 0.027

3.92 ± 0.028

6.728 ± 0.04

5.48 ± 0.028

4.948 ± 0.024

7.206 ± 0.033

1.113 ± 0.017

2.625 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski