Prochlorococcus phage Syn1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vellamovirus; Prochlorococcus virus Syn1

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SPF0|E3SPF0_9CAUD Uncharacterized protein OS=Prochlorococcus phage Syn1 OX=444861 GN=Syn1_065 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.98LHH5 pKa = 8.55DD6 pKa = 4.85SDD8 pKa = 5.79CCDD11 pKa = 3.4DD12 pKa = 4.41HH13 pKa = 8.8LQFEE17 pKa = 4.63QLTLDD22 pKa = 3.48VTII25 pKa = 5.0

Molecular weight: 2.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SPG1|E3SPG1_9CAUD Uncharacterized protein OS=Prochlorococcus phage Syn1 OX=444861 GN=Syn1_076 PE=4 SV=1
MM1 pKa = 7.35TPCKK5 pKa = 10.2LSQPPRR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 8.91SEE15 pKa = 4.1SYY17 pKa = 10.54GRR19 pKa = 11.84IQRR22 pKa = 11.84SHH24 pKa = 6.6RR25 pKa = 11.84GKK27 pKa = 10.26EE28 pKa = 3.67RR29 pKa = 11.84RR30 pKa = 11.84LSSFCEE36 pKa = 3.96FSKK39 pKa = 10.76SVKK42 pKa = 9.62YY43 pKa = 10.25QNSKK47 pKa = 10.58NLTFF51 pKa = 4.77

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

62099

25

4017

265.4

29.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.754 ± 0.217

0.873 ± 0.076

6.652 ± 0.119

6.003 ± 0.247

4.461 ± 0.101

7.95 ± 0.35

1.462 ± 0.117

6.449 ± 0.151

5.504 ± 0.383

7.219 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.064 ± 0.19

6.06 ± 0.194

4.024 ± 0.124

3.691 ± 0.101

4.018 ± 0.112

7.359 ± 0.213

7.615 ± 0.324

6.728 ± 0.169

1.079 ± 0.08

4.034 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski