Marininema mesophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Marininema

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3C054|A0A1H3C054_9BACL RNA polymerase sigma-70 factor ECF subfamily OS=Marininema mesophilum OX=1048340 GN=SAMN05444487_1196 PE=3 SV=1
MM1 pKa = 8.04KK2 pKa = 9.68MDD4 pKa = 4.33LEE6 pKa = 4.51DD7 pKa = 3.44WLDD10 pKa = 4.01IIVSGAAFIGLVVVWTVLLILYY32 pKa = 8.52GCC34 pKa = 4.67

Molecular weight: 3.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2VET9|A0A1H2VET9_9BACL Aspartate--tRNA ligase OS=Marininema mesophilum OX=1048340 GN=aspS PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84RR3 pKa = 11.84LISSLLIGSIITLLYY18 pKa = 10.21RR19 pKa = 11.84RR20 pKa = 11.84FRR22 pKa = 11.84RR23 pKa = 11.84PTSIAGWMNRR33 pKa = 11.84RR34 pKa = 11.84MGGMSWMDD42 pKa = 3.0IPRR45 pKa = 11.84WLRR48 pKa = 11.84KK49 pKa = 9.8LPIKK53 pKa = 10.0TNGARR58 pKa = 11.84IISMVIGRR66 pKa = 11.84KK67 pKa = 6.85WFRR70 pKa = 11.84KK71 pKa = 6.52MARR74 pKa = 3.41

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3137

0

3137

942030

26

4094

300.3

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.425 ± 0.053

0.824 ± 0.011

5.063 ± 0.036

7.064 ± 0.06

3.967 ± 0.033

7.644 ± 0.049

2.279 ± 0.028

6.627 ± 0.039

5.735 ± 0.055

10.115 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.024

3.451 ± 0.032

4.295 ± 0.034

3.938 ± 0.032

5.601 ± 0.047

6.034 ± 0.035

5.506 ± 0.035

7.331 ± 0.043

1.342 ± 0.02

3.061 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski