Robiginitalea myxolifaciens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Robiginitalea

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1I6HER6|A0A1I6HER6_9FLAO Polyphosphate glucokinase OS=Robiginitalea myxolifaciens OX=400055 GN=SAMN04490243_2660 PE=4 SV=1
MM1 pKa = 6.99TRR3 pKa = 11.84NLVFLLAFLWATYY16 pKa = 10.57SLQAQNSPDD25 pKa = 3.77CRR27 pKa = 11.84SAIPVCADD35 pKa = 3.2APILNFADD43 pKa = 3.73GGGDD47 pKa = 3.65IDD49 pKa = 6.32DD50 pKa = 5.3FDD52 pKa = 6.72PDD54 pKa = 3.83VIRR57 pKa = 11.84QSGCLEE63 pKa = 4.22KK64 pKa = 11.13GSVASANIEE73 pKa = 4.33NNTSWYY79 pKa = 9.78VFRR82 pKa = 11.84AGTGGQVGFDD92 pKa = 3.42IEE94 pKa = 4.45ALSDD98 pKa = 3.27TAEE101 pKa = 4.0WDD103 pKa = 3.62FALYY107 pKa = 10.5GPDD110 pKa = 3.83VDD112 pKa = 5.97CGDD115 pKa = 3.91ISNGTAQPIRR125 pKa = 11.84CNYY128 pKa = 8.15EE129 pKa = 3.76VNDD132 pKa = 3.71TRR134 pKa = 11.84FTGVGVNPEE143 pKa = 4.02NGQAGQPFVKK153 pKa = 10.48GSQNTYY159 pKa = 10.82DD160 pKa = 3.21EE161 pKa = 5.16WIDD164 pKa = 3.66VQPGEE169 pKa = 4.7IYY171 pKa = 10.83YY172 pKa = 10.82LLINNYY178 pKa = 7.43NTNFDD183 pKa = 4.4GDD185 pKa = 4.2PEE187 pKa = 4.72PYY189 pKa = 10.57SLTFTGNSVDD199 pKa = 4.05ADD201 pKa = 3.31QDD203 pKa = 3.66NALDD207 pKa = 3.68CTLRR211 pKa = 11.84DD212 pKa = 3.55EE213 pKa = 5.22FLGLDD218 pKa = 3.54IVACEE223 pKa = 4.1GDD225 pKa = 3.61PDD227 pKa = 3.97IVLSALNSPAGPDD240 pKa = 3.07IANVTWSVDD249 pKa = 3.54YY250 pKa = 11.19EE251 pKa = 4.36DD252 pKa = 5.73DD253 pKa = 4.42GVIDD257 pKa = 4.09AQLADD262 pKa = 4.17GPGEE266 pKa = 4.18TEE268 pKa = 4.02FTVVSPISGRR278 pKa = 11.84YY279 pKa = 7.43YY280 pKa = 11.07VEE282 pKa = 3.8ILTTLATTITDD293 pKa = 4.65DD294 pKa = 5.89ILITWYY300 pKa = 10.27GVPVLDD306 pKa = 5.39RR307 pKa = 11.84VDD309 pKa = 3.81ILDD312 pKa = 5.41DD313 pKa = 5.69LSDD316 pKa = 3.53QNNIQVFVQGDD327 pKa = 3.6GDD329 pKa = 4.16YY330 pKa = 11.45EE331 pKa = 3.93FAINNGPFQDD341 pKa = 4.19DD342 pKa = 4.25SIFRR346 pKa = 11.84DD347 pKa = 3.94VPPGINTLIINDD359 pKa = 3.81KK360 pKa = 9.92NGCGTTEE367 pKa = 4.52PIEE370 pKa = 4.07FLVVGYY376 pKa = 9.34PKK378 pKa = 10.75FFTPNNDD385 pKa = 3.17TFNDD389 pKa = 3.21TWQVKK394 pKa = 10.18GIEE397 pKa = 4.13TLIDD401 pKa = 3.4PVVFIFDD408 pKa = 3.99RR409 pKa = 11.84YY410 pKa = 10.64GKK412 pKa = 9.9LLKK415 pKa = 10.74QIDD418 pKa = 4.21EE419 pKa = 4.68TSLGWDD425 pKa = 3.34GSFNGRR431 pKa = 11.84PMPASDD437 pKa = 2.97YY438 pKa = 10.13WFRR441 pKa = 11.84LEE443 pKa = 3.92YY444 pKa = 10.89SRR446 pKa = 11.84DD447 pKa = 3.15EE448 pKa = 4.33SGIVVANTIRR458 pKa = 11.84AHH460 pKa = 5.41FTLKK464 pKa = 10.46RR465 pKa = 3.52

Molecular weight:
51.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1I6FMU4|A0A1I6FMU4_9FLAO DUF4328 domain-containing protein OS=Robiginitalea myxolifaciens OX=400055 GN=SAMN04490243_0087 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.27RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.52LSVSSEE47 pKa = 3.87PRR49 pKa = 11.84HH50 pKa = 5.89KK51 pKa = 10.65RR52 pKa = 3.21

Molecular weight:
6.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2844

0

2844

998853

31

4472

351.2

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.924 ± 0.044

0.728 ± 0.015

5.703 ± 0.051

6.956 ± 0.052

4.821 ± 0.033

7.533 ± 0.052

1.799 ± 0.023

6.443 ± 0.033

5.152 ± 0.058

10.182 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.024

4.554 ± 0.041

4.218 ± 0.034

3.801 ± 0.027

5.063 ± 0.044

6.231 ± 0.037

5.393 ± 0.06

6.278 ± 0.039

1.22 ± 0.018

3.724 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski