Zucchini green mottle mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q911R3|Q911R3_9VIRU Isoform of Q911R4 131 kDa replicase OS=Zucchini green mottle mosaic virus OX=111418 GN=replicase PE=4 SV=1
MM1 pKa = 7.92PYY3 pKa = 8.35STSGIRR9 pKa = 11.84SLPAFSKK16 pKa = 11.02SFFPYY21 pKa = 10.64LEE23 pKa = 4.89LYY25 pKa = 10.37NLLITNQGAALQTQNGKK42 pKa = 10.39DD43 pKa = 3.3ILRR46 pKa = 11.84EE47 pKa = 4.02SLVGLLSSVASPTSQFPSGVFYY69 pKa = 10.62VWSRR73 pKa = 11.84EE74 pKa = 3.84SRR76 pKa = 11.84IAALIDD82 pKa = 3.7SLFGALDD89 pKa = 3.35SRR91 pKa = 11.84NRR93 pKa = 11.84AIEE96 pKa = 3.87VEE98 pKa = 4.25NPSNPSTGEE107 pKa = 3.45ALNAVKK113 pKa = 10.64RR114 pKa = 11.84NDD116 pKa = 3.89DD117 pKa = 3.5ASTAAHH123 pKa = 6.6NDD125 pKa = 2.72IPQILSALNEE135 pKa = 4.14GAGVFDD141 pKa = 3.78RR142 pKa = 11.84ASFEE146 pKa = 4.56SAFGLVWTAGSSTSSS161 pKa = 3.03

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q911R4|Q911R4_9VIRU Methyltransferase/RNA helicase OS=Zucchini green mottle mosaic virus OX=111418 GN=replicase PE=4 SV=1
MM1 pKa = 7.34SVSKK5 pKa = 11.03LGVKK9 pKa = 9.91NVLKK13 pKa = 9.88PQEE16 pKa = 4.17FVKK19 pKa = 10.84LNWVDD24 pKa = 3.84KK25 pKa = 10.35VLPDD29 pKa = 3.29MFTVFRR35 pKa = 11.84YY36 pKa = 10.24LSVTDD41 pKa = 3.3YY42 pKa = 11.59SVIKK46 pKa = 10.86SRR48 pKa = 11.84DD49 pKa = 3.78SEE51 pKa = 4.39CLIPVDD57 pKa = 4.3LLRR60 pKa = 11.84GVDD63 pKa = 3.87LSKK66 pKa = 10.97SKK68 pKa = 10.94YY69 pKa = 6.73VTLVGVVISGVWTIPEE85 pKa = 4.03NCAGGATVALVDD97 pKa = 3.8TRR99 pKa = 11.84MSMVDD104 pKa = 3.27EE105 pKa = 4.13GTICKK110 pKa = 10.41FSVAASARR118 pKa = 11.84DD119 pKa = 3.72FMVKK123 pKa = 10.45LIPNYY128 pKa = 10.46YY129 pKa = 8.55VTATDD134 pKa = 3.9ASSKK138 pKa = 9.08PWSIFVRR145 pKa = 11.84VSGVRR150 pKa = 11.84IKK152 pKa = 10.89EE153 pKa = 4.11GFSPLTLEE161 pKa = 4.2IASLVATTNSILKK174 pKa = 9.83KK175 pKa = 9.05GLRR178 pKa = 11.84VSVLEE183 pKa = 4.18SVVGSDD189 pKa = 3.59ASVNLEE195 pKa = 3.98SASEE199 pKa = 4.07KK200 pKa = 10.0VQPFFDD206 pKa = 4.12SVPITAAVISRR217 pKa = 11.84DD218 pKa = 3.03RR219 pKa = 11.84SYY221 pKa = 11.66VSKK224 pKa = 10.93SGFNRR229 pKa = 11.84AVRR232 pKa = 11.84SKK234 pKa = 10.31PPSKK238 pKa = 10.44GGKK241 pKa = 9.54KK242 pKa = 10.29FGDD245 pKa = 3.78SAEE248 pKa = 4.29SLSEE252 pKa = 4.16DD253 pKa = 3.93SASEE257 pKa = 4.22LPGLL261 pKa = 4.37

Molecular weight: 28.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

3258

161

1670

814.5

91.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.366 ± 0.435

1.995 ± 0.338

6.108 ± 0.254

5.648 ± 0.228

5.586 ± 0.337

4.665 ± 0.457

2.271 ± 0.501

5.218 ± 0.119

6.077 ± 0.458

8.257 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.177

3.591 ± 0.284

3.959 ± 0.204

3.376 ± 0.439

4.696 ± 0.069

8.84 ± 1.465

6.476 ± 0.407

9.269 ± 0.696

1.197 ± 0.007

3.376 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski