Lactococcus phage C41431

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BMK3|A0A1P8BMK3_9CAUD Holin OS=Lactococcus phage C41431 OX=1868861 GN=C41431_51 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.11KK3 pKa = 8.64YY4 pKa = 9.83WVVEE8 pKa = 3.9DD9 pKa = 3.74HH10 pKa = 6.88LGGGFYY16 pKa = 10.8LMPEE20 pKa = 4.1DD21 pKa = 4.04TPEE24 pKa = 3.89EE25 pKa = 3.99EE26 pKa = 4.03LRR28 pKa = 11.84EE29 pKa = 4.04VEE31 pKa = 5.18VYY33 pKa = 10.96CDD35 pKa = 3.23TCGDD39 pKa = 3.54NDD41 pKa = 4.7SIIGQFSNWNQLKK54 pKa = 10.45KK55 pKa = 11.08EE56 pKa = 4.06MTDD59 pKa = 3.5DD60 pKa = 4.56EE61 pKa = 5.2GWCPYY66 pKa = 10.28SDD68 pKa = 5.14EE69 pKa = 4.2YY70 pKa = 10.46LQSVFEE76 pKa = 4.68EE77 pKa = 4.75DD78 pKa = 3.42NQQ80 pKa = 3.87

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BMQ0|A0A1P8BMQ0_9CAUD Receptor binding protein OS=Lactococcus phage C41431 OX=1868861 GN=C41431_48 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 10.31NLFKK7 pKa = 11.11LNRR10 pKa = 11.84SGVASMMKK18 pKa = 9.93SPEE21 pKa = 3.64MQAILKK27 pKa = 9.14EE28 pKa = 3.99KK29 pKa = 10.66ASAVKK34 pKa = 9.6QRR36 pKa = 11.84CGPGYY41 pKa = 9.68GQDD44 pKa = 2.96MHH46 pKa = 7.79VGKK49 pKa = 10.17NRR51 pKa = 11.84ANAMVFAEE59 pKa = 5.25TYY61 pKa = 7.86QAKK64 pKa = 9.74RR65 pKa = 11.84DD66 pKa = 3.63NMKK69 pKa = 10.98NNTILKK75 pKa = 9.9AVRR78 pKa = 3.37

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9230

59

857

181.0

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 0.411

0.769 ± 0.203

5.948 ± 0.314

6.934 ± 0.549

4.475 ± 0.203

6.674 ± 0.519

1.224 ± 0.125

6.685 ± 0.356

8.624 ± 0.509

7.887 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.319 ± 0.208

6.457 ± 0.357

3.218 ± 0.225

4.334 ± 0.433

3.781 ± 0.296

7.281 ± 0.355

6.241 ± 0.549

5.883 ± 0.254

1.257 ± 0.114

3.803 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski