Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Neisseria; Neisseria gonorrhoeae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q5F9H8|Q5F9H8_NEIG1 Periplasmic protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=NGO_0416 PE=4 SV=1
MM1 pKa = 7.49QILSFQADD9 pKa = 3.06IAEE12 pKa = 4.24RR13 pKa = 11.84MLEE16 pKa = 4.08GTEE19 pKa = 3.98GEE21 pKa = 4.62SVNEE25 pKa = 3.6NAQFVRR31 pKa = 11.84TDD33 pKa = 2.98NGYY36 pKa = 9.3WIAWHH41 pKa = 6.48EE42 pKa = 4.27GVAALLAPDD51 pKa = 4.56TPPGIPCFWVEE62 pKa = 3.99GAEE65 pKa = 4.43SLEE68 pKa = 4.18EE69 pKa = 3.82LCAMVEE75 pKa = 3.71RR76 pKa = 11.84GEE78 pKa = 3.97FDD80 pKa = 3.15EE81 pKa = 5.53VEE83 pKa = 4.38EE84 pKa = 5.19FDD86 pKa = 5.22GDD88 pKa = 3.62DD89 pKa = 4.49DD90 pKa = 4.35AWLEE94 pKa = 3.81AAKK97 pKa = 10.7DD98 pKa = 3.94CGHH101 pKa = 7.0HH102 pKa = 7.2GDD104 pKa = 3.97ACACGHH110 pKa = 6.54

Molecular weight:
12.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q5F519|Q5F519_NEIG1 Sulfate transporter OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=NGO_2120 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2106

0

2106

571507

29

1977

271.4

29.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.281 ± 0.076

1.103 ± 0.022

5.267 ± 0.043

6.164 ± 0.054

4.162 ± 0.045

7.896 ± 0.058

2.162 ± 0.027

5.734 ± 0.043

5.674 ± 0.052

9.735 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.025

4.007 ± 0.044

4.33 ± 0.041

3.838 ± 0.043

5.721 ± 0.05

5.424 ± 0.037

5.069 ± 0.033

6.78 ± 0.049

1.181 ± 0.022

2.966 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski