Halolamina pelagica

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halolamina

Average proteome isoelectric point is 5.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3464 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N8HZJ8|A0A0N8HZJ8_9EURY tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS OS=Halolamina pelagica OX=699431 GN=tiaS_1 PE=4 SV=1
MM1 pKa = 7.13TGPWAQWDD9 pKa = 4.0HH10 pKa = 5.13VLKK13 pKa = 10.77VDD15 pKa = 4.28PDD17 pKa = 3.82KK18 pKa = 11.45EE19 pKa = 4.13LLDD22 pKa = 5.48DD23 pKa = 3.81EE24 pKa = 5.11TFDD27 pKa = 5.25DD28 pKa = 4.5VAATGTDD35 pKa = 3.09ALEE38 pKa = 4.31IGGTMDD44 pKa = 3.22VTSEE48 pKa = 3.54KK49 pKa = 9.45MARR52 pKa = 11.84VVDD55 pKa = 4.03ACAAHH60 pKa = 6.99DD61 pKa = 3.74VPIYY65 pKa = 10.11QEE67 pKa = 3.88PSNPGVVIDD76 pKa = 5.06HH77 pKa = 6.84EE78 pKa = 4.56ALDD81 pKa = 4.95GYY83 pKa = 10.49LVPTVLNAGDD93 pKa = 4.26PFWITGAHH101 pKa = 6.22KK102 pKa = 9.33EE103 pKa = 3.86WARR106 pKa = 11.84IDD108 pKa = 5.82DD109 pKa = 4.47DD110 pKa = 5.66LDD112 pKa = 3.3WANTHH117 pKa = 5.25TEE119 pKa = 4.15AYY121 pKa = 10.2VVLNPDD127 pKa = 3.37AAVAEE132 pKa = 4.35YY133 pKa = 10.65TEE135 pKa = 4.81ADD137 pKa = 3.69CEE139 pKa = 4.27LTAEE143 pKa = 4.36EE144 pKa = 4.23VAAYY148 pKa = 10.24AGVAEE153 pKa = 4.72RR154 pKa = 11.84MFGQDD159 pKa = 2.44IVYY162 pKa = 10.51LEE164 pKa = 4.01YY165 pKa = 10.88SGTYY169 pKa = 10.14GDD171 pKa = 4.77PEE173 pKa = 4.4TVAAAADD180 pKa = 3.98EE181 pKa = 5.06LDD183 pKa = 4.47DD184 pKa = 3.83ATLFYY189 pKa = 11.24GGGIHH194 pKa = 7.56DD195 pKa = 4.3YY196 pKa = 10.92EE197 pKa = 4.79SARR200 pKa = 11.84EE201 pKa = 4.03MGEE204 pKa = 3.85HH205 pKa = 7.19ADD207 pKa = 3.82TVVVGDD213 pKa = 4.37LLHH216 pKa = 7.44DD217 pKa = 4.24EE218 pKa = 4.95GVDD221 pKa = 3.71AVAEE225 pKa = 4.36TVDD228 pKa = 3.65GAKK231 pKa = 10.47DD232 pKa = 3.13AA233 pKa = 4.74

Molecular weight: 25.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P7GV22|A0A0P7GV22_9EURY Uncharacterized protein OS=Halolamina pelagica OX=699431 GN=SY89_00086 PE=4 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 9.04SVRR7 pKa = 11.84VRR9 pKa = 11.84KK10 pKa = 9.71VRR12 pKa = 11.84YY13 pKa = 8.58KK14 pKa = 10.04GHH16 pKa = 4.63TVRR19 pKa = 11.84VKK21 pKa = 9.34VYY23 pKa = 9.36RR24 pKa = 11.84RR25 pKa = 11.84NGRR28 pKa = 11.84IVRR31 pKa = 11.84LKK33 pKa = 10.43VIKK36 pKa = 10.81SPVKK40 pKa = 9.86VKK42 pKa = 10.61RR43 pKa = 11.84KK44 pKa = 9.17VLIRR48 pKa = 11.84RR49 pKa = 11.84IRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 3.26

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3464

0

3464

838665

29

1422

242.1

26.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.781 ± 0.071

0.748 ± 0.016

8.054 ± 0.051

8.359 ± 0.065

3.248 ± 0.03

8.73 ± 0.045

1.938 ± 0.022

3.709 ± 0.033

1.668 ± 0.023

8.879 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.79 ± 0.017

2.174 ± 0.025

4.954 ± 0.031

2.436 ± 0.025

6.861 ± 0.05

5.62 ± 0.037

6.397 ± 0.039

8.917 ± 0.042

1.19 ± 0.018

2.547 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski