Microbacterium sp. TNHR37B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164AXD9|A0A164AXD9_9MICO Bifunctional protein PutA OS=Microbacterium sp. TNHR37B OX=1775956 GN=putA PE=3 SV=1
MM1 pKa = 7.59NKK3 pKa = 9.86RR4 pKa = 11.84LLAAAAAVAATALALSACSAGTAPKK29 pKa = 10.58GDD31 pKa = 3.88DD32 pKa = 4.45AIDD35 pKa = 4.41EE36 pKa = 4.27IRR38 pKa = 11.84VAATDD43 pKa = 3.75PQLIIPGRR51 pKa = 11.84QSVAYY56 pKa = 8.94DD57 pKa = 3.09VNMAVWAPLAFLNSDD72 pKa = 3.36GSLDD76 pKa = 3.77YY77 pKa = 11.24VQAEE81 pKa = 4.53SIEE84 pKa = 4.23SDD86 pKa = 4.57DD87 pKa = 5.26AITWTITLRR96 pKa = 11.84EE97 pKa = 4.02GWTFQDD103 pKa = 3.8GTPVTAQSYY112 pKa = 8.15VDD114 pKa = 3.3SWNAVAYY121 pKa = 9.93GPNAFEE127 pKa = 5.69NSGQLANIVGYY138 pKa = 11.18ADD140 pKa = 4.49LNPAEE145 pKa = 5.03GEE147 pKa = 4.02PSTTEE152 pKa = 3.63MSGLAVVDD160 pKa = 3.68EE161 pKa = 4.42RR162 pKa = 11.84TFTVEE167 pKa = 3.53LTGPDD172 pKa = 3.44SQFPLQVTQAQTAMFPMPASALTDD196 pKa = 3.82FDD198 pKa = 5.13AYY200 pKa = 9.28NTHH203 pKa = 7.62PIGNGPFAFAEE214 pKa = 4.42DD215 pKa = 4.09YY216 pKa = 11.29VEE218 pKa = 4.51NEE220 pKa = 4.4PIVLEE225 pKa = 5.35AYY227 pKa = 10.25DD228 pKa = 4.97DD229 pKa = 4.09YY230 pKa = 11.6QGEE233 pKa = 4.48KK234 pKa = 8.8PTIDD238 pKa = 3.95RR239 pKa = 11.84ITFVPYY245 pKa = 9.76TDD247 pKa = 4.54SEE249 pKa = 4.41TAYY252 pKa = 9.98TDD254 pKa = 3.57VLAGNLDD261 pKa = 3.83VAGVPASKK269 pKa = 9.11LTQAAGDD276 pKa = 3.78FGDD279 pKa = 3.52RR280 pKa = 11.84LYY282 pKa = 11.37SFEE285 pKa = 4.42APGISFLGLPLWNDD299 pKa = 3.1AYY301 pKa = 11.22DD302 pKa = 4.68DD303 pKa = 3.52IRR305 pKa = 11.84VRR307 pKa = 11.84QAISMAIDD315 pKa = 3.3RR316 pKa = 11.84EE317 pKa = 4.3AIIDD321 pKa = 3.89VIYY324 pKa = 10.72GGTYY328 pKa = 10.44DD329 pKa = 5.86AATAWTPAIEE339 pKa = 4.88PGTPAGICGEE349 pKa = 4.08YY350 pKa = 10.46CEE352 pKa = 4.98YY353 pKa = 11.19DD354 pKa = 3.59PEE356 pKa = 4.22AAKK359 pKa = 10.86ALLDD363 pKa = 3.71EE364 pKa = 5.52AGGFDD369 pKa = 4.13GSLEE373 pKa = 4.01IYY375 pKa = 10.43FPGGGGLDD383 pKa = 3.63PLYY386 pKa = 10.66EE387 pKa = 4.88AIANQLRR394 pKa = 11.84QNLGIEE400 pKa = 4.32ATAKK404 pKa = 10.5PSADD408 pKa = 2.68WAEE411 pKa = 4.02FLQNRR416 pKa = 11.84NDD418 pKa = 3.69EE419 pKa = 4.79NIDD422 pKa = 3.47GPFFSRR428 pKa = 11.84WGALYY433 pKa = 9.55PSQQATLRR441 pKa = 11.84VFFIEE446 pKa = 5.79GGGCTNCIPWYY457 pKa = 9.43SDD459 pKa = 3.02EE460 pKa = 4.58VADD463 pKa = 5.73AMAAADD469 pKa = 4.68ADD471 pKa = 4.07LSTDD475 pKa = 3.05GSAYY479 pKa = 10.62AEE481 pKa = 4.17VQKK484 pKa = 10.75LIQAEE489 pKa = 4.83FPAVPLFFEE498 pKa = 4.66KK499 pKa = 10.77YY500 pKa = 10.29SYY502 pKa = 9.07VTSEE506 pKa = 3.98KK507 pKa = 10.76VADD510 pKa = 4.1LVTSPVGNPTYY521 pKa = 10.59RR522 pKa = 11.84SIVLDD527 pKa = 3.96DD528 pKa = 3.64

Molecular weight: 56.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164BLB5|A0A164BLB5_9MICO DUF305 domain-containing protein OS=Microbacterium sp. TNHR37B OX=1775956 GN=AVP41_02624 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.64GRR40 pKa = 11.84TEE42 pKa = 4.13LSAA45 pKa = 4.86

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3221

0

3221

1083291

29

3542

336.3

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.738 ± 0.057

0.482 ± 0.009

6.301 ± 0.035

5.495 ± 0.037

3.063 ± 0.026

8.924 ± 0.036

1.984 ± 0.023

4.298 ± 0.035

1.755 ± 0.032

10.02 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.664 ± 0.018

1.782 ± 0.021

5.551 ± 0.029

2.626 ± 0.021

7.632 ± 0.055

5.647 ± 0.034

6.303 ± 0.053

9.157 ± 0.049

1.608 ± 0.019

1.97 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski