Pyrenochaeta sp. DS3sAY3a

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Cucurbitariaceae; Pyrenochaeta; unclassified Pyrenochaeta

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14984 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A178DWF5|A0A178DWF5_9PLEO Uncharacterized protein OS=Pyrenochaeta sp. DS3sAY3a OX=765867 GN=IQ07DRAFT_602113 PE=4 SV=1
MM1 pKa = 7.79IDD3 pKa = 3.72SDD5 pKa = 4.47SPCPYY10 pKa = 10.09EE11 pKa = 4.03EE12 pKa = 4.19QYY14 pKa = 11.22YY15 pKa = 10.61AALKK19 pKa = 10.44FMEE22 pKa = 5.38DD23 pKa = 3.67GEE25 pKa = 4.39EE26 pKa = 4.19EE27 pKa = 4.29KK28 pKa = 10.98CVAACKK34 pKa = 10.32RR35 pKa = 11.84NLLDD39 pKa = 3.49PTLPTLYY46 pKa = 10.03MIRR49 pKa = 11.84NCLLVACACEE59 pKa = 3.97TWDD62 pKa = 3.47EE63 pKa = 4.11GDD65 pKa = 3.59VYY67 pKa = 10.89RR68 pKa = 11.84RR69 pKa = 11.84AAEE72 pKa = 3.76EE73 pKa = 4.19MYY75 pKa = 10.61NGQFEE80 pKa = 4.51EE81 pKa = 4.52ATQKK85 pKa = 10.53CDD87 pKa = 3.66KK88 pKa = 10.56DD89 pKa = 3.53ALEE92 pKa = 4.24ALEE95 pKa = 4.2ILRR98 pKa = 11.84SNLDD102 pKa = 3.35EE103 pKa = 5.69CNDD106 pKa = 3.7SLDD109 pKa = 3.87ALLSEE114 pKa = 4.89EE115 pKa = 4.04TGIWISDD122 pKa = 3.65GYY124 pKa = 11.14AEE126 pKa = 4.33RR127 pKa = 11.84EE128 pKa = 3.92EE129 pKa = 4.51MLAEE133 pKa = 4.08MDD135 pKa = 3.32SDD137 pKa = 4.42EE138 pKa = 4.79FDD140 pKa = 3.81TYY142 pKa = 11.7DD143 pKa = 3.8SDD145 pKa = 4.14TEE147 pKa = 4.81LEE149 pKa = 4.17MEE151 pKa = 4.23EE152 pKa = 4.0QNAVRR157 pKa = 11.84EE158 pKa = 4.22VEE160 pKa = 4.24NNDD163 pKa = 2.9EE164 pKa = 4.15FAAEE168 pKa = 4.57SMTLPIRR175 pKa = 11.84EE176 pKa = 4.43TTSPTSAMPIITVNSSVEE194 pKa = 4.08NFTPTTSISKK204 pKa = 10.37

Molecular weight: 23.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A178DTQ4|A0A178DTQ4_9PLEO Kinase-like protein OS=Pyrenochaeta sp. DS3sAY3a OX=765867 GN=IQ07DRAFT_658852 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84RR3 pKa = 11.84SAPRR7 pKa = 11.84TTRR10 pKa = 11.84AHH12 pKa = 6.03HH13 pKa = 4.95TTAPRR18 pKa = 11.84TTRR21 pKa = 11.84TARR24 pKa = 11.84PSLKK28 pKa = 9.51TRR30 pKa = 11.84LLGPKK35 pKa = 9.55RR36 pKa = 11.84ATRR39 pKa = 11.84TTTRR43 pKa = 11.84RR44 pKa = 11.84TPHH47 pKa = 5.4TTTTTTTTTKK57 pKa = 7.04TTRR60 pKa = 11.84HH61 pKa = 6.09HH62 pKa = 6.41GVAGTAAPVHH72 pKa = 5.09HH73 pKa = 7.32HH74 pKa = 6.33KK75 pKa = 10.63RR76 pKa = 11.84HH77 pKa = 4.81ATMGDD82 pKa = 3.6KK83 pKa = 11.07VSGALMKK90 pKa = 10.77LRR92 pKa = 11.84GSLTRR97 pKa = 11.84RR98 pKa = 11.84PGLKK102 pKa = 10.1AAGTRR107 pKa = 11.84RR108 pKa = 11.84MHH110 pKa = 5.92GTDD113 pKa = 2.72GRR115 pKa = 11.84NARR118 pKa = 11.84RR119 pKa = 11.84VYY121 pKa = 10.89

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14984

0

14984

6812781

49

7183

454.7

50.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.658 ± 0.019

1.333 ± 0.009

5.543 ± 0.015

6.004 ± 0.02

3.838 ± 0.013

6.76 ± 0.018

2.457 ± 0.009

5.028 ± 0.015

4.884 ± 0.016

8.927 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.008

3.703 ± 0.01

6.015 ± 0.021

4.034 ± 0.015

6.029 ± 0.019

8.09 ± 0.021

6.06 ± 0.013

6.093 ± 0.014

1.569 ± 0.008

2.834 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski