Sediminitomix flava

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Sediminitomix

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5031 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A315ZA28|A0A315ZA28_9BACT 4Fe-4S binding protein OS=Sediminitomix flava OX=379075 GN=BC781_103382 PE=4 SV=1
MM1 pKa = 7.49LRR3 pKa = 11.84NKK5 pKa = 10.23QIVLPAITIFFLLLISFNYY24 pKa = 9.88PNTFNFNTSLDD35 pKa = 3.74RR36 pKa = 11.84DD37 pKa = 3.52ADD39 pKa = 4.49GITDD43 pKa = 3.43KK44 pKa = 11.1RR45 pKa = 11.84DD46 pKa = 3.51YY47 pKa = 11.04CPLEE51 pKa = 4.76AGFISTGGCPDD62 pKa = 3.34IDD64 pKa = 4.43LDD66 pKa = 4.61GVADD70 pKa = 3.93KK71 pKa = 10.39WDD73 pKa = 3.73KK74 pKa = 11.28CPEE77 pKa = 4.24LEE79 pKa = 5.02GSLDD83 pKa = 3.81SFGCPDD89 pKa = 3.58YY90 pKa = 11.63DD91 pKa = 4.17KK92 pKa = 11.42DD93 pKa = 4.21GVSDD97 pKa = 4.0TVDD100 pKa = 2.94QCPYY104 pKa = 11.32DD105 pKa = 3.81FGEE108 pKa = 4.74AKK110 pKa = 10.89NNGCPDD116 pKa = 4.03LDD118 pKa = 3.57QDD120 pKa = 4.66GVADD124 pKa = 5.29HH125 pKa = 7.09MDD127 pKa = 3.45DD128 pKa = 5.52CIDD131 pKa = 3.46EE132 pKa = 4.57KK133 pKa = 11.26GTVEE137 pKa = 4.56TNGCPDD143 pKa = 3.35TDD145 pKa = 3.69KK146 pKa = 11.7DD147 pKa = 4.09GVADD151 pKa = 5.06IYY153 pKa = 11.13DD154 pKa = 3.71KK155 pKa = 11.45CPTKK159 pKa = 10.61FGEE162 pKa = 4.15VKK164 pKa = 10.28YY165 pKa = 9.68WGCIDD170 pKa = 4.14SDD172 pKa = 3.89GDD174 pKa = 4.18LVGDD178 pKa = 5.4HH179 pKa = 7.13IDD181 pKa = 3.31EE182 pKa = 4.57CPYY185 pKa = 11.54DD186 pKa = 4.17KK187 pKa = 11.28GSTLNKK193 pKa = 9.27GCPVKK198 pKa = 10.84

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A315ZGC4|A0A315ZGC4_9BACT LuxR family two component transcriptional regulator OS=Sediminitomix flava OX=379075 GN=BC781_101732 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.55FFLTRR7 pKa = 11.84IAPISLLAFIAQMIFPWWGVIAVCSFIYY35 pKa = 10.03TLFMPPHH42 pKa = 6.62SGWQAFFMGFFALLLLWGSAASVIDD67 pKa = 3.55IQNEE71 pKa = 4.53GILSKK76 pKa = 10.39RR77 pKa = 11.84IAMILQLPRR86 pKa = 11.84SVYY89 pKa = 10.48LVALTAFLGGLIAGLSSLSGFHH111 pKa = 6.21FRR113 pKa = 11.84RR114 pKa = 11.84MLSII118 pKa = 4.23

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5031

0

5031

1924432

24

6844

382.5

43.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.205 ± 0.038

0.755 ± 0.013

5.762 ± 0.04

7.427 ± 0.033

5.195 ± 0.026

6.542 ± 0.039

1.876 ± 0.019

7.238 ± 0.028

7.089 ± 0.06

9.394 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.019

5.52 ± 0.03

3.369 ± 0.018

3.686 ± 0.022

3.504 ± 0.026

6.943 ± 0.041

5.479 ± 0.044

6.263 ± 0.031

1.227 ± 0.014

4.233 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski