Dickeya phage Dagda_B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Aarhusvirus; unclassified Aarhusvirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385IFH9|A0A385IFH9_9CAUD Uncharacterized protein OS=Dickeya phage Dagda_B1 OX=2320189 PE=4 SV=1
MM1 pKa = 7.24TDD3 pKa = 2.96IYY5 pKa = 11.38AEE7 pKa = 4.12YY8 pKa = 9.99GVTGAVMSEE17 pKa = 3.76PSEE20 pKa = 4.4GYY22 pKa = 9.53NEE24 pKa = 3.96QMIAQPVDD32 pKa = 3.26VRR34 pKa = 11.84DD35 pKa = 3.98GDD37 pKa = 4.09DD38 pKa = 3.95SITVEE43 pKa = 3.97SEE45 pKa = 3.87EE46 pKa = 4.11FEE48 pKa = 4.28EE49 pKa = 5.04ASSEE53 pKa = 3.92EE54 pKa = 4.17LPEE57 pKa = 4.71GEE59 pKa = 4.37EE60 pKa = 3.89QGEE63 pKa = 4.28EE64 pKa = 3.95QSGEE68 pKa = 4.05EE69 pKa = 4.13SGEE72 pKa = 4.14EE73 pKa = 3.66AGEE76 pKa = 3.88AAEE79 pKa = 4.69FEE81 pKa = 4.55AVGDD85 pKa = 3.93VPEE88 pKa = 4.33EE89 pKa = 4.0LVTVTTRR96 pKa = 11.84IAEE99 pKa = 4.23SEE101 pKa = 4.49SAFNDD106 pKa = 3.47MVADD110 pKa = 4.46AAARR114 pKa = 11.84GLDD117 pKa = 3.4QEE119 pKa = 4.47SFAIISAEE127 pKa = 3.92YY128 pKa = 9.0EE129 pKa = 4.19AEE131 pKa = 4.42GEE133 pKa = 4.23LSPKK137 pKa = 10.37SYY139 pKa = 10.51EE140 pKa = 3.93ALAKK144 pKa = 10.39VGYY147 pKa = 9.13SKK149 pKa = 11.31NFIDD153 pKa = 5.67SFIAGQEE160 pKa = 4.36AIGSQYY166 pKa = 10.28MSAIQAYY173 pKa = 9.85AGGEE177 pKa = 4.02AKK179 pKa = 10.3FQAIFAHH186 pKa = 6.37LQASSPEE193 pKa = 4.08SAEE196 pKa = 3.87ALEE199 pKa = 4.14NAIGQRR205 pKa = 11.84DD206 pKa = 3.77LKK208 pKa = 10.67AVKK211 pKa = 10.48GIINLAASSVRR222 pKa = 11.84KK223 pKa = 9.76EE224 pKa = 3.89FGKK227 pKa = 9.7PAEE230 pKa = 4.13RR231 pKa = 11.84TLSKK235 pKa = 10.38RR236 pKa = 11.84ATPAKK241 pKa = 9.08VTAPKK246 pKa = 10.39AAGFEE251 pKa = 4.65SQAEE255 pKa = 4.24MVKK258 pKa = 10.68AMKK261 pKa = 10.08DD262 pKa = 3.28PKK264 pKa = 10.08YY265 pKa = 10.96GRR267 pKa = 11.84DD268 pKa = 3.13AAYY271 pKa = 8.85TRR273 pKa = 11.84SVEE276 pKa = 4.69LKK278 pKa = 10.21VLNAKK283 pKa = 9.48FF284 pKa = 3.3

Molecular weight: 30.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385IFE2|A0A385IFE2_9CAUD DNA-directed RNA polymerase OS=Dickeya phage Dagda_B1 OX=2320189 PE=3 SV=1
MM1 pKa = 6.03VTAWDD6 pKa = 4.0TMSGTLRR13 pKa = 11.84LSWKK17 pKa = 9.85ALRR20 pKa = 11.84AKK22 pKa = 9.42MHH24 pKa = 5.84GRR26 pKa = 11.84PLCWRR31 pKa = 11.84NKK33 pKa = 6.06MTSKK37 pKa = 10.97SNSQNKK43 pKa = 10.01AGFIKK48 pKa = 10.0RR49 pKa = 11.84TWQRR53 pKa = 11.84TKK55 pKa = 10.99LIPLGLLMIYY65 pKa = 10.29VSLVTGCASGSQPVRR80 pKa = 11.84PLSPQVQVDD89 pKa = 3.65QALMVTPNFQEE100 pKa = 4.12TLLKK104 pKa = 10.16YY105 pKa = 10.77LSVNPDD111 pKa = 2.89VPMSGLNDD119 pKa = 3.6SKK121 pKa = 11.31RR122 pKa = 11.84PP123 pKa = 3.6

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12766

30

1320

240.9

26.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.345 ± 0.459

0.995 ± 0.167

6.032 ± 0.213

6.729 ± 0.379

3.924 ± 0.231

7.418 ± 0.373

1.864 ± 0.192

5.233 ± 0.253

6.556 ± 0.308

7.966 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.039 ± 0.168

4.692 ± 0.245

3.838 ± 0.183

4.206 ± 0.369

5.115 ± 0.155

6.431 ± 0.296

5.468 ± 0.272

6.682 ± 0.273

1.433 ± 0.164

3.031 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski