Amino acid dipepetide frequency for Temperate phage phiNIH1.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.728AlaAla: 4.728 ± 1.646
0.438AlaCys: 0.438 ± 0.231
4.816AlaAsp: 4.816 ± 0.597
6.042AlaGlu: 6.042 ± 0.812
2.277AlaPhe: 2.277 ± 0.445
3.59AlaGly: 3.59 ± 0.726
0.613AlaHis: 0.613 ± 0.175
5.779AlaIle: 5.779 ± 0.574
6.654AlaLys: 6.654 ± 0.649
7.355AlaLeu: 7.355 ± 0.844
1.839AlaMet: 1.839 ± 0.466
4.203AlaAsn: 4.203 ± 0.588
1.751AlaPro: 1.751 ± 0.291
3.677AlaGln: 3.677 ± 0.628
2.889AlaArg: 2.889 ± 0.611
4.553AlaSer: 4.553 ± 1.027
4.115AlaThr: 4.115 ± 0.776
4.29AlaVal: 4.29 ± 0.593
0.788AlaTrp: 0.788 ± 0.275
2.452AlaTyr: 2.452 ± 0.463
0.0AlaXaa: 0.0 ± 0.0
Cys
0.263CysAla: 0.263 ± 0.154
0.088CysCys: 0.088 ± 0.079
0.35CysAsp: 0.35 ± 0.135
0.438CysGlu: 0.438 ± 0.218
0.613CysPhe: 0.613 ± 0.243
0.263CysGly: 0.263 ± 0.175
0.175CysHis: 0.175 ± 0.172
0.263CysIle: 0.263 ± 0.199
0.788CysLys: 0.788 ± 0.231
1.051CysLeu: 1.051 ± 0.269
0.263CysMet: 0.263 ± 0.134
0.175CysAsn: 0.175 ± 0.127
0.175CysPro: 0.175 ± 0.112
0.35CysGln: 0.35 ± 0.177
0.35CysArg: 0.35 ± 0.159
0.088CysSer: 0.088 ± 0.083
0.088CysThr: 0.088 ± 0.078
0.613CysVal: 0.613 ± 0.234
0.088CysTrp: 0.088 ± 0.086
0.35CysTyr: 0.35 ± 0.175
0.0CysXaa: 0.0 ± 0.0
Asp
4.553AspAla: 4.553 ± 0.726
0.263AspCys: 0.263 ± 0.158
4.991AspAsp: 4.991 ± 0.883
4.903AspGlu: 4.903 ± 0.683
3.327AspPhe: 3.327 ± 0.441
6.654AspGly: 6.654 ± 0.62
1.051AspHis: 1.051 ± 0.237
3.59AspIle: 3.59 ± 0.525
6.042AspLys: 6.042 ± 0.72
7.18AspLeu: 7.18 ± 0.945
1.576AspMet: 1.576 ± 0.351
4.816AspAsn: 4.816 ± 0.652
1.488AspPro: 1.488 ± 0.368
1.926AspGln: 1.926 ± 0.474
2.364AspArg: 2.364 ± 0.582
2.977AspSer: 2.977 ± 0.525
3.502AspThr: 3.502 ± 0.491
5.253AspVal: 5.253 ± 0.697
0.7AspTrp: 0.7 ± 0.213
3.24AspTyr: 3.24 ± 0.661
0.0AspXaa: 0.0 ± 0.0
Glu
5.604GluAla: 5.604 ± 0.674
0.438GluCys: 0.438 ± 0.173
3.94GluAsp: 3.94 ± 0.651
6.042GluGlu: 6.042 ± 0.86
3.065GluPhe: 3.065 ± 0.609
2.802GluGly: 2.802 ± 0.498
0.876GluHis: 0.876 ± 0.269
7.092GluIle: 7.092 ± 1.02
5.691GluLys: 5.691 ± 0.752
8.318GluLeu: 8.318 ± 0.67
2.014GluMet: 2.014 ± 0.344
3.677GluAsn: 3.677 ± 0.619
1.138GluPro: 1.138 ± 0.333
2.539GluGln: 2.539 ± 0.574
3.677GluArg: 3.677 ± 0.668
4.203GluSer: 4.203 ± 0.619
4.728GluThr: 4.728 ± 0.598
4.465GluVal: 4.465 ± 0.74
0.525GluTrp: 0.525 ± 0.179
3.327GluTyr: 3.327 ± 0.686
0.0GluXaa: 0.0 ± 0.0
Phe
2.539PheAla: 2.539 ± 0.464
0.525PheCys: 0.525 ± 0.253
4.028PheAsp: 4.028 ± 0.522
2.889PheGlu: 2.889 ± 0.625
1.138PhePhe: 1.138 ± 0.398
2.802PheGly: 2.802 ± 0.548
0.263PheHis: 0.263 ± 0.14
2.977PheIle: 2.977 ± 0.764
3.415PheLys: 3.415 ± 0.471
2.277PheLeu: 2.277 ± 0.501
0.613PheMet: 0.613 ± 0.22
2.802PheAsn: 2.802 ± 0.479
0.35PhePro: 0.35 ± 0.186
0.876PheGln: 0.876 ± 0.258
2.101PheArg: 2.101 ± 0.414
2.364PheSer: 2.364 ± 0.524
2.364PheThr: 2.364 ± 0.443
2.802PheVal: 2.802 ± 0.546
0.175PheTrp: 0.175 ± 0.137
1.488PheTyr: 1.488 ± 0.316
0.0PheXaa: 0.0 ± 0.0
Gly
4.641GlyAla: 4.641 ± 0.829
0.788GlyCys: 0.788 ± 0.271
2.977GlyAsp: 2.977 ± 0.445
4.115GlyGlu: 4.115 ± 0.534
2.977GlyPhe: 2.977 ± 0.497
2.977GlyGly: 2.977 ± 0.5
1.576GlyHis: 1.576 ± 0.316
5.166GlyIle: 5.166 ± 0.732
4.728GlyLys: 4.728 ± 0.478
4.29GlyLeu: 4.29 ± 0.813
1.051GlyMet: 1.051 ± 0.262
3.415GlyAsn: 3.415 ± 0.669
0.963GlyPro: 0.963 ± 0.37
2.189GlyGln: 2.189 ± 0.515
2.277GlyArg: 2.277 ± 0.436
2.889GlySer: 2.889 ± 0.425
3.415GlyThr: 3.415 ± 0.5
5.954GlyVal: 5.954 ± 0.953
0.788GlyTrp: 0.788 ± 0.336
1.839GlyTyr: 1.839 ± 0.499
0.0GlyXaa: 0.0 ± 0.0
His
0.876HisAla: 0.876 ± 0.279
0.088HisCys: 0.088 ± 0.086
0.7HisAsp: 0.7 ± 0.224
0.963HisGlu: 0.963 ± 0.3
0.963HisPhe: 0.963 ± 0.22
1.313HisGly: 1.313 ± 0.295
0.175HisHis: 0.175 ± 0.118
0.788HisIle: 0.788 ± 0.242
0.788HisLys: 0.788 ± 0.24
1.576HisLeu: 1.576 ± 0.388
0.175HisMet: 0.175 ± 0.128
0.788HisAsn: 0.788 ± 0.22
0.438HisPro: 0.438 ± 0.181
0.613HisGln: 0.613 ± 0.23
0.613HisArg: 0.613 ± 0.194
1.051HisSer: 1.051 ± 0.397
1.313HisThr: 1.313 ± 0.288
0.788HisVal: 0.788 ± 0.293
0.088HisTrp: 0.088 ± 0.087
0.613HisTyr: 0.613 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
5.341IleAla: 5.341 ± 0.742
0.175IleCys: 0.175 ± 0.116
7.267IleAsp: 7.267 ± 0.822
5.516IleGlu: 5.516 ± 0.698
2.101IlePhe: 2.101 ± 0.53
3.415IleGly: 3.415 ± 0.392
0.613IleHis: 0.613 ± 0.262
3.853IleIle: 3.853 ± 0.635
7.793IleLys: 7.793 ± 0.87
4.465IleLeu: 4.465 ± 0.522
1.226IleMet: 1.226 ± 0.371
5.078IleAsn: 5.078 ± 0.652
1.664IlePro: 1.664 ± 0.333
1.488IleGln: 1.488 ± 0.351
1.751IleArg: 1.751 ± 0.432
5.341IleSer: 5.341 ± 0.798
5.166IleThr: 5.166 ± 0.545
4.203IleVal: 4.203 ± 0.685
0.7IleTrp: 0.7 ± 0.272
3.065IleTyr: 3.065 ± 0.54
0.0IleXaa: 0.0 ± 0.0
Lys
7.53LysAla: 7.53 ± 0.884
0.525LysCys: 0.525 ± 0.201
5.691LysAsp: 5.691 ± 0.716
6.83LysGlu: 6.83 ± 0.948
1.926LysPhe: 1.926 ± 0.365
4.115LysGly: 4.115 ± 0.528
1.051LysHis: 1.051 ± 0.267
6.042LysIle: 6.042 ± 0.706
8.581LysLys: 8.581 ± 0.917
6.654LysLeu: 6.654 ± 0.894
2.802LysMet: 2.802 ± 0.444
5.341LysAsn: 5.341 ± 0.522
3.065LysPro: 3.065 ± 0.642
3.677LysGln: 3.677 ± 0.598
3.59LysArg: 3.59 ± 0.544
4.991LysSer: 4.991 ± 0.59
5.516LysThr: 5.516 ± 0.645
6.917LysVal: 6.917 ± 0.746
1.401LysTrp: 1.401 ± 0.288
3.853LysTyr: 3.853 ± 0.51
0.0LysXaa: 0.0 ± 0.0
Leu
6.654LeuAla: 6.654 ± 0.846
0.263LeuCys: 0.263 ± 0.158
6.917LeuAsp: 6.917 ± 1.039
7.092LeuGlu: 7.092 ± 0.676
3.327LeuPhe: 3.327 ± 0.876
4.816LeuGly: 4.816 ± 0.568
0.788LeuHis: 0.788 ± 0.278
5.253LeuIle: 5.253 ± 0.753
8.318LeuLys: 8.318 ± 0.737
5.866LeuLeu: 5.866 ± 0.898
1.488LeuMet: 1.488 ± 0.409
5.166LeuAsn: 5.166 ± 0.542
2.014LeuPro: 2.014 ± 0.469
2.802LeuGln: 2.802 ± 0.51
4.028LeuArg: 4.028 ± 0.593
5.516LeuSer: 5.516 ± 0.611
4.203LeuThr: 4.203 ± 0.477
4.115LeuVal: 4.115 ± 0.589
0.788LeuTrp: 0.788 ± 0.218
3.327LeuTyr: 3.327 ± 0.608
0.0LeuXaa: 0.0 ± 0.0
Met
1.839MetAla: 1.839 ± 0.418
0.088MetCys: 0.088 ± 0.094
1.138MetAsp: 1.138 ± 0.369
1.488MetGlu: 1.488 ± 0.377
0.876MetPhe: 0.876 ± 0.345
1.313MetGly: 1.313 ± 0.385
0.35MetHis: 0.35 ± 0.167
1.313MetIle: 1.313 ± 0.322
1.401MetLys: 1.401 ± 0.364
1.926MetLeu: 1.926 ± 0.54
0.438MetMet: 0.438 ± 0.197
1.313MetAsn: 1.313 ± 0.302
0.963MetPro: 0.963 ± 0.253
1.401MetGln: 1.401 ± 0.49
2.014MetArg: 2.014 ± 0.336
1.751MetSer: 1.751 ± 0.499
1.839MetThr: 1.839 ± 0.365
0.613MetVal: 0.613 ± 0.266
0.175MetTrp: 0.175 ± 0.117
0.438MetTyr: 0.438 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
3.94AsnAla: 3.94 ± 0.71
0.525AsnCys: 0.525 ± 0.211
3.677AsnAsp: 3.677 ± 0.487
3.327AsnGlu: 3.327 ± 0.528
2.014AsnPhe: 2.014 ± 0.455
3.94AsnGly: 3.94 ± 0.57
1.051AsnHis: 1.051 ± 0.288
3.765AsnIle: 3.765 ± 0.562
4.903AsnLys: 4.903 ± 0.646
4.816AsnLeu: 4.816 ± 0.477
1.226AsnMet: 1.226 ± 0.373
3.152AsnAsn: 3.152 ± 0.447
2.364AsnPro: 2.364 ± 0.461
2.977AsnGln: 2.977 ± 0.568
2.539AsnArg: 2.539 ± 0.4
3.853AsnSer: 3.853 ± 0.715
2.977AsnThr: 2.977 ± 0.587
2.802AsnVal: 2.802 ± 0.452
0.963AsnTrp: 0.963 ± 0.339
1.839AsnTyr: 1.839 ± 0.418
0.0AsnXaa: 0.0 ± 0.0
Pro
1.138ProAla: 1.138 ± 0.269
0.175ProCys: 0.175 ± 0.108
2.101ProAsp: 2.101 ± 0.54
2.189ProGlu: 2.189 ± 0.384
1.488ProPhe: 1.488 ± 0.474
1.051ProGly: 1.051 ± 0.284
0.788ProHis: 0.788 ± 0.263
1.401ProIle: 1.401 ± 0.41
2.452ProLys: 2.452 ± 0.51
2.189ProLeu: 2.189 ± 0.459
0.613ProMet: 0.613 ± 0.22
1.401ProAsn: 1.401 ± 0.317
0.263ProPro: 0.263 ± 0.148
1.138ProGln: 1.138 ± 0.329
0.963ProArg: 0.963 ± 0.305
1.664ProSer: 1.664 ± 0.385
1.488ProThr: 1.488 ± 0.429
2.189ProVal: 2.189 ± 0.391
0.175ProTrp: 0.175 ± 0.164
1.401ProTyr: 1.401 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
2.802GlnAla: 2.802 ± 0.564
0.263GlnCys: 0.263 ± 0.135
1.664GlnAsp: 1.664 ± 0.512
3.24GlnGlu: 3.24 ± 0.552
1.226GlnPhe: 1.226 ± 0.258
3.065GlnGly: 3.065 ± 0.562
0.263GlnHis: 0.263 ± 0.139
2.802GlnIle: 2.802 ± 0.515
3.94GlnLys: 3.94 ± 0.516
3.065GlnLeu: 3.065 ± 0.564
0.963GlnMet: 0.963 ± 0.283
2.014GlnAsn: 2.014 ± 0.574
1.138GlnPro: 1.138 ± 0.3
1.664GlnGln: 1.664 ± 0.597
2.014GlnArg: 2.014 ± 0.456
2.977GlnSer: 2.977 ± 0.45
2.889GlnThr: 2.889 ± 0.601
1.751GlnVal: 1.751 ± 0.4
0.438GlnTrp: 0.438 ± 0.154
0.876GlnTyr: 0.876 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
2.802ArgAla: 2.802 ± 0.496
0.438ArgCys: 0.438 ± 0.17
2.364ArgAsp: 2.364 ± 0.474
2.627ArgGlu: 2.627 ± 0.523
1.576ArgPhe: 1.576 ± 0.313
2.101ArgGly: 2.101 ± 0.483
1.051ArgHis: 1.051 ± 0.379
3.24ArgIle: 3.24 ± 0.418
3.415ArgLys: 3.415 ± 0.529
3.677ArgLeu: 3.677 ± 0.691
0.876ArgMet: 0.876 ± 0.219
2.101ArgAsn: 2.101 ± 0.352
0.7ArgPro: 0.7 ± 0.276
2.101ArgGln: 2.101 ± 0.443
2.452ArgArg: 2.452 ± 0.501
1.664ArgSer: 1.664 ± 0.379
2.539ArgThr: 2.539 ± 0.542
2.627ArgVal: 2.627 ± 0.483
0.525ArgTrp: 0.525 ± 0.202
2.101ArgTyr: 2.101 ± 0.477
0.0ArgXaa: 0.0 ± 0.0
Ser
4.465SerAla: 4.465 ± 1.007
0.438SerCys: 0.438 ± 0.188
4.378SerAsp: 4.378 ± 0.716
4.378SerGlu: 4.378 ± 0.559
2.977SerPhe: 2.977 ± 0.491
3.94SerGly: 3.94 ± 0.553
1.051SerHis: 1.051 ± 0.303
4.29SerIle: 4.29 ± 0.571
5.691SerLys: 5.691 ± 0.631
4.903SerLeu: 4.903 ± 0.706
1.226SerMet: 1.226 ± 0.307
3.065SerAsn: 3.065 ± 0.595
1.488SerPro: 1.488 ± 0.366
2.889SerGln: 2.889 ± 0.44
1.926SerArg: 1.926 ± 0.397
3.152SerSer: 3.152 ± 0.668
2.714SerThr: 2.714 ± 0.862
3.59SerVal: 3.59 ± 0.555
1.313SerTrp: 1.313 ± 0.332
1.839SerTyr: 1.839 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
5.341ThrAla: 5.341 ± 0.923
0.263ThrCys: 0.263 ± 0.156
4.553ThrAsp: 4.553 ± 0.654
3.677ThrGlu: 3.677 ± 0.566
2.627ThrPhe: 2.627 ± 0.486
4.028ThrGly: 4.028 ± 0.598
0.876ThrHis: 0.876 ± 0.234
4.465ThrIle: 4.465 ± 0.568
5.253ThrLys: 5.253 ± 0.561
4.991ThrLeu: 4.991 ± 0.666
1.488ThrMet: 1.488 ± 0.507
2.802ThrAsn: 2.802 ± 0.489
2.189ThrPro: 2.189 ± 0.386
2.189ThrGln: 2.189 ± 0.391
1.926ThrArg: 1.926 ± 0.452
3.327ThrSer: 3.327 ± 0.5
4.816ThrThr: 4.816 ± 0.696
4.378ThrVal: 4.378 ± 0.545
0.525ThrTrp: 0.525 ± 0.216
1.839ThrTyr: 1.839 ± 0.513
0.0ThrXaa: 0.0 ± 0.0
Val
4.641ValAla: 4.641 ± 0.568
0.35ValCys: 0.35 ± 0.193
4.991ValAsp: 4.991 ± 0.551
5.691ValGlu: 5.691 ± 0.88
2.364ValPhe: 2.364 ± 0.558
4.378ValGly: 4.378 ± 0.73
0.876ValHis: 0.876 ± 0.292
4.728ValIle: 4.728 ± 0.496
5.166ValLys: 5.166 ± 0.713
4.203ValLeu: 4.203 ± 0.719
2.014ValMet: 2.014 ± 0.395
3.415ValAsn: 3.415 ± 0.44
1.926ValPro: 1.926 ± 0.425
1.926ValGln: 1.926 ± 0.423
2.014ValArg: 2.014 ± 0.321
4.378ValSer: 4.378 ± 0.522
4.028ValThr: 4.028 ± 0.575
4.028ValVal: 4.028 ± 0.349
0.613ValTrp: 0.613 ± 0.217
2.452ValTyr: 2.452 ± 0.62
0.0ValXaa: 0.0 ± 0.0
Trp
0.525TrpAla: 0.525 ± 0.198
0.175TrpCys: 0.175 ± 0.121
0.963TrpAsp: 0.963 ± 0.237
0.525TrpGlu: 0.525 ± 0.187
0.613TrpPhe: 0.613 ± 0.249
0.788TrpGly: 0.788 ± 0.351
0.088TrpHis: 0.088 ± 0.08
0.876TrpIle: 0.876 ± 0.239
1.226TrpLys: 1.226 ± 0.284
0.963TrpLeu: 0.963 ± 0.28
0.175TrpMet: 0.175 ± 0.128
0.438TrpAsn: 0.438 ± 0.163
0.35TrpPro: 0.35 ± 0.188
0.7TrpGln: 0.7 ± 0.218
0.525TrpArg: 0.525 ± 0.246
0.613TrpSer: 0.613 ± 0.2
0.7TrpThr: 0.7 ± 0.328
0.7TrpVal: 0.7 ± 0.232
0.0TrpTrp: 0.0 ± 0.0
0.263TrpTyr: 0.263 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.627TyrAla: 2.627 ± 0.494
0.525TyrCys: 0.525 ± 0.215
2.889TyrAsp: 2.889 ± 0.411
2.189TyrGlu: 2.189 ± 0.364
1.313TyrPhe: 1.313 ± 0.449
1.664TyrGly: 1.664 ± 0.324
1.138TyrHis: 1.138 ± 0.334
2.627TyrIle: 2.627 ± 0.397
3.853TyrLys: 3.853 ± 0.624
2.977TyrLeu: 2.977 ± 0.573
0.525TyrMet: 0.525 ± 0.201
1.488TyrAsn: 1.488 ± 0.354
1.839TyrPro: 1.839 ± 0.452
1.926TyrGln: 1.926 ± 0.386
0.963TyrArg: 0.963 ± 0.286
2.364TyrSer: 2.364 ± 0.455
3.152TyrThr: 3.152 ± 0.669
2.101TyrVal: 2.101 ± 0.476
0.438TyrTrp: 0.438 ± 0.184
1.313TyrTyr: 1.313 ± 0.364
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11422 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski