Geosporobacter subterraneus DSM 17957

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Geosporobacter; Geosporobacter subterraneus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6QJ98|A0A1M6QJ98_9CLOT Dihydroxyacetone kinase DhaL subunit OS=Geosporobacter subterraneus DSM 17957 OX=1121919 GN=SAMN02745975_03860 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.38GVTIFGNFTVLEE14 pKa = 4.16TFMFNDD20 pKa = 3.8LQIDD24 pKa = 3.44KK25 pKa = 11.07GFDD28 pKa = 3.03EE29 pKa = 5.26RR30 pKa = 11.84LDD32 pKa = 3.0IFYY35 pKa = 10.65RR36 pKa = 11.84VNLYY40 pKa = 9.96IEE42 pKa = 4.36NLEE45 pKa = 4.69DD46 pKa = 5.93DD47 pKa = 4.3INDD50 pKa = 3.89TFIDD54 pKa = 4.11CYY56 pKa = 10.67YY57 pKa = 10.93RR58 pKa = 11.84SINDD62 pKa = 3.21LMEE65 pKa = 5.67DD66 pKa = 3.75IKK68 pKa = 11.08CYY70 pKa = 10.66SINQTT75 pKa = 3.41

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6QKW3|A0A1M6QKW3_9CLOT Uncharacterized protein (Fragment) OS=Geosporobacter subterraneus DSM 17957 OX=1121919 GN=SAMN02745975_03868 PE=4 SV=1
MM1 pKa = 7.3NEE3 pKa = 3.56SMLWLLRR10 pKa = 11.84DD11 pKa = 3.21TGKK14 pKa = 10.76AKK16 pKa = 10.33KK17 pKa = 10.07QGMNAIKK24 pKa = 9.94EE25 pKa = 4.22RR26 pKa = 11.84QRR28 pKa = 11.84TRR30 pKa = 11.84LAEE33 pKa = 3.76IVAFARR39 pKa = 11.84GNSPYY44 pKa = 10.23YY45 pKa = 10.2RR46 pKa = 11.84EE47 pKa = 4.42LYY49 pKa = 10.07KK50 pKa = 10.67DD51 pKa = 3.39LPEE54 pKa = 6.19RR55 pKa = 11.84IEE57 pKa = 4.36DD58 pKa = 3.84TTLLPITSKK67 pKa = 10.15MKK69 pKa = 10.62LMPRR73 pKa = 11.84FNDD76 pKa = 2.69WCTDD80 pKa = 3.33RR81 pKa = 11.84NVTFDD86 pKa = 3.43KK87 pKa = 11.21VSAFVDD93 pKa = 3.58NPDD96 pKa = 4.32LIGKK100 pKa = 9.48RR101 pKa = 11.84FLEE104 pKa = 4.86KK105 pKa = 10.3YY106 pKa = 9.13TITTTSGITRR116 pKa = 11.84TRR118 pKa = 11.84GIFLLNDD125 pKa = 3.32RR126 pKa = 11.84SLAVASSIAFRR137 pKa = 11.84MMSSWIGKK145 pKa = 7.47WDD147 pKa = 3.55VVRR150 pKa = 11.84IIPKK154 pKa = 9.43PVTAMPPPRR163 pKa = 11.84LLTTILIHH171 pKa = 6.63GARR174 pKa = 11.84QQRR177 pKa = 11.84LARR180 pKa = 11.84AGSCRR185 pKa = 11.84RR186 pKa = 11.84QLPTMAPGEE195 pKa = 4.11WSHH198 pKa = 5.99TVLVATSPTGYY209 pKa = 8.66SQSYY213 pKa = 7.12ATTWAIVSCCGLPLARR229 pKa = 11.84VPGINIIQILQTTPTNLRR247 pKa = 11.84VRR249 pKa = 11.84LRR251 pKa = 11.84PATGAAA257 pKa = 3.55

Molecular weight: 28.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3760

0

3760

1097426

39

3284

291.9

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.154 ± 0.041

1.03 ± 0.014

5.125 ± 0.028

7.581 ± 0.044

4.052 ± 0.028

7.105 ± 0.041

1.889 ± 0.019

8.824 ± 0.04

7.39 ± 0.035

9.543 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.98 ± 0.02

4.41 ± 0.028

3.427 ± 0.025

3.508 ± 0.026

4.405 ± 0.031

5.433 ± 0.031

4.968 ± 0.031

6.754 ± 0.037

0.764 ± 0.015

3.659 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski