Rouxiella badensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Rouxiella

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0WB26|A0A1X0WB26_9GAMM Uncharacterized protein OS=Rouxiella badensis OX=1646377 GN=BS640_18890 PE=4 SV=1
MM1 pKa = 7.55SMTYY5 pKa = 10.17TDD7 pKa = 4.01VKK9 pKa = 10.78NPSYY13 pKa = 10.93KK14 pKa = 10.67NSDD17 pKa = 3.28STIIDD22 pKa = 3.85CEE24 pKa = 4.46VKK26 pKa = 10.47FDD28 pKa = 4.62GFDD31 pKa = 4.41DD32 pKa = 3.99YY33 pKa = 12.01LQFTATATDD42 pKa = 3.51TTDD45 pKa = 3.26YY46 pKa = 11.38GVDD49 pKa = 3.0IFNACVAGTYY59 pKa = 8.4GTVAAYY65 pKa = 10.05VATKK69 pKa = 10.11TNAEE73 pKa = 3.99AVATAKK79 pKa = 10.37KK80 pKa = 10.14AALLQSINNTTQLWQTQLALGIITDD105 pKa = 3.86ADD107 pKa = 3.9KK108 pKa = 8.5TTLTAWMKK116 pKa = 9.9YY117 pKa = 7.73AQEE120 pKa = 4.02VQAIDD125 pKa = 3.47TTATSITWPEE135 pKa = 4.02EE136 pKa = 3.63PTTLTTTTTEE146 pKa = 4.01ASSDD150 pKa = 3.59TATSTDD156 pKa = 3.28TTTTTTTEE164 pKa = 4.07TAA166 pKa = 3.45

Molecular weight: 17.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0W9L1|A0A1X0W9L1_9GAMM DNA polymerase I OS=Rouxiella badensis OX=1646377 GN=polA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.55GRR39 pKa = 11.84TRR41 pKa = 11.84LSASKK46 pKa = 10.74

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4260

0

4260

1385808

23

5251

325.3

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.799 ± 0.043

0.998 ± 0.012

5.063 ± 0.026

5.547 ± 0.04

3.921 ± 0.024

7.482 ± 0.034

2.252 ± 0.019

5.797 ± 0.028

4.289 ± 0.031

11.039 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.017

3.679 ± 0.025

4.559 ± 0.025

4.548 ± 0.027

5.462 ± 0.034

6.317 ± 0.028

5.281 ± 0.021

7.129 ± 0.025

1.383 ± 0.016

2.795 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski