Eupatorium vein clearing virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Caulimovirus; unclassified Caulimovirus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2D1N0|B2D1N0_9VIRU Aspartic protease OS=Eupatorium vein clearing virus OX=515444 PE=3 SV=1
MM1 pKa = 7.22LTDD4 pKa = 3.4IQKK7 pKa = 8.36VTNLFFDD14 pKa = 3.47SWKK17 pKa = 10.19EE18 pKa = 4.12GEE20 pKa = 4.02RR21 pKa = 11.84HH22 pKa = 5.07EE23 pKa = 5.41AFTSEE28 pKa = 3.73IAKK31 pKa = 10.23IMSLPEE37 pKa = 3.7EE38 pKa = 4.44EE39 pKa = 4.54ANLMLRR45 pKa = 11.84NNSHH49 pKa = 6.64LRR51 pKa = 11.84GIFQLDD57 pKa = 3.46DD58 pKa = 3.93PQNLFQLTEE67 pKa = 4.12LEE69 pKa = 4.62EE70 pKa = 4.4EE71 pKa = 4.38QSSPVYY77 pKa = 10.63DD78 pKa = 4.64SAPEE82 pKa = 3.66WDD84 pKa = 5.05EE85 pKa = 4.52YY86 pKa = 11.64DD87 pKa = 3.68DD88 pKa = 6.43DD89 pKa = 5.47YY90 pKa = 11.94ILVITNNAHH99 pKa = 7.19SDD101 pKa = 3.46DD102 pKa = 4.84GYY104 pKa = 10.26NTDD107 pKa = 3.48DD108 pKa = 4.67TINVTIPSDD117 pKa = 3.37TDD119 pKa = 3.32EE120 pKa = 4.64EE121 pKa = 4.38EE122 pKa = 4.43EE123 pKa = 4.63EE124 pKa = 4.25INLGNLRR131 pKa = 11.84PEE133 pKa = 4.25IFQDD137 pKa = 3.85DD138 pKa = 4.16EE139 pKa = 4.73ASSGVFNEE147 pKa = 4.61SGLSGGPSTSRR158 pKa = 11.84PQPRR162 pKa = 11.84PNMGDD167 pKa = 3.51PMRR170 pKa = 11.84LKK172 pKa = 10.29PDD174 pKa = 2.89TGTQVLNLDD183 pKa = 4.05CTTSFSGRR191 pKa = 11.84RR192 pKa = 11.84ALIEE196 pKa = 3.93LWKK199 pKa = 10.78KK200 pKa = 10.62EE201 pKa = 3.79MDD203 pKa = 4.26IILLTGKK210 pKa = 9.58IRR212 pKa = 11.84TAEE215 pKa = 4.04EE216 pKa = 4.59LIMLVDD222 pKa = 3.98YY223 pKa = 8.32KK224 pKa = 11.01TAGNVNAAIKK234 pKa = 9.99GYY236 pKa = 9.25SWNRR240 pKa = 11.84QFSPADD246 pKa = 3.38LLEE249 pKa = 4.24AVRR252 pKa = 11.84KK253 pKa = 9.47VLYY256 pKa = 9.76TVFLGEE262 pKa = 4.07DD263 pKa = 3.34HH264 pKa = 6.67ATQEE268 pKa = 4.27ALEE271 pKa = 4.31VAVRR275 pKa = 11.84IANAKK280 pKa = 10.5SIMTNLKK287 pKa = 9.7LCNICNVDD295 pKa = 3.49EE296 pKa = 4.83FFCTFEE302 pKa = 4.93KK303 pKa = 11.0YY304 pKa = 7.91MFRR307 pKa = 11.84IPIGEE312 pKa = 4.09HH313 pKa = 5.91PEE315 pKa = 3.73WVQMYY320 pKa = 9.61LRR322 pKa = 11.84KK323 pKa = 9.7IPFVGEE329 pKa = 3.38QAYY332 pKa = 9.76NQFMANAPEE341 pKa = 4.02TSKK344 pKa = 10.98PSLAAAHH351 pKa = 6.28RR352 pKa = 11.84VVKK355 pKa = 10.71DD356 pKa = 3.71LLNQKK361 pKa = 10.1CLDD364 pKa = 3.62ALQTKK369 pKa = 9.36KK370 pKa = 10.72LKK372 pKa = 10.67KK373 pKa = 10.36FSTKK377 pKa = 10.02CCPKK381 pKa = 10.62LIPQNLEE388 pKa = 3.6IGCPAPKK395 pKa = 10.04KK396 pKa = 6.64WTNRR400 pKa = 11.84KK401 pKa = 8.73KK402 pKa = 10.19KK403 pKa = 10.23SYY405 pKa = 10.06RR406 pKa = 11.84KK407 pKa = 9.22SSKK410 pKa = 9.16QRR412 pKa = 11.84SMTKK416 pKa = 9.7SYY418 pKa = 10.25RR419 pKa = 11.84GKK421 pKa = 10.25RR422 pKa = 11.84RR423 pKa = 11.84KK424 pKa = 6.84TTYY427 pKa = 9.63QPRR430 pKa = 11.84KK431 pKa = 8.51YY432 pKa = 10.14FRR434 pKa = 11.84KK435 pKa = 10.11KK436 pKa = 9.71SAKK439 pKa = 8.21TDD441 pKa = 3.27KK442 pKa = 10.93KK443 pKa = 10.84KK444 pKa = 10.66NCPKK448 pKa = 10.56GKK450 pKa = 9.91SSCKK454 pKa = 9.7CWICNMEE461 pKa = 3.79GHH463 pKa = 6.28YY464 pKa = 11.37ANDD467 pKa = 3.37CPEE470 pKa = 3.96RR471 pKa = 11.84NKK473 pKa = 10.59NSKK476 pKa = 7.03TVKK479 pKa = 10.15FLQTLDD485 pKa = 3.3QMGYY489 pKa = 10.41EE490 pKa = 4.04PVEE493 pKa = 4.75DD494 pKa = 3.89IFDD497 pKa = 4.13GEE499 pKa = 4.24QEE501 pKa = 4.05LFYY504 pKa = 10.67FDD506 pKa = 3.95EE507 pKa = 4.6VEE509 pKa = 4.18PGEE512 pKa = 4.2TSEE515 pKa = 4.57EE516 pKa = 4.05EE517 pKa = 4.2SSEE520 pKa = 4.54DD521 pKa = 3.52EE522 pKa = 4.14

Molecular weight: 59.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2D1M8|B2D1M8_9VIRU Protein 3 OS=Eupatorium vein clearing virus OX=515444 PE=3 SV=1
MM1 pKa = 7.4ANKK4 pKa = 9.31MLTYY8 pKa = 9.01PHH10 pKa = 7.47IYY12 pKa = 9.9KK13 pKa = 10.47KK14 pKa = 10.97GLIYY18 pKa = 10.2EE19 pKa = 4.74FKK21 pKa = 11.15SLDD24 pKa = 3.65DD25 pKa = 3.63KK26 pKa = 11.78SNPRR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 8.37TFSSDD37 pKa = 3.1EE38 pKa = 4.0NSAGLKK44 pKa = 10.05PIIRR48 pKa = 11.84HH49 pKa = 5.87LNNINQITARR59 pKa = 11.84NWLKK63 pKa = 9.58LTKK66 pKa = 10.35LLAYY70 pKa = 10.58LGLEE74 pKa = 3.83KK75 pKa = 10.9DD76 pKa = 3.54KK77 pKa = 11.65TNGLSKK83 pKa = 10.64KK84 pKa = 9.83PSPWDD89 pKa = 3.24QLLKK93 pKa = 10.6DD94 pKa = 3.51IEE96 pKa = 4.79KK97 pKa = 10.1IFHH100 pKa = 6.82RR101 pKa = 11.84SSSSKK106 pKa = 11.22GNDD109 pKa = 3.07QTKK112 pKa = 10.38LLEE115 pKa = 4.16KK116 pKa = 10.7LEE118 pKa = 4.79EE119 pKa = 4.35ISHH122 pKa = 6.54KK123 pKa = 10.58LPKK126 pKa = 10.32AEE128 pKa = 3.82NLVTRR133 pKa = 11.84EE134 pKa = 4.07EE135 pKa = 3.89LSGIIKK141 pKa = 10.31SFHH144 pKa = 6.58EE145 pKa = 3.94EE146 pKa = 3.77LKK148 pKa = 10.38EE149 pKa = 3.94VKK151 pKa = 10.75SMIKK155 pKa = 10.23SVIGG159 pKa = 3.42

Molecular weight: 18.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2267

126

674

377.8

43.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.896 ± 0.628

1.456 ± 0.36

5.249 ± 0.499

7.322 ± 0.648

4.191 ± 0.418

4.852 ± 0.133

2.338 ± 0.338

7.543 ± 0.982

9.881 ± 0.685

8.91 ± 0.496

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.274

6.22 ± 0.628

5.249 ± 0.808

4.102 ± 0.333

3.705 ± 0.43

7.719 ± 0.475

5.293 ± 0.384

4.808 ± 0.584

0.97 ± 0.219

3.264 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski