Vibrio phage Aphrodite1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Gorgonvirinae; Aphroditevirus; Vibrio virus Aphrodite1

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7QI64|A0A2I7QI64_9CAUD Uncharacterized protein OS=Vibrio phage Aphrodite1 OX=2070057 GN=Aphrodite1_0024 PE=4 SV=1
MM1 pKa = 7.72TDD3 pKa = 3.82FTEE6 pKa = 4.24MTQAEE11 pKa = 4.53LLHH14 pKa = 6.58AFFYY18 pKa = 9.77TAYY21 pKa = 9.33EE22 pKa = 4.07NTVSFGRR29 pKa = 11.84QAAVEE34 pKa = 3.86QAIAGMFTNLLRR46 pKa = 11.84SIVIIEE52 pKa = 4.5KK53 pKa = 10.41FDD55 pKa = 3.88DD56 pKa = 4.54FPWLIIQSEE65 pKa = 4.18ITAICIQMVEE75 pKa = 4.9DD76 pKa = 4.32IDD78 pKa = 6.01DD79 pKa = 3.8ILSGNEE85 pKa = 3.74LIAYY89 pKa = 8.26NPLPNVEE96 pKa = 4.26YY97 pKa = 9.31PKK99 pKa = 11.57AMMLEE104 pKa = 4.33IVHH107 pKa = 6.2EE108 pKa = 4.5CC109 pKa = 3.87

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7QI14|A0A2I7QI14_9CAUD Uncharacterized protein OS=Vibrio phage Aphrodite1 OX=2070057 GN=Aphrodite1_0208 PE=4 SV=1
MM1 pKa = 7.74LIKK4 pKa = 10.54KK5 pKa = 9.72LLYY8 pKa = 9.57PRR10 pKa = 11.84TVDD13 pKa = 3.72GFLDD17 pKa = 3.56QLEE20 pKa = 4.53EE21 pKa = 4.22KK22 pKa = 10.95VMALSSVKK30 pKa = 9.83TNNYY34 pKa = 8.48YY35 pKa = 10.97DD36 pKa = 4.26LVFATLRR43 pKa = 11.84EE44 pKa = 4.28EE45 pKa = 4.43YY46 pKa = 9.79EE47 pKa = 3.53NWMEE51 pKa = 4.34LRR53 pKa = 11.84VFPKK57 pKa = 10.16IKK59 pKa = 9.84EE60 pKa = 3.82QRR62 pKa = 11.84QTVRR66 pKa = 11.84LVNVSIKK73 pKa = 9.46TNNRR77 pKa = 11.84NRR79 pKa = 11.84EE80 pKa = 3.9RR81 pKa = 11.84LFFRR85 pKa = 11.84LPVVVGSRR93 pKa = 11.84MDD95 pKa = 3.23KK96 pKa = 10.86KK97 pKa = 10.47EE98 pKa = 4.02CSKK101 pKa = 11.03KK102 pKa = 9.64NHH104 pKa = 6.16FCRR107 pKa = 11.84GYY109 pKa = 11.12DD110 pKa = 3.51EE111 pKa = 5.16TDD113 pKa = 3.12LDD115 pKa = 4.64LLRR118 pKa = 11.84KK119 pKa = 9.8SSPLLVSYY127 pKa = 10.7HH128 pKa = 6.35DD129 pKa = 3.99TPVHH133 pKa = 5.64IRR135 pKa = 11.84GLTSPHH141 pKa = 5.78NFYY144 pKa = 11.12TNVEE148 pKa = 4.11FSLVRR153 pKa = 11.84RR154 pKa = 11.84NTGLQRR160 pKa = 11.84RR161 pKa = 11.84IDD163 pKa = 4.17RR164 pKa = 11.84LRR166 pKa = 11.84MWGPHH171 pKa = 5.83LPWVRR176 pKa = 11.84NKK178 pKa = 10.49NLLKK182 pKa = 10.86LPVRR186 pKa = 11.84HH187 pKa = 6.22

Molecular weight: 22.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

198

0

198

72999

37

3529

368.7

41.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.559 ± 0.114

0.718 ± 0.049

6.554 ± 0.105

7.489 ± 0.127

4.159 ± 0.067

6.464 ± 0.211

2.19 ± 0.099

5.767 ± 0.097

6.017 ± 0.173

9.237 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.103

5.275 ± 0.1

4.143 ± 0.113

3.526 ± 0.091

4.974 ± 0.158

6.352 ± 0.112

6.725 ± 0.261

7.104 ± 0.219

1.175 ± 0.044

3.929 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski