Roseburia phage Jekyll

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509ENC9|A0A509ENC9_9VIRU Uncharacterized protein OS=Roseburia phage Jekyll OX=2570912 PE=4 SV=1
MM1 pKa = 7.66IKK3 pKa = 10.11KK4 pKa = 10.01QYY6 pKa = 10.5TSIDD10 pKa = 3.01WSDD13 pKa = 3.39YY14 pKa = 10.54DD15 pKa = 4.15SNNVVCMDD23 pKa = 4.07TNNSYY28 pKa = 8.22FTAEE32 pKa = 3.88LPEE35 pKa = 4.53DD36 pKa = 3.72EE37 pKa = 5.86RR38 pKa = 11.84EE39 pKa = 4.21LYY41 pKa = 10.98DD42 pKa = 3.42NAEE45 pKa = 4.23TVGIDD50 pKa = 3.37GTII53 pKa = 3.11

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509EQQ5|A0A509EQQ5_9VIRU Minor capsid protein OS=Roseburia phage Jekyll OX=2570912 PE=4 SV=1
MM1 pKa = 7.63ANRR4 pKa = 11.84RR5 pKa = 11.84IGQAGLALIKK15 pKa = 10.51QFEE18 pKa = 4.45SCRR21 pKa = 11.84LIAYY25 pKa = 7.54QCSAGVWTIGYY36 pKa = 8.21GHH38 pKa = 6.46TVGVYY43 pKa = 9.85KK44 pKa = 10.7GMKK47 pKa = 7.95ITQKK51 pKa = 10.59KK52 pKa = 9.31AEE54 pKa = 4.53AYY56 pKa = 10.32LLQDD60 pKa = 3.03VAKK63 pKa = 9.8FEE65 pKa = 4.72KK66 pKa = 10.74YY67 pKa = 10.03INNPSYY73 pKa = 11.66VPFTAQLNQNQFDD86 pKa = 4.22ALVSFAFNLGQGNVKK101 pKa = 9.97KK102 pKa = 10.55LCTGRR107 pKa = 11.84VMNQIPSAMQRR118 pKa = 11.84YY119 pKa = 8.47CKK121 pKa = 10.17AAGKK125 pKa = 7.27TLPGLQRR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84KK135 pKa = 10.2AEE137 pKa = 3.57AALYY141 pKa = 8.29NKK143 pKa = 9.92KK144 pKa = 10.27VEE146 pKa = 4.35SCTGATTTTVKK157 pKa = 10.51EE158 pKa = 4.25SEE160 pKa = 4.51DD161 pKa = 3.61YY162 pKa = 11.81SMNTIKK168 pKa = 10.62KK169 pKa = 8.06GSKK172 pKa = 9.65GNAVKK177 pKa = 10.31VWQIIIGTTADD188 pKa = 4.24GIFGSGTEE196 pKa = 4.22NMTKK200 pKa = 8.37TWQKK204 pKa = 10.37NHH206 pKa = 5.72GLMADD211 pKa = 4.45GIVGKK216 pKa = 10.18NSWKK220 pKa = 9.59TGLEE224 pKa = 4.15SLL226 pKa = 4.82

Molecular weight: 24.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14044

47

868

203.5

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.487 ± 0.337

1.616 ± 0.161

6.551 ± 0.22

8.224 ± 0.363

3.817 ± 0.185

6.615 ± 0.295

1.716 ± 0.16

7.412 ± 0.33

8.196 ± 0.313

7.014 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.048 ± 0.156

5.461 ± 0.194

2.805 ± 0.228

3.596 ± 0.199

4.137 ± 0.307

6.152 ± 0.412

5.995 ± 0.284

5.654 ± 0.16

1.203 ± 0.11

4.301 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski