Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Aliivibrio; Aliivibrio fischeri

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3813 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5E0B6|Q5E0B6_ALIF1 Transcription-repair-coupling factor OS=Aliivibrio fischeri (strain ATCC 700601 / ES114) OX=312309 GN=mfd PE=3 SV=2
MM1 pKa = 7.91KK2 pKa = 10.1YY3 pKa = 9.99KK4 pKa = 10.49QLIPIIALATASIPIHH20 pKa = 5.49ATIVDD25 pKa = 4.39LDD27 pKa = 3.91FSNHH31 pKa = 6.43IEE33 pKa = 4.07DD34 pKa = 4.12TNLNNSFGPSYY45 pKa = 10.23DD46 pKa = 3.37GPVMHH51 pKa = 7.04FLNVGVHH58 pKa = 5.55NGKK61 pKa = 8.48TIDD64 pKa = 3.53AKK66 pKa = 10.37ISSRR70 pKa = 11.84IIGDD74 pKa = 3.29ATFLYY79 pKa = 7.73HH80 pKa = 5.97TPNYY84 pKa = 10.08KK85 pKa = 10.25EE86 pKa = 5.4GSTQPSGDD94 pKa = 3.16IGFLYY99 pKa = 8.57QTNSPGPAGLIYY111 pKa = 10.45TFEE114 pKa = 4.47FFDD117 pKa = 4.05GTDD120 pKa = 3.54GLSGTFSIPYY130 pKa = 7.37TIPEE134 pKa = 4.21FEE136 pKa = 4.48MIGYY140 pKa = 9.73DD141 pKa = 3.27IDD143 pKa = 4.11GEE145 pKa = 4.46PVQSEE150 pKa = 3.96QVRR153 pKa = 11.84VYY155 pKa = 10.72KK156 pKa = 10.98NEE158 pKa = 3.81GFFSYY163 pKa = 8.81QTGSAGASLTAEE175 pKa = 4.28EE176 pKa = 5.0SPDD179 pKa = 3.47GLSVLFTGPGTNYY192 pKa = 10.61NEE194 pKa = 4.47TDD196 pKa = 2.7TSGAVKK202 pKa = 8.87FTYY205 pKa = 10.46KK206 pKa = 9.25NTSIVTLQFEE216 pKa = 4.8TVTASNSPLPNPIFSAFDD234 pKa = 3.9GNWDD238 pKa = 4.24LDD240 pKa = 4.07DD241 pKa = 4.0FTPPIGSSDD250 pKa = 3.44EE251 pKa = 4.46SDD253 pKa = 4.31FGDD256 pKa = 5.22APDD259 pKa = 4.5SYY261 pKa = 11.35NTLKK265 pKa = 11.3SNDD268 pKa = 3.8GAEE271 pKa = 4.25HH272 pKa = 6.88AMTSTLYY279 pKa = 10.81LGSSVDD285 pKa = 4.06ADD287 pKa = 3.51TDD289 pKa = 3.9GQPGIASNGDD299 pKa = 3.68DD300 pKa = 5.14LDD302 pKa = 4.12VDD304 pKa = 4.56GNDD307 pKa = 4.44DD308 pKa = 4.43DD309 pKa = 6.44GITILTSLEE318 pKa = 4.15KK319 pKa = 11.04GLDD322 pKa = 3.23ALINVNASGSGYY334 pKa = 9.58LQAWADD340 pKa = 2.97WDD342 pKa = 3.99MNGSFDD348 pKa = 4.34EE349 pKa = 5.0GEE351 pKa = 4.31QILTNHH357 pKa = 7.44PIVSGVQIVPIRR369 pKa = 11.84VSDD372 pKa = 3.64SATIGSVQTRR382 pKa = 11.84FRR384 pKa = 11.84LSSNPNIPSSGYY396 pKa = 9.6VGDD399 pKa = 5.05GEE401 pKa = 4.47VEE403 pKa = 4.34DD404 pKa = 4.31YY405 pKa = 11.5VFDD408 pKa = 3.77VTDD411 pKa = 4.27PGTTIQHH418 pKa = 5.47SGYY421 pKa = 8.33YY422 pKa = 8.46TAAFEE427 pKa = 5.51DD428 pKa = 3.88NWPEE432 pKa = 4.65IGDD435 pKa = 3.69FDD437 pKa = 6.1LNDD440 pKa = 3.0VVAYY444 pKa = 9.76YY445 pKa = 8.33RR446 pKa = 11.84TTIVSKK452 pKa = 10.69DD453 pKa = 3.52GEE455 pKa = 4.41VLRR458 pKa = 11.84FDD460 pKa = 3.16ITGTIMAYY468 pKa = 9.6GASYY472 pKa = 11.43GNGLGWKK479 pKa = 9.95LNGFSEE485 pKa = 4.56SDD487 pKa = 3.28INLSLARR494 pKa = 11.84LEE496 pKa = 4.47KK497 pKa = 11.05NGVTRR502 pKa = 11.84ANISPFTGEE511 pKa = 4.6DD512 pKa = 3.16KK513 pKa = 11.16SIASPGGDD521 pKa = 2.85LVVVASLNLRR531 pKa = 11.84EE532 pKa = 4.77DD533 pKa = 3.43IIINEE538 pKa = 3.98EE539 pKa = 4.36CKK541 pKa = 10.22FHH543 pKa = 6.43RR544 pKa = 11.84TNPSCSASLEE554 pKa = 4.23SEE556 pKa = 3.8QMTFSISLPFNDD568 pKa = 4.07GSEE571 pKa = 4.24PSVSSLLPLNGADD584 pKa = 3.74PFIFAAGYY592 pKa = 9.95GLYY595 pKa = 10.44HH596 pKa = 7.12GDD598 pKa = 3.88SFSTAPGTDD607 pKa = 3.9LEE609 pKa = 4.31IHH611 pKa = 6.09TADD614 pKa = 4.54FPPTSRR620 pKa = 11.84GTLVSSFYY628 pKa = 11.12GIAQDD633 pKa = 4.99DD634 pKa = 4.23SDD636 pKa = 4.44PATSKK641 pKa = 10.94YY642 pKa = 10.75YY643 pKa = 9.64RR644 pKa = 11.84TTGNLPWGILISSPWNHH661 pKa = 6.24PSEE664 pKa = 4.69YY665 pKa = 9.65IDD667 pKa = 5.02IGDD670 pKa = 4.87AYY672 pKa = 10.14PDD674 pKa = 3.58FAEE677 pKa = 4.36WATSGGTEE685 pKa = 3.62KK686 pKa = 8.31TTWYY690 pKa = 10.63LNPTASNTWSTADD703 pKa = 3.13

Molecular weight: 75.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1WN16|B1WN16_ALIF1 Uncharacterized protein OS=Aliivibrio fischeri (strain ATCC 700601 / ES114) OX=312309 GN=VF_2608 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84NVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3813

0

3813

1236819

15

3971

324.4

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.975 ± 0.036

1.014 ± 0.015

5.486 ± 0.036

6.572 ± 0.04

4.249 ± 0.026

6.545 ± 0.038

2.164 ± 0.021

7.036 ± 0.035

5.882 ± 0.04

10.201 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.021

4.741 ± 0.032

3.699 ± 0.025

4.254 ± 0.032

4.038 ± 0.034

6.823 ± 0.035

5.573 ± 0.039

6.719 ± 0.029

1.189 ± 0.016

3.145 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski