Rhodospirillales bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; unclassified Rhodospirillales

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7065 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6A7MGS6|A0A6A7MGS6_9PROT DNA polymerase III subunit gamma/tau OS=Rhodospirillales bacterium OX=2026786 GN=dnaX PE=3 SV=1
MM1 pKa = 7.33PQRR4 pKa = 11.84TSRR7 pKa = 11.84SSALTRR13 pKa = 11.84STSSQTISCCDD24 pKa = 3.62LLHH27 pKa = 6.58TEE29 pKa = 4.54TRR31 pKa = 11.84QDD33 pKa = 3.48DD34 pKa = 3.54HH35 pKa = 7.73GKK37 pKa = 10.01ALNVSVSRR45 pKa = 11.84KK46 pKa = 9.15DD47 pKa = 3.11AAMPIIKK54 pKa = 10.1FGTFGNDD61 pKa = 3.21TLVGTADD68 pKa = 3.73SDD70 pKa = 3.52WLYY73 pKa = 11.64GLGGDD78 pKa = 4.89DD79 pKa = 3.77VLSGGGGEE87 pKa = 5.08DD88 pKa = 4.01YY89 pKa = 11.28LSGGDD94 pKa = 4.74DD95 pKa = 3.49NDD97 pKa = 3.36VLYY100 pKa = 11.09GGDD103 pKa = 3.56EE104 pKa = 4.27DD105 pKa = 4.58PRR107 pKa = 11.84HH108 pKa = 6.04GDD110 pKa = 3.57EE111 pKa = 5.64LDD113 pKa = 4.25GGDD116 pKa = 3.99GDD118 pKa = 4.46DD119 pKa = 3.99WLFGGAGGDD128 pKa = 3.7HH129 pKa = 7.01LLGGAGNDD137 pKa = 3.65HH138 pKa = 7.27LYY140 pKa = 11.2GGADD144 pKa = 3.36NDD146 pKa = 4.18YY147 pKa = 11.13LDD149 pKa = 4.69GGAGADD155 pKa = 3.63VMDD158 pKa = 4.87GGEE161 pKa = 3.94GLDD164 pKa = 3.08RR165 pKa = 11.84VEE167 pKa = 5.02YY168 pKa = 9.35IHH170 pKa = 6.91SPAAVTVNLTTGTGSGGDD188 pKa = 3.47AAGDD192 pKa = 3.64TLTDD196 pKa = 3.37IEE198 pKa = 4.99DD199 pKa = 3.87VIGSDD204 pKa = 4.81FADD207 pKa = 3.47TLIGNGSSNRR217 pKa = 11.84LTGGSGDD224 pKa = 4.68DD225 pKa = 3.76YY226 pKa = 11.54LSGSGGDD233 pKa = 4.07DD234 pKa = 3.58FLHH237 pKa = 6.69GGQGADD243 pKa = 3.6HH244 pKa = 6.58LHH246 pKa = 6.54GGSGSDD252 pKa = 3.14TVSYY256 pKa = 9.43TGSAAGVTVNLEE268 pKa = 4.16ANVVSGGDD276 pKa = 3.5AEE278 pKa = 5.38GDD280 pKa = 3.49TLVSIEE286 pKa = 4.28NVVGSTGADD295 pKa = 3.33VITGNAEE302 pKa = 3.86ANILSGSSGDD312 pKa = 3.75DD313 pKa = 3.09RR314 pKa = 11.84LNGGAGDD321 pKa = 3.81DD322 pKa = 4.28TLNGGQGQDD331 pKa = 3.94DD332 pKa = 5.33LIGGDD337 pKa = 3.67GADD340 pKa = 2.77TFVFSEE346 pKa = 3.89HH347 pKa = 6.3WYY349 pKa = 9.42YY350 pKa = 11.25SPPPVSDD357 pKa = 3.61TSVEE361 pKa = 4.01APDD364 pKa = 4.32YY365 pKa = 11.01IWDD368 pKa = 3.94FNHH371 pKa = 6.95AQGDD375 pKa = 4.18QIDD378 pKa = 4.5LADD381 pKa = 4.64LDD383 pKa = 4.45AVSGVPGDD391 pKa = 3.56QAFSFINTADD401 pKa = 3.66FTGSEE406 pKa = 4.23GEE408 pKa = 3.87LRR410 pKa = 11.84YY411 pKa = 9.85EE412 pKa = 4.05QFTGPFGITWTAVSGDD428 pKa = 3.5MDD430 pKa = 4.24GDD432 pKa = 3.89AVADD436 pKa = 3.98FAIVCTDD443 pKa = 4.02SINFVASDD451 pKa = 3.61FLLL454 pKa = 4.64

Molecular weight:
46.13 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6A7LWW9|A0A6A7LWW9_9PROT Glutathione S-transferase family protein OS=Rhodospirillales bacterium OX=2026786 GN=GEV13_00565 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.33VIANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SGRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.09

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7065

0

7065

2124564

29

3930

300.7

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.536 ± 0.039

0.926 ± 0.009

5.513 ± 0.023

5.287 ± 0.026

3.654 ± 0.018

8.748 ± 0.034

2.118 ± 0.014

4.755 ± 0.021

3.386 ± 0.03

10.062 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.015

2.49 ± 0.018

5.534 ± 0.02

3.061 ± 0.017

7.628 ± 0.037

5.11 ± 0.021

5.208 ± 0.024

7.692 ± 0.025

1.541 ± 0.012

2.268 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski