Flavobacteriaceae bacterium R38

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9EVR1|A0A6L9EVR1_9FLAO DUF2911 domain-containing protein OS=Flavobacteriaceae bacterium R38 OX=2697054 GN=GTQ40_01500 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84FISVFIFLIFFCFSNKK18 pKa = 7.88TFAQNIPPTILVEE31 pKa = 4.21NEE33 pKa = 3.72PFYY36 pKa = 11.47CPLSRR41 pKa = 11.84QNIVTAFDD49 pKa = 4.66IIDD52 pKa = 4.18PDD54 pKa = 3.79DD55 pKa = 4.12TEE57 pKa = 4.47VEE59 pKa = 4.03AFFIQISVGYY69 pKa = 10.41DD70 pKa = 2.9EE71 pKa = 5.98GQDD74 pKa = 3.72FLEE77 pKa = 4.43LTNSDD82 pKa = 3.88FHH84 pKa = 8.2PEE86 pKa = 4.01IVAIWNSEE94 pKa = 3.9EE95 pKa = 4.13GRR97 pKa = 11.84LTLEE101 pKa = 4.11GVGGIMIDD109 pKa = 3.45YY110 pKa = 9.78DD111 pKa = 4.39AIIAAVNDD119 pKa = 3.21IVFFNTDD126 pKa = 3.67PDD128 pKa = 3.75NTTSKK133 pKa = 10.56SFSYY137 pKa = 10.53TIGDD141 pKa = 3.93ANFLPSTGHH150 pKa = 6.87FYY152 pKa = 11.09QFIPDD157 pKa = 4.34IGISWIAAEE166 pKa = 4.08QAASNLTFFGLQGYY180 pKa = 8.27LATLTSAEE188 pKa = 4.13EE189 pKa = 3.79AQFAGNQITGNGWIGGSDD207 pKa = 3.25AAEE210 pKa = 3.89EE211 pKa = 4.69GVWRR215 pKa = 11.84WVTGPEE221 pKa = 3.55NGMVFWNGGVNGSTPNFANWNTGEE245 pKa = 4.01PNQLGNEE252 pKa = 4.3DD253 pKa = 3.98YY254 pKa = 11.79AHH256 pKa = 6.26ITSPGIGIDD265 pKa = 4.03GSWNDD270 pKa = 4.2LRR272 pKa = 11.84NEE274 pKa = 4.39GDD276 pKa = 3.18PSGPFQSMGYY286 pKa = 7.97VVEE289 pKa = 4.18YY290 pKa = 10.66GGLPGDD296 pKa = 3.55PVINISASTTFMIPTILSVSNDD318 pKa = 3.04INSCGPVEE326 pKa = 4.39VDD328 pKa = 3.97LTATINGEE336 pKa = 4.13RR337 pKa = 11.84IFWFASEE344 pKa = 4.1TSDD347 pKa = 4.59VILHH351 pKa = 5.97TGIPFTVNVEE361 pKa = 3.91NTTTFWVLASVDD373 pKa = 3.46GCLTGVRR380 pKa = 11.84TPVTVMIDD388 pKa = 3.53GLPSINNASLTQCDD402 pKa = 3.28VDD404 pKa = 4.24GVSDD408 pKa = 4.3GSTVFNLNEE417 pKa = 4.04ARR419 pKa = 11.84TALIGDD425 pKa = 4.0ITNVSIQFFTSLIDD439 pKa = 3.62AQNATNAVNSNVFRR453 pKa = 11.84NTVNPQTLFVRR464 pKa = 11.84ANDD467 pKa = 3.53NVSACFSIAEE477 pKa = 4.24LVLDD481 pKa = 4.42VSTTQVNNATLEE493 pKa = 4.19VCDD496 pKa = 3.94TDD498 pKa = 4.21GNEE501 pKa = 4.68DD502 pKa = 3.74GLHH505 pKa = 6.65LFTLSDD511 pKa = 4.06ADD513 pKa = 4.92DD514 pKa = 5.01EE515 pKa = 4.53ILNGLTNNGDD525 pKa = 3.48FDD527 pKa = 3.76IVYY530 pKa = 10.18YY531 pKa = 11.03ASTEE535 pKa = 4.1DD536 pKa = 5.22ALLEE540 pKa = 4.08QNPLNNDD547 pKa = 3.61YY548 pKa = 11.9ANVTPGNQIIYY559 pKa = 10.87ARR561 pKa = 11.84IEE563 pKa = 4.2DD564 pKa = 3.85NNNCFGISQVNLIVNEE580 pKa = 3.9IPILLNPEE588 pKa = 3.83EE589 pKa = 4.22DD590 pKa = 3.71VVYY593 pKa = 10.43CLNSFPEE600 pKa = 4.64TITLEE605 pKa = 4.13GNIFNNDD612 pKa = 3.28SEE614 pKa = 4.51NFSYY618 pKa = 10.33RR619 pKa = 11.84WSTGEE624 pKa = 3.94TTSSIEE630 pKa = 3.85VNQIGVFTLEE640 pKa = 3.93ITNSSGCSNVRR651 pKa = 11.84TITVLPSNVATIDD664 pKa = 3.76EE665 pKa = 4.88IIVNDD670 pKa = 3.63VTSSNTATINVTGEE684 pKa = 3.66GDD686 pKa = 3.63YY687 pKa = 11.47EE688 pKa = 4.09FALDD692 pKa = 3.71SLSGFFQDD700 pKa = 5.08EE701 pKa = 4.18NTFNNISPGFHH712 pKa = 5.28TVFVRR717 pKa = 11.84DD718 pKa = 3.61KK719 pKa = 9.59NGCGVVSKK727 pKa = 9.99LFSVIGFPRR736 pKa = 11.84FFTPNGDD743 pKa = 3.73SYY745 pKa = 11.97NDD747 pKa = 3.03TWQLSGVGNGFNEE760 pKa = 3.75NSLINIFDD768 pKa = 3.89RR769 pKa = 11.84FGKK772 pKa = 10.64LIIQIDD778 pKa = 3.76ANGRR782 pKa = 11.84GWDD785 pKa = 3.58GTFNGNDD792 pKa = 4.13LPASDD797 pKa = 3.34YY798 pKa = 9.47WFRR801 pKa = 11.84VRR803 pKa = 11.84LEE805 pKa = 4.03DD806 pKa = 3.47GRR808 pKa = 11.84IFSGHH813 pKa = 6.14FSLIRR818 pKa = 4.07

Molecular weight: 89.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9EWJ7|A0A6L9EWJ7_9FLAO Chorismate synthase OS=Flavobacteriaceae bacterium R38 OX=2697054 GN=aroC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.52LSVSSEE47 pKa = 3.87PRR49 pKa = 11.84HH50 pKa = 5.92KK51 pKa = 10.61KK52 pKa = 9.84

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3541

0

3541

1175463

38

4194

332.0

37.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.001 ± 0.037

0.696 ± 0.011

5.603 ± 0.034

6.776 ± 0.043

5.382 ± 0.041

6.281 ± 0.044

1.761 ± 0.019

8.467 ± 0.042

8.022 ± 0.06

9.36 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.021

6.335 ± 0.041

3.317 ± 0.027

3.239 ± 0.019

3.609 ± 0.029

6.472 ± 0.032

5.647 ± 0.055

6.003 ± 0.038

1.041 ± 0.018

4.022 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski