Anaerovibrio sp. JC8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Anaerovibrio; unclassified Anaerovibrio

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1Q4Q1|A0A1X1Q4Q1_9FIRM Methyl-accepting chemotaxis protein OS=Anaerovibrio sp. JC8 OX=1240085 GN=D081_1798 PE=4 SV=1
MM1 pKa = 7.7HH2 pKa = 7.36ILEE5 pKa = 4.57VLKK8 pKa = 11.13KK9 pKa = 9.05MDD11 pKa = 5.13EE12 pKa = 3.98ITTAIIDD19 pKa = 3.97TEE21 pKa = 4.29SEE23 pKa = 4.28EE24 pKa = 4.8KK25 pKa = 10.84NILYY29 pKa = 10.14SDD31 pKa = 3.91QLQDD35 pKa = 4.13IYY37 pKa = 11.58QDD39 pKa = 3.56LQAFYY44 pKa = 9.86PQMVANAAEE53 pKa = 4.26YY54 pKa = 11.08KK55 pKa = 10.56LISDD59 pKa = 4.18FLAYY63 pKa = 10.42DD64 pKa = 5.11LIDD67 pKa = 3.59VDD69 pKa = 5.59EE70 pKa = 5.75IDD72 pKa = 3.02IDD74 pKa = 3.5IYY76 pKa = 10.96MFGGRR81 pKa = 11.84LLEE84 pKa = 5.07LILAFEE90 pKa = 5.1GVQQ93 pKa = 3.19

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1Q4T4|A0A1X1Q4T4_9FIRM 3-isopropylmalate dehydratase small subunit OS=Anaerovibrio sp. JC8 OX=1240085 GN=leuD PE=3 SV=1
MM1 pKa = 8.07SFMSGIMMGAAIGRR15 pKa = 11.84NIHH18 pKa = 6.27EE19 pKa = 4.85IIGGKK24 pKa = 9.5KK25 pKa = 8.1RR26 pKa = 11.84WKK28 pKa = 10.26GGADD32 pKa = 3.34GRR34 pKa = 11.84RR35 pKa = 11.84LNAGRR40 pKa = 11.84CKK42 pKa = 10.52SNQASVEE49 pKa = 3.92NFALVSKK56 pKa = 10.75LPGRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84YY63 pKa = 10.25RR64 pKa = 11.84IASLINNSALSAMLEE79 pKa = 4.19KK80 pKa = 10.55KK81 pKa = 10.45LKK83 pKa = 9.09EE84 pKa = 3.97EE85 pKa = 3.85KK86 pKa = 10.12HH87 pKa = 5.44ISRR90 pKa = 11.84VEE92 pKa = 4.1VNSLTGSVLLIHH104 pKa = 6.95SMDD107 pKa = 3.7EE108 pKa = 4.17GALDD112 pKa = 4.15LLMEE116 pKa = 4.35RR117 pKa = 11.84VKK119 pKa = 11.06NRR121 pKa = 11.84IMPQSSYY128 pKa = 10.85CGCGQGNWGRR138 pKa = 11.84NPVLYY143 pKa = 10.37AANWHH148 pKa = 5.48NTANSINNQVRR159 pKa = 11.84SLTYY163 pKa = 10.25GWFDD167 pKa = 4.28LPTLLSVFFLIRR179 pKa = 11.84GLRR182 pKa = 11.84KK183 pKa = 8.74MLLYY187 pKa = 10.2GQRR190 pKa = 11.84PSGPTMVWWALHH202 pKa = 4.46MMKK205 pKa = 10.15GRR207 pKa = 11.84KK208 pKa = 8.23HH209 pKa = 5.55

Molecular weight: 23.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2405

0

2405

779044

37

2391

323.9

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.281 ± 0.053

1.212 ± 0.019

5.997 ± 0.034

6.959 ± 0.057

3.879 ± 0.039

7.546 ± 0.077

1.835 ± 0.024

7.136 ± 0.043

6.631 ± 0.045

8.733 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.253 ± 0.028

4.659 ± 0.051

3.48 ± 0.033

3.272 ± 0.032

4.331 ± 0.034

5.695 ± 0.042

5.29 ± 0.052

7.243 ± 0.043

0.888 ± 0.017

3.681 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski