Mycobacterium phage KristaRAM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DZX9|A0A385DZX9_9CAUD Uncharacterized protein OS=Mycobacterium phage KristaRAM OX=2301700 GN=48 PE=4 SV=1
MM1 pKa = 7.37SHH3 pKa = 5.68EE4 pKa = 4.77HH5 pKa = 7.34GEE7 pKa = 3.98IDD9 pKa = 5.14PISQLEE15 pKa = 4.11LDD17 pKa = 3.99VLEE20 pKa = 5.24SGDD23 pKa = 4.97LEE25 pKa = 4.79LATQVGEE32 pKa = 4.3VARR35 pKa = 11.84WAYY38 pKa = 8.21ATTVEE43 pKa = 4.98SIWALLCLPSEE54 pKa = 4.6VAEE57 pKa = 3.99QMVNEE62 pKa = 4.22WFLRR66 pKa = 3.83

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DZF2|A0A385DZF2_9CAUD Uncharacterized protein OS=Mycobacterium phage KristaRAM OX=2301700 GN=10 PE=4 SV=1
MM1 pKa = 6.67TTFPAPRR8 pKa = 11.84TLTEE12 pKa = 5.09RR13 pKa = 11.84IQGAHH18 pKa = 6.7LNLKK22 pKa = 9.83LARR25 pKa = 11.84QAGNPDD31 pKa = 3.49IIAAAEE37 pKa = 4.34RR38 pKa = 11.84ILNQLVDD45 pKa = 4.1RR46 pKa = 11.84LPRR49 pKa = 11.84STSQEE54 pKa = 3.91KK55 pKa = 10.38

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

18907

30

1176

167.3

18.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.731 ± 0.382

1.402 ± 0.179

6.543 ± 0.23

5.95 ± 0.361

3.036 ± 0.173

8.938 ± 0.649

2.221 ± 0.184

4.337 ± 0.193

3.242 ± 0.206

7.32 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.142

3.311 ± 0.179

5.977 ± 0.207

3.364 ± 0.204

6.934 ± 0.439

5.86 ± 0.297

6.574 ± 0.263

7.146 ± 0.308

2.322 ± 0.133

2.539 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski