Gallus gallus (Chicken)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q2UD04|A0A3Q2UD04_CHICK Isoform of A0A3Q2UFH9 Uncharacterized protein OS=Gallus gallus OX=9031 GN=EML4 PE=3 SV=1
MM1 pKa = 7.2SVDD4 pKa = 3.8MNSQGSDD11 pKa = 3.52SNEE14 pKa = 3.36EE15 pKa = 4.38DD16 pKa = 3.49YY17 pKa = 11.52DD18 pKa = 4.19PNCEE22 pKa = 4.03EE23 pKa = 4.43EE24 pKa = 5.63EE25 pKa = 4.08EE26 pKa = 5.18DD27 pKa = 4.46EE28 pKa = 5.03DD29 pKa = 5.11PGDD32 pKa = 3.93IEE34 pKa = 5.71DD35 pKa = 4.77YY36 pKa = 10.96YY37 pKa = 11.56DD38 pKa = 3.85GVANDD43 pKa = 4.14VEE45 pKa = 4.56QQGADD50 pKa = 3.01AFDD53 pKa = 4.0PEE55 pKa = 5.06EE56 pKa = 3.99YY57 pKa = 10.58QFTCLTYY64 pKa = 10.33KK65 pKa = 10.6EE66 pKa = 4.64SEE68 pKa = 4.29STLNEE73 pKa = 3.81HH74 pKa = 6.27MASLAATLKK83 pKa = 10.75NLISSSTIFLTSFITLEE100 pKa = 4.32DD101 pKa = 4.04FPLRR105 pKa = 11.84QSSFLLALFLSLLVRR120 pKa = 11.84RR121 pKa = 11.84CFSSAALDD129 pKa = 3.53MSIGHH134 pKa = 5.63VV135 pKa = 3.29

Molecular weight: 15.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q98SR1|Q98SR1_CHICK Claudin OS=Gallus gallus OX=9031 GN=cldn5 PE=2 SV=1
MM1 pKa = 7.45TSRR4 pKa = 11.84IRR6 pKa = 11.84CTVRR10 pKa = 11.84LRR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84SRR17 pKa = 11.84PLSFSLLRR25 pKa = 11.84RR26 pKa = 11.84ILRR29 pKa = 11.84GVAAVLRR36 pKa = 11.84RR37 pKa = 11.84SGTLRR42 pKa = 11.84RR43 pKa = 11.84ILRR46 pKa = 11.84RR47 pKa = 11.84VLRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 5.61RR54 pKa = 11.84GSRR57 pKa = 11.84RR58 pKa = 11.84PRR60 pKa = 3.57

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18113

9678

27791

17623393

5

8377

634.1

70.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.037 ± 0.014

2.254 ± 0.013

4.914 ± 0.01

7.164 ± 0.023

3.514 ± 0.01

6.431 ± 0.027

2.538 ± 0.008

4.53 ± 0.011

5.931 ± 0.019

9.516 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.006

3.842 ± 0.011

5.916 ± 0.023

4.719 ± 0.015

5.592 ± 0.012

8.453 ± 0.017

5.349 ± 0.012

6.148 ± 0.011

1.211 ± 0.006

2.717 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski