Mycobacterium phage PBI1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dclasvirinae; Plotvirus; Mycobacterium virus PBI1

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19ZF6|Q19ZF6_9CAUD Prok-JAB domain-containing protein OS=Mycobacterium phage PBI1 OX=373410 GN=11 PE=4 SV=1
MM1 pKa = 7.28MIVHH5 pKa = 6.5VLPVDD10 pKa = 3.69DD11 pKa = 5.73LIEE14 pKa = 4.44HH15 pKa = 6.14VEE17 pKa = 4.96SPDD20 pKa = 3.9CPCDD24 pKa = 3.58PGLEE28 pKa = 4.43LIEE31 pKa = 4.47RR32 pKa = 11.84TDD34 pKa = 3.27DD35 pKa = 4.01HH36 pKa = 6.68FGQMYY41 pKa = 9.54SHH43 pKa = 6.93NAADD47 pKa = 3.72GRR49 pKa = 11.84NDD51 pKa = 3.3EE52 pKa = 5.07AEE54 pKa = 4.19EE55 pKa = 4.01EE56 pKa = 4.36DD57 pKa = 4.84LFF59 pKa = 6.4

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19ZF5|Q19ZF5_9CAUD Uncharacterized protein OS=Mycobacterium phage PBI1 OX=373410 GN=12 PE=4 SV=1
MM1 pKa = 7.41QSSQQPCLTLRR12 pKa = 11.84KK13 pKa = 8.8MRR15 pKa = 11.84NKK17 pKa = 10.44AYY19 pKa = 10.37AKK21 pKa = 10.56GLATTDD27 pKa = 3.15LDD29 pKa = 5.2RR30 pKa = 11.84RR31 pKa = 11.84LEE33 pKa = 4.79DD34 pKa = 3.32INCSLRR40 pKa = 11.84GLLHH44 pKa = 5.6VTPSSCFSEE53 pKa = 4.44TCIQRR58 pKa = 11.84SQRR61 pKa = 11.84VNRR64 pKa = 11.84RR65 pKa = 11.84QRR67 pKa = 11.84SRR69 pKa = 11.84RR70 pKa = 11.84SKK72 pKa = 10.67QDD74 pKa = 2.74STTIAAFF81 pKa = 3.56

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

20069

41

1590

247.8

27.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.248 ± 0.317

0.967 ± 0.129

6.921 ± 0.32

6.916 ± 0.247

3.209 ± 0.133

8.212 ± 0.316

1.913 ± 0.157

4.893 ± 0.235

5.491 ± 0.3

7.629 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.101

3.956 ± 0.158

5.162 ± 0.22

3.603 ± 0.179

6.557 ± 0.356

5.93 ± 0.201

5.88 ± 0.248

6.243 ± 0.235

1.938 ± 0.131

3.084 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski