Ensifer adhaerens OV14

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Ensifer; Ensifer adhaerens

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8IPW3|W8IPW3_ENSAD HTH gntR-type domain-containing protein OS=Ensifer adhaerens OV14 OX=1416753 GN=OV14_b0279 PE=3 SV=1
MM1 pKa = 7.99LYY3 pKa = 8.87GTEE6 pKa = 4.45ANDD9 pKa = 4.61SIWGDD14 pKa = 3.42GSVDD18 pKa = 3.18VTMAGGKK25 pKa = 10.29GDD27 pKa = 4.93DD28 pKa = 3.25IYY30 pKa = 11.78YY31 pKa = 10.19LYY33 pKa = 11.08SGINRR38 pKa = 11.84AMEE41 pKa = 3.91TAGAGVDD48 pKa = 4.64TINTWMSYY56 pKa = 7.27TLPEE60 pKa = 4.06NFEE63 pKa = 4.34NLTVTGSGRR72 pKa = 11.84HH73 pKa = 5.03AFGNNADD80 pKa = 4.45NIISGASGSQTIDD93 pKa = 2.86GGGGNDD99 pKa = 3.11ILVGGSGADD108 pKa = 2.9IFVVGRR114 pKa = 11.84GGGSDD119 pKa = 4.94LIADD123 pKa = 4.71FDD125 pKa = 4.41SSDD128 pKa = 3.45TVRR131 pKa = 11.84LNGYY135 pKa = 9.79DD136 pKa = 3.36LTSFQQVTSLATQEE150 pKa = 4.24GTDD153 pKa = 3.64LRR155 pKa = 11.84LNLGDD160 pKa = 4.18GEE162 pKa = 4.68SLLFADD168 pKa = 4.16TTVADD173 pKa = 4.65LAPEE177 pKa = 3.99QFQLSLDD184 pKa = 3.74RR185 pKa = 11.84SNLALSFADD194 pKa = 3.97EE195 pKa = 4.33FDD197 pKa = 3.92SLSLHH202 pKa = 7.41DD203 pKa = 4.89GDD205 pKa = 5.12QGVWDD210 pKa = 4.54AKK212 pKa = 10.4FWWAPEE218 pKa = 3.94KK219 pKa = 11.08GGTLASNGEE228 pKa = 4.32LQWYY232 pKa = 9.0INPDD236 pKa = 3.44HH237 pKa = 7.31AEE239 pKa = 3.85TAAINPFSVEE249 pKa = 4.36DD250 pKa = 3.83GVLTIKK256 pKa = 10.96AEE258 pKa = 4.05PTPDD262 pKa = 3.37ALKK265 pKa = 10.78PEE267 pKa = 4.18IDD269 pKa = 4.0GYY271 pKa = 11.3DD272 pKa = 3.61YY273 pKa = 11.21TSGMLTTYY281 pKa = 9.61STFAQTYY288 pKa = 8.16GYY290 pKa = 10.67FEE292 pKa = 4.65IRR294 pKa = 11.84ADD296 pKa = 3.66MPTEE300 pKa = 4.11TGTWPAFWLLPADD313 pKa = 4.39GSWPPEE319 pKa = 3.76LDD321 pKa = 3.52VVEE324 pKa = 4.8MRR326 pKa = 11.84GQQPNIVNVTVHH338 pKa = 6.09SNEE341 pKa = 4.11TGEE344 pKa = 4.3QTSEE348 pKa = 3.77TTAVKK353 pKa = 10.24VASTEE358 pKa = 4.03GFHH361 pKa = 6.57TYY363 pKa = 9.81GVLWEE368 pKa = 4.06EE369 pKa = 5.38DD370 pKa = 4.04EE371 pKa = 4.46IVWYY375 pKa = 10.52YY376 pKa = 11.57DD377 pKa = 3.24DD378 pKa = 4.05VAVARR383 pKa = 11.84ADD385 pKa = 3.96TPADD389 pKa = 3.41MHH391 pKa = 7.28DD392 pKa = 3.64PMYY395 pKa = 10.32MVVNLAVGGAAGEE408 pKa = 4.2PNADD412 pKa = 3.67FGDD415 pKa = 4.16GAEE418 pKa = 4.03MQIDD422 pKa = 4.51YY423 pKa = 10.7IHH425 pKa = 7.39AYY427 pKa = 10.27ALDD430 pKa = 4.16EE431 pKa = 4.37VQSATGEE438 pKa = 4.09AQPMDD443 pKa = 3.68DD444 pKa = 3.68WLSS447 pKa = 3.34

Molecular weight: 48.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8IB21|W8IB21_ENSAD Dipeptide ABC transport system permease protein DppB OS=Ensifer adhaerens OV14 OX=1416753 GN=dppB PE=3 SV=1
MM1 pKa = 7.55LLLHH5 pKa = 6.72KK6 pKa = 10.74SSFLVGALCFLYY18 pKa = 10.67LRR20 pKa = 11.84WQSPRR25 pKa = 11.84NEE27 pKa = 4.17GLGILAAGFLFLALASTLSGYY48 pKa = 10.46GEE50 pKa = 4.31QKK52 pKa = 10.68LLPAILWTLSSFTLGLGGYY71 pKa = 9.48ALFWIGLRR79 pKa = 11.84RR80 pKa = 11.84LSGQRR85 pKa = 11.84RR86 pKa = 11.84HH87 pKa = 5.83RR88 pKa = 11.84ADD90 pKa = 3.16WLALAIPLTGAIAGLITHH108 pKa = 6.32FHH110 pKa = 6.55VINSIRR116 pKa = 11.84ASAFNTGAFLFLAASAFAVVADD138 pKa = 5.18RR139 pKa = 11.84KK140 pKa = 10.42SEE142 pKa = 4.02PLPVRR147 pKa = 11.84TIFAASLAIGATLCLAVVVGLLKK170 pKa = 10.37PDD172 pKa = 3.66ILPITTTYY180 pKa = 11.59AFFLLIISHH189 pKa = 6.44FAIALFALVLVKK201 pKa = 10.33EE202 pKa = 4.22RR203 pKa = 11.84AEE205 pKa = 3.82AALRR209 pKa = 11.84RR210 pKa = 11.84TADD213 pKa = 3.07TDD215 pKa = 3.39MLTGVGNRR223 pKa = 11.84RR224 pKa = 11.84WFLSQLPRR232 pKa = 11.84FGWKK236 pKa = 9.85GDD238 pKa = 3.44ALAVMDD244 pKa = 5.16LDD246 pKa = 3.72FFKK249 pKa = 10.82RR250 pKa = 11.84INDD253 pKa = 3.93RR254 pKa = 11.84YY255 pKa = 9.3GHH257 pKa = 5.26QAGDD261 pKa = 3.37QVLIAFAEE269 pKa = 4.24LVRR272 pKa = 11.84ANLRR276 pKa = 11.84ATDD279 pKa = 3.85CFARR283 pKa = 11.84LGGVRR288 pKa = 11.84ALPSEE293 pKa = 4.21NEE295 pKa = 3.93RR296 pKa = 11.84GAGASDD302 pKa = 3.16RR303 pKa = 11.84RR304 pKa = 11.84AVAACHH310 pKa = 5.26RR311 pKa = 11.84TIAHH315 pKa = 6.79PLRR318 pKa = 11.84RR319 pKa = 11.84PAHH322 pKa = 6.15CRR324 pKa = 11.84QCQHH328 pKa = 5.94RR329 pKa = 11.84VCRR332 pKa = 11.84RR333 pKa = 11.84TGCEE337 pKa = 3.74KK338 pKa = 10.52QLGRR342 pKa = 11.84ALQYY346 pKa = 10.49GRR348 pKa = 11.84CRR350 pKa = 11.84TLSGQGCWPP359 pKa = 3.63

Molecular weight: 39.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5422

0

5422

1788006

34

2833

329.8

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.942 ± 0.041

0.793 ± 0.009

5.665 ± 0.026

5.76 ± 0.028

3.958 ± 0.023

8.418 ± 0.029

1.993 ± 0.018

5.699 ± 0.025

3.61 ± 0.028

10.08 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.015

2.803 ± 0.017

4.797 ± 0.021

3.044 ± 0.018

6.715 ± 0.031

5.617 ± 0.023

5.384 ± 0.021

7.54 ± 0.025

1.25 ± 0.012

2.305 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski