Mycobacterium virus Che12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q1A0D4|Q1A0D4_9CAUD Uncharacterized protein OS=Mycobacterium virus Che12 OX=373405 GN=83 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.2EE3 pKa = 4.45VKK5 pKa = 10.59EE6 pKa = 3.86ALNNFLEE13 pKa = 4.47EE14 pKa = 3.85QHH16 pKa = 6.58KK17 pKa = 9.62KK18 pKa = 8.83EE19 pKa = 4.23RR20 pKa = 11.84YY21 pKa = 7.66IEE23 pKa = 4.25SVGGDD28 pKa = 3.71EE29 pKa = 5.88DD30 pKa = 4.51EE31 pKa = 4.98PSCGYY36 pKa = 11.09ADD38 pKa = 4.1YY39 pKa = 11.45DD40 pKa = 4.6EE41 pKa = 6.37AIADD45 pKa = 4.08YY46 pKa = 11.06NEE48 pKa = 3.91SLADD52 pKa = 4.22LGAALADD59 pKa = 4.84AIHH62 pKa = 5.99NQLYY66 pKa = 10.7DD67 pKa = 3.51KK68 pKa = 10.85EE69 pKa = 4.27

Molecular weight:
7.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q1A0G2|Q1A0G2_9CAUD Uncharacterized protein OS=Mycobacterium virus Che12 OX=373405 GN=55 PE=4 SV=1
MM1 pKa = 7.66TSTKK5 pKa = 10.47LPYY8 pKa = 9.42LHH10 pKa = 6.99KK11 pKa = 10.35NARR14 pKa = 11.84SRR16 pKa = 11.84QITSSEE22 pKa = 3.75VRR24 pKa = 11.84EE25 pKa = 4.23VFAEE29 pKa = 4.02EE30 pKa = 4.19VTRR33 pKa = 11.84HH34 pKa = 5.61LDD36 pKa = 3.16RR37 pKa = 11.84RR38 pKa = 11.84LDD40 pKa = 3.57RR41 pKa = 11.84KK42 pKa = 10.73DD43 pKa = 3.18YY44 pKa = 10.61LRR46 pKa = 11.84RR47 pKa = 11.84VMPP50 pKa = 4.81

Molecular weight:
6.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

16156

28

841

164.9

18.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.885 ± 0.391

0.904 ± 0.126

6.282 ± 0.223

6.759 ± 0.267

3.25 ± 0.151

8.09 ± 0.395

2.135 ± 0.169

5.15 ± 0.149

4.809 ± 0.229

8.053 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.135

3.466 ± 0.155

5.416 ± 0.284

3.658 ± 0.197

6.258 ± 0.366

5.342 ± 0.209

5.942 ± 0.225

7.18 ± 0.209

1.931 ± 0.141

3.021 ± 0.19

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski