Planctomycetaceae bacterium SCGC AG-212-F19

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; unclassified Planctomycetaceae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QTI2|A0A177QTI2_9PLAN DUF2294 domain-containing protein OS=Planctomycetaceae bacterium SCGC AG-212-F19 OX=1799658 GN=AYO44_05870 PE=4 SV=1
MM1 pKa = 7.47SANFSWWRR9 pKa = 11.84AGRR12 pKa = 11.84RR13 pKa = 11.84LWNSSVGDD21 pKa = 3.55WLQTGFGAARR31 pKa = 11.84SAPVRR36 pKa = 11.84RR37 pKa = 11.84GKK39 pKa = 10.41GRR41 pKa = 11.84RR42 pKa = 11.84SFRR45 pKa = 11.84PCLEE49 pKa = 3.89MLEE52 pKa = 4.94DD53 pKa = 3.72RR54 pKa = 11.84ALPSASWRR62 pKa = 11.84DD63 pKa = 3.67YY64 pKa = 11.68AHH66 pKa = 7.58DD67 pKa = 4.26PQHH70 pKa = 6.33TGVADD75 pKa = 3.7VASQPLEE82 pKa = 3.94QIHH85 pKa = 5.88WQTPVDD91 pKa = 4.67LNPQFSGSDD100 pKa = 3.51LLIHH104 pKa = 6.29YY105 pKa = 7.67GSPLVTANNTVIVPVKK121 pKa = 9.75TGAADD126 pKa = 3.46GFEE129 pKa = 4.09IEE131 pKa = 5.38GINAATGALKK141 pKa = 8.77WTVTSDD147 pKa = 4.35YY148 pKa = 10.82ILPPHH153 pKa = 6.56NWTPSYY159 pKa = 11.15SPALAPDD166 pKa = 3.19GRR168 pKa = 11.84LYY170 pKa = 10.73FAGVGGTIYY179 pKa = 11.09YY180 pKa = 9.83IDD182 pKa = 4.21NPDD185 pKa = 3.43ANGATVSGHH194 pKa = 4.79IAFYY198 pKa = 10.87GIGNYY203 pKa = 9.69NSSLDD208 pKa = 3.31GTVFINTPLTVDD220 pKa = 3.3SAGNLYY226 pKa = 10.65FGFEE230 pKa = 4.25VTGGNSLGLQSGIARR245 pKa = 11.84IGADD249 pKa = 3.8GTGTWIAASTAAGDD263 pKa = 3.86GNIVKK268 pKa = 9.67VVHH271 pKa = 6.24NCAPALSNDD280 pKa = 3.08GSSLYY285 pKa = 10.66VAVSTGSFGAGYY297 pKa = 10.02LVEE300 pKa = 5.2LNSTTLAPMARR311 pKa = 11.84VLLRR315 pKa = 11.84EE316 pKa = 4.03PLAGNAALLPDD327 pKa = 5.42DD328 pKa = 5.49GSASPTVGPDD338 pKa = 2.68GDD340 pKa = 4.06VYY342 pKa = 11.08FGVLEE347 pKa = 4.13NPFNSSKK354 pKa = 10.64GWLLHH359 pKa = 6.03FSGDD363 pKa = 3.36LSQTKK368 pKa = 8.96TPGAFGWDD376 pKa = 3.4DD377 pKa = 3.45TASIVAASLVPSYY390 pKa = 10.22HH391 pKa = 6.51GSSAYY396 pKa = 11.31LLMTKK401 pKa = 10.09YY402 pKa = 10.98NNYY405 pKa = 10.45AGLGGDD411 pKa = 5.21GINKK415 pKa = 9.41IAILDD420 pKa = 3.92PNATQTDD427 pKa = 4.13PRR429 pKa = 11.84TGATVMQEE437 pKa = 4.29VITIAGQTPDD447 pKa = 4.19PDD449 pKa = 4.02FPGNPGAVRR458 pKa = 11.84EE459 pKa = 4.28WCINTAAVDD468 pKa = 3.96PFTDD472 pKa = 3.4SVVANSEE479 pKa = 4.02DD480 pKa = 3.29GKK482 pKa = 10.93LYY484 pKa = 10.59RR485 pKa = 11.84WDD487 pKa = 3.61LATNTFSQVITLTTATGEE505 pKa = 4.32AYY507 pKa = 9.15TPTLIGPDD515 pKa = 3.37GTVYY519 pKa = 10.87AINRR523 pKa = 11.84ATLFAVGRR531 pKa = 11.84APSGPVTITGTGLFHH546 pKa = 7.56APAIYY551 pKa = 9.06PAGNTPDD558 pKa = 5.14SIAQGDD564 pKa = 3.98FNGDD568 pKa = 3.51GKK570 pKa = 10.22PDD572 pKa = 3.45LVVTNFFGGNVSVLINQGNGTFAAPVHH599 pKa = 5.96YY600 pKa = 8.75ATGSGPNGFVVADD613 pKa = 4.74FNHH616 pKa = 7.07DD617 pKa = 3.34GKK619 pKa = 11.28ADD621 pKa = 3.45IAVGNYY627 pKa = 8.84YY628 pKa = 11.01DD629 pKa = 3.78NAVSVLLGNGDD640 pKa = 3.71GTFQAAVNYY649 pKa = 10.29AVGSGPANEE658 pKa = 4.25MAFGDD663 pKa = 4.15FNGDD667 pKa = 3.24GNLDD671 pKa = 3.65LATANQNDD679 pKa = 4.1GTVSVLLGNGNGTFQPAVTYY699 pKa = 10.4AAGPGSDD706 pKa = 4.29GIVAADD712 pKa = 3.81LNGDD716 pKa = 3.83GKK718 pKa = 11.32LDD720 pKa = 3.8LAVANYY726 pKa = 9.85SGNNISVLLNQGNGTFAAPVTYY748 pKa = 10.57NVGANPWYY756 pKa = 9.95IEE758 pKa = 4.21KK759 pKa = 10.94GDD761 pKa = 3.92FNHH764 pKa = 7.56DD765 pKa = 3.38GTIDD769 pKa = 5.69LITADD774 pKa = 3.9SGGGTVSVLRR784 pKa = 11.84GNGDD788 pKa = 3.25GSFAAAVQYY797 pKa = 9.75TVGGFPDD804 pKa = 5.7GIAVADD810 pKa = 4.2LNGDD814 pKa = 3.77GNLDD818 pKa = 3.82LAVSNYY824 pKa = 7.25STNDD828 pKa = 2.98ASVLFGNADD837 pKa = 3.44GTFQAARR844 pKa = 11.84SVDD847 pKa = 3.34AGAGSGPNGITAADD861 pKa = 3.8FNGDD865 pKa = 3.4GKK867 pKa = 10.61IDD869 pKa = 3.66IASADD874 pKa = 3.75QNAGASVMLSGIVSAVEE891 pKa = 4.12GQSFTGVVASFTTSEE906 pKa = 4.42SNPSASEE913 pKa = 3.83YY914 pKa = 10.19SASINWGDD922 pKa = 3.75GNTSAGTIAADD933 pKa = 3.69GQGGFNVAGTHH944 pKa = 6.16TYY946 pKa = 10.92AEE948 pKa = 4.38EE949 pKa = 4.48GSYY952 pKa = 10.92SLSIVINGPGGASATVANVAGVADD976 pKa = 3.93PAVVVSGTSFTATEE990 pKa = 4.11GQAFSNQLVATFTDD1004 pKa = 3.54PGGAEE1009 pKa = 4.03SFAVSLGDD1017 pKa = 4.56PGFEE1021 pKa = 4.21TPNVGTGFFGAFQYY1035 pKa = 11.17DD1036 pKa = 3.64PTGAPWAYY1044 pKa = 10.49AGGAGVAGNQSGFTSGNPNAPQGTQVGFLQGPGGTISQTFTTGGGNYY1091 pKa = 9.71AVSFQAAQRR1100 pKa = 11.84GNYY1103 pKa = 8.42QPAGNQTIDD1112 pKa = 3.27VLIDD1116 pKa = 3.56GNVVGTFTPWGTNYY1130 pKa = 10.15QSFTTAPIALSGGTHH1145 pKa = 6.03TLTFEE1150 pKa = 4.46GLNSGDD1156 pKa = 3.11TTAFIDD1162 pKa = 3.84TVSIGGSGGGGPYY1175 pKa = 8.9TAAINWGDD1183 pKa = 3.64GSTSAADD1190 pKa = 3.64SIQYY1194 pKa = 10.22NATTGIFSVYY1204 pKa = 10.19GGHH1207 pKa = 6.72TYY1209 pKa = 11.21ASAAGSPLPVSVTIRR1224 pKa = 11.84HH1225 pKa = 5.72EE1226 pKa = 4.04NAPAATATSTATIQDD1241 pKa = 3.73VPLTPIDD1248 pKa = 4.1GPFTSPALYY1257 pKa = 10.33GAGSMPDD1264 pKa = 3.49SIATIRR1270 pKa = 11.84LDD1272 pKa = 3.46NGQVALLVANFGSANVTVLLPNGDD1296 pKa = 3.92GTFHH1300 pKa = 7.07SGGNLAVSNGPNGFAVGDD1318 pKa = 4.14FNHH1321 pKa = 7.36DD1322 pKa = 3.07GHH1324 pKa = 9.1LDD1326 pKa = 3.71FAVGNYY1332 pKa = 9.5YY1333 pKa = 10.89DD1334 pKa = 3.81STVTVYY1340 pKa = 10.81LGNGDD1345 pKa = 3.57GSFRR1349 pKa = 11.84SPAAYY1354 pKa = 9.75AVGANPANTMAVGDD1368 pKa = 4.05FNGDD1372 pKa = 3.29GNLDD1376 pKa = 3.69LATANQGNGTVTVLLGNSDD1395 pKa = 3.84GTFGSAVSYY1404 pKa = 11.07AGGSGNDD1411 pKa = 3.2SVVAVDD1417 pKa = 3.6VNGDD1421 pKa = 3.62GKK1423 pKa = 11.29LDD1425 pKa = 3.86LATANYY1431 pKa = 10.39NDD1433 pKa = 3.67GTASVLINQGNGTFAAPAIISVGSNPWYY1461 pKa = 10.03IDD1463 pKa = 3.2AGDD1466 pKa = 3.75FNKK1469 pKa = 10.42DD1470 pKa = 2.62GRR1472 pKa = 11.84MDD1474 pKa = 4.04LVVANLTGNSVSVLLNQGNGTFSRR1498 pKa = 11.84TDD1500 pKa = 3.31YY1501 pKa = 11.47GVGSWPTAVVVADD1514 pKa = 4.06MNRR1517 pKa = 11.84DD1518 pKa = 3.47GNPDD1522 pKa = 3.74LVVGNYY1528 pKa = 10.13GSTTVSVLLGNGDD1541 pKa = 3.76GTFQAALNFAAGGSGPNGVAVGDD1564 pKa = 3.92FNGDD1568 pKa = 3.44GKK1570 pKa = 10.21PDD1572 pKa = 3.48VAVADD1577 pKa = 3.94QDD1579 pKa = 3.94SGSASVLLNANLVSAVRR1596 pKa = 11.84NEE1598 pKa = 4.24SFTGAVGSFTDD1609 pKa = 4.6ANPLAPPTDD1618 pKa = 4.31FTATIHH1624 pKa = 6.22WGDD1627 pKa = 3.6GTTSTISASAFVRR1640 pKa = 11.84NAAGAFDD1647 pKa = 4.47VIGTHH1652 pKa = 7.02TYY1654 pKa = 8.35TQMGLFDD1661 pKa = 3.86ITIVIQDD1668 pKa = 3.6VGGATTTVSNLVSVHH1683 pKa = 5.63

Molecular weight: 170.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177QJR0|A0A177QJR0_9PLAN Methionine--tRNA ligase OS=Planctomycetaceae bacterium SCGC AG-212-F19 OX=1799658 GN=AYO44_08945 PE=3 SV=1
MM1 pKa = 7.82PAPPPVRR8 pKa = 11.84QAASDD13 pKa = 3.91RR14 pKa = 11.84SALACLRR21 pKa = 11.84MILASHH27 pKa = 6.33GRR29 pKa = 11.84SVTDD33 pKa = 4.27AVLQAPAPIAPGGIPVSDD51 pKa = 4.08LALLAGQFGLEE62 pKa = 4.0AEE64 pKa = 4.14IGTFNLAALTEE75 pKa = 4.1LLEE78 pKa = 4.74AGTYY82 pKa = 9.49PIVYY86 pKa = 9.9LNRR89 pKa = 11.84VHH91 pKa = 7.74LDD93 pKa = 3.04DD94 pKa = 3.48RR95 pKa = 11.84TIPRR99 pKa = 11.84KK100 pKa = 9.26LASRR104 pKa = 11.84RR105 pKa = 11.84CVVHH109 pKa = 6.52AVVPTRR115 pKa = 11.84LSSGFVWLNDD125 pKa = 3.4PRR127 pKa = 11.84SGTLRR132 pKa = 11.84KK133 pKa = 9.18VARR136 pKa = 11.84KK137 pKa = 9.58KK138 pKa = 10.73FEE140 pKa = 4.09AGRR143 pKa = 11.84RR144 pKa = 11.84DD145 pKa = 3.46LSNWCIVCRR154 pKa = 11.84RR155 pKa = 11.84AGRR158 pKa = 11.84GG159 pKa = 3.19

Molecular weight: 17.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3394

0

3394

1134279

58

2974

334.2

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.145 ± 0.056

1.142 ± 0.015

5.592 ± 0.031

5.566 ± 0.039

3.592 ± 0.024

8.218 ± 0.046

2.31 ± 0.019

4.195 ± 0.029

4.299 ± 0.046

10.461 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.02

2.737 ± 0.032

6.16 ± 0.039

3.863 ± 0.028

7.068 ± 0.039

4.727 ± 0.032

5.386 ± 0.04

7.453 ± 0.034

1.673 ± 0.016

2.404 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski