Gordonia phage SallySpecial

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CCU8|A0A2P1CCU8_9CAUD Integrase OS=Gordonia phage SallySpecial OX=2079570 GN=SEA_SALLYSPECIAL_17 PE=3 SV=1
MM1 pKa = 6.75ITAALVVAICALVAALVALVVAGMAVAGVTALDD34 pKa = 3.9CALGRR39 pKa = 11.84DD40 pKa = 3.98TPDD43 pKa = 3.97DD44 pKa = 4.28DD45 pKa = 4.86EE46 pKa = 4.92DD47 pKa = 4.72RR48 pKa = 11.84KK49 pKa = 11.0GLPTT53 pKa = 3.99

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CC00|A0A2P1CC00_9CAUD Tape measure protein OS=Gordonia phage SallySpecial OX=2079570 GN=SEA_SALLYSPECIAL_14 PE=4 SV=1
MM1 pKa = 7.71AGAQVRR7 pKa = 11.84VEE9 pKa = 4.14GARR12 pKa = 11.84EE13 pKa = 3.78LRR15 pKa = 11.84RR16 pKa = 11.84SMRR19 pKa = 11.84RR20 pKa = 11.84AEE22 pKa = 4.33LDD24 pKa = 3.34LAEE27 pKa = 4.94LKK29 pKa = 7.95EE30 pKa = 4.08THH32 pKa = 6.43ARR34 pKa = 11.84VAALVATRR42 pKa = 11.84GQSAAPVQSGRR53 pKa = 11.84LAATVRR59 pKa = 11.84AGATRR64 pKa = 11.84TAAVVRR70 pKa = 11.84AGRR73 pKa = 11.84KK74 pKa = 7.75SVPYY78 pKa = 9.88AGPIHH83 pKa = 6.58WGWPARR89 pKa = 11.84GITAQPWLTTAAAEE103 pKa = 5.08SEE105 pKa = 4.41PAWLAVYY112 pKa = 9.46TEE114 pKa = 4.2AVEE117 pKa = 5.59DD118 pKa = 3.43ILDD121 pKa = 4.41RR122 pKa = 11.84IEE124 pKa = 4.02GARR127 pKa = 11.84GISS130 pKa = 3.22

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

4852

53

635

231.0

24.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.467 ± 1.012

0.701 ± 0.179

5.977 ± 0.536

4.678 ± 0.389

2.432 ± 0.213

9.481 ± 1.141

1.876 ± 0.335

4.101 ± 0.469

2.143 ± 0.287

7.749 ± 0.453

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.731 ± 0.156

2.267 ± 0.313

6.822 ± 0.587

3.03 ± 0.417

7.09 ± 0.627

4.349 ± 0.439

8.017 ± 0.603

7.296 ± 0.478

2.061 ± 0.238

1.731 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski