Zostera marina amalgavirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 8.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W6R5F1|A0A1W6R5F1_9VIRU Fusion protein OS=Zostera marina amalgavirus 2 OX=1985203 PE=4 SV=1
MM1 pKa = 7.64FFRR4 pKa = 11.84CEE6 pKa = 3.51KK7 pKa = 9.48MANEE11 pKa = 5.15DD12 pKa = 3.74GSSVPLKK19 pKa = 9.6TQAQVDD25 pKa = 4.13EE26 pKa = 4.66EE27 pKa = 4.8RR28 pKa = 11.84DD29 pKa = 3.57VAQLNKK35 pKa = 10.25ALSYY39 pKa = 11.09LDD41 pKa = 3.94LGGMPIAPWALQDD54 pKa = 4.62VADD57 pKa = 4.36CSYY60 pKa = 10.67TVARR64 pKa = 11.84ALRR67 pKa = 11.84KK68 pKa = 9.82LKK70 pKa = 10.11ILKK73 pKa = 8.86PHH75 pKa = 6.9YY76 pKa = 10.01DD77 pKa = 3.41NKK79 pKa = 10.5HH80 pKa = 4.56LQKK83 pKa = 10.62LFHH86 pKa = 6.25YY87 pKa = 9.78CEE89 pKa = 4.11EE90 pKa = 4.12NAVIDD95 pKa = 3.65STLPLKK101 pKa = 10.67LSSVFRR107 pKa = 11.84FCDD110 pKa = 3.14WLLSPVAKK118 pKa = 10.23RR119 pKa = 11.84KK120 pKa = 9.61IEE122 pKa = 3.86QLVNADD128 pKa = 3.92RR129 pKa = 11.84LKK131 pKa = 10.82KK132 pKa = 10.14RR133 pKa = 11.84SRR135 pKa = 11.84DD136 pKa = 3.51AITPAEE142 pKa = 4.09TALVAILEE150 pKa = 4.43VAQNDD155 pKa = 3.87CVADD159 pKa = 3.58VSRR162 pKa = 11.84VRR164 pKa = 11.84ITYY167 pKa = 10.24DD168 pKa = 3.34EE169 pKa = 4.69EE170 pKa = 3.99IKK172 pKa = 10.67KK173 pKa = 10.48LKK175 pKa = 10.47RR176 pKa = 11.84KK177 pKa = 9.17IGKK180 pKa = 9.57LEE182 pKa = 3.57EE183 pKa = 3.94HH184 pKa = 7.18KK185 pKa = 10.9ARR187 pKa = 11.84KK188 pKa = 8.86IEE190 pKa = 4.05KK191 pKa = 9.74ARR193 pKa = 11.84KK194 pKa = 8.97KK195 pKa = 10.92YY196 pKa = 10.14PGLLLLEE203 pKa = 4.89RR204 pKa = 11.84PSEE207 pKa = 4.36SDD209 pKa = 3.21VCNQAWHH216 pKa = 6.91KK217 pKa = 10.12YY218 pKa = 8.54VEE220 pKa = 4.3YY221 pKa = 10.76CNASGIKK228 pKa = 8.37QEE230 pKa = 4.09KK231 pKa = 9.62RR232 pKa = 11.84SNASLEE238 pKa = 4.17KK239 pKa = 10.23AISMFDD245 pKa = 3.18NILRR249 pKa = 11.84LEE251 pKa = 4.31IKK253 pKa = 10.19AKK255 pKa = 10.19CCEE258 pKa = 4.0KK259 pKa = 11.0PEE261 pKa = 3.95VRR263 pKa = 11.84DD264 pKa = 4.32YY265 pKa = 11.63LLQYY269 pKa = 10.37CKK271 pKa = 10.75EE272 pKa = 4.15KK273 pKa = 10.79IKK275 pKa = 10.76GFRR278 pKa = 11.84DD279 pKa = 3.39DD280 pKa = 4.07ADD282 pKa = 3.62KK283 pKa = 11.44KK284 pKa = 10.97RR285 pKa = 11.84VDD287 pKa = 3.59TFKK290 pKa = 10.9GYY292 pKa = 10.75YY293 pKa = 9.17SAATGEE299 pKa = 4.25PLPEE303 pKa = 4.51GSSRR307 pKa = 11.84KK308 pKa = 9.42KK309 pKa = 10.39RR310 pKa = 11.84RR311 pKa = 11.84TAASDD316 pKa = 3.3SSGDD320 pKa = 3.9SEE322 pKa = 5.41SDD324 pKa = 3.43GASEE328 pKa = 4.73RR329 pKa = 11.84DD330 pKa = 3.63DD331 pKa = 4.2VVAGDD336 pKa = 3.6FVDD339 pKa = 6.21RR340 pKa = 11.84IFKK343 pKa = 9.75EE344 pKa = 3.99QTSGGEE350 pKa = 4.06QSEE353 pKa = 4.63TPPPQEE359 pKa = 4.24TRR361 pKa = 11.84DD362 pKa = 3.34ADD364 pKa = 3.5IADD367 pKa = 4.02EE368 pKa = 4.25SSHH371 pKa = 6.04GKK373 pKa = 10.05SKK375 pKa = 10.75GKK377 pKa = 9.63SASRR381 pKa = 11.84LRR383 pKa = 11.84SRR385 pKa = 11.84IRR387 pKa = 11.84TRR389 pKa = 11.84AQNRR393 pKa = 11.84KK394 pKa = 9.01KK395 pKa = 10.81

Molecular weight:
44.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W6R5F1|A0A1W6R5F1_9VIRU Fusion protein OS=Zostera marina amalgavirus 2 OX=1985203 PE=4 SV=1
MM1 pKa = 7.64FFRR4 pKa = 11.84CEE6 pKa = 3.51KK7 pKa = 9.48MANEE11 pKa = 5.15DD12 pKa = 3.74GSSVPLKK19 pKa = 9.6TQAQVDD25 pKa = 4.13EE26 pKa = 4.66EE27 pKa = 4.8RR28 pKa = 11.84DD29 pKa = 3.57VAQLNKK35 pKa = 10.25ALSYY39 pKa = 11.09LDD41 pKa = 3.94LGGMPIAPWALQDD54 pKa = 4.62VADD57 pKa = 4.36CSYY60 pKa = 10.67TVARR64 pKa = 11.84ALRR67 pKa = 11.84KK68 pKa = 9.82LKK70 pKa = 10.11ILKK73 pKa = 8.86PHH75 pKa = 6.9YY76 pKa = 10.01DD77 pKa = 3.41NKK79 pKa = 10.5HH80 pKa = 4.56LQKK83 pKa = 10.62LFHH86 pKa = 6.25YY87 pKa = 9.78CEE89 pKa = 4.11EE90 pKa = 4.12NAVIDD95 pKa = 3.65STLPLKK101 pKa = 10.67LSSVFRR107 pKa = 11.84FCDD110 pKa = 3.14WLLSPVAKK118 pKa = 10.23RR119 pKa = 11.84KK120 pKa = 9.61IEE122 pKa = 3.86QLVNADD128 pKa = 3.92RR129 pKa = 11.84LKK131 pKa = 10.82KK132 pKa = 10.14RR133 pKa = 11.84SRR135 pKa = 11.84DD136 pKa = 3.51AITPAEE142 pKa = 4.09TALVAILEE150 pKa = 4.43VAQNDD155 pKa = 3.87CVADD159 pKa = 3.58VSRR162 pKa = 11.84VRR164 pKa = 11.84ITYY167 pKa = 10.24DD168 pKa = 3.34EE169 pKa = 4.69EE170 pKa = 3.99IKK172 pKa = 10.67KK173 pKa = 10.48LKK175 pKa = 10.47RR176 pKa = 11.84KK177 pKa = 9.17IGKK180 pKa = 9.57LEE182 pKa = 3.57EE183 pKa = 3.94HH184 pKa = 7.18KK185 pKa = 10.9ARR187 pKa = 11.84KK188 pKa = 8.86IEE190 pKa = 4.05KK191 pKa = 9.74ARR193 pKa = 11.84KK194 pKa = 8.97KK195 pKa = 10.92YY196 pKa = 10.14PGLLLLEE203 pKa = 4.89RR204 pKa = 11.84PSEE207 pKa = 4.36SDD209 pKa = 3.21VCNQAWHH216 pKa = 6.91KK217 pKa = 10.12YY218 pKa = 8.54VEE220 pKa = 4.3YY221 pKa = 10.76CNASGIKK228 pKa = 8.37QEE230 pKa = 4.09KK231 pKa = 9.62RR232 pKa = 11.84SNASLEE238 pKa = 4.17KK239 pKa = 10.23AISMFDD245 pKa = 3.18NILRR249 pKa = 11.84LEE251 pKa = 4.31IKK253 pKa = 10.19AKK255 pKa = 10.19CCEE258 pKa = 4.0KK259 pKa = 11.0PEE261 pKa = 3.95VRR263 pKa = 11.84DD264 pKa = 4.32YY265 pKa = 11.63LLQYY269 pKa = 10.37CKK271 pKa = 10.67EE272 pKa = 4.13KK273 pKa = 10.72IKK275 pKa = 11.08GFVTTPIKK283 pKa = 10.64SALTHH288 pKa = 6.12SRR290 pKa = 11.84DD291 pKa = 3.59TILQRR296 pKa = 11.84LEE298 pKa = 3.95SHH300 pKa = 6.56FLKK303 pKa = 10.88APLEE307 pKa = 4.1RR308 pKa = 11.84RR309 pKa = 11.84EE310 pKa = 4.16EE311 pKa = 4.24LLPLIPVGTVNPMGHH326 pKa = 7.16LNATMSLRR334 pKa = 11.84EE335 pKa = 4.02ILWTEE340 pKa = 3.83SLKK343 pKa = 10.61NRR345 pKa = 11.84QVEE348 pKa = 4.32GSKK351 pKa = 10.53VKK353 pKa = 10.51PPLPRR358 pKa = 11.84KK359 pKa = 10.15LEE361 pKa = 3.92MLILPMNLLMANPRR375 pKa = 11.84VNLLHH380 pKa = 6.83AFVHH384 pKa = 6.29ASGRR388 pKa = 11.84GLKK391 pKa = 9.5IARR394 pKa = 11.84SKK396 pKa = 10.29WEE398 pKa = 3.87AGVRR402 pKa = 11.84RR403 pKa = 11.84IVGGGEE409 pKa = 3.54MRR411 pKa = 11.84GWNEE415 pKa = 3.6DD416 pKa = 3.24SAKK419 pKa = 10.31YY420 pKa = 10.1RR421 pKa = 11.84GGGNLHH427 pKa = 6.68DD428 pKa = 5.95AIRR431 pKa = 11.84VLATGRR437 pKa = 11.84HH438 pKa = 5.85DD439 pKa = 3.98LPGSFLFQHH448 pKa = 6.31FNFRR452 pKa = 11.84SARR455 pKa = 11.84SILKK459 pKa = 10.23LPSDD463 pKa = 4.24LSVPDD468 pKa = 3.85GRR470 pKa = 11.84DD471 pKa = 3.05SVTMRR476 pKa = 11.84NFNEE480 pKa = 4.09EE481 pKa = 3.71ATAGPVLRR489 pKa = 11.84AIGCKK494 pKa = 8.6TKK496 pKa = 10.83YY497 pKa = 9.63GLKK500 pKa = 10.22RR501 pKa = 11.84GLEE504 pKa = 4.27DD505 pKa = 4.48IVWNLYY511 pKa = 10.66DD512 pKa = 3.38RR513 pKa = 11.84VGNGEE518 pKa = 4.08LRR520 pKa = 11.84TWEE523 pKa = 4.65LPPLLARR530 pKa = 11.84IGFRR534 pKa = 11.84SKK536 pKa = 11.14LIDD539 pKa = 3.35QDD541 pKa = 3.55KK542 pKa = 11.27AEE544 pKa = 4.27EE545 pKa = 4.8KK546 pKa = 10.49ILTGQPLGRR555 pKa = 11.84AVMMLDD561 pKa = 3.25ASEE564 pKa = 4.27QAFSSPLYY572 pKa = 9.67NVLSGVVSRR581 pKa = 11.84LNCEE585 pKa = 4.04RR586 pKa = 11.84RR587 pKa = 11.84SGWRR591 pKa = 11.84NAVVRR596 pKa = 11.84ASSDD600 pKa = 2.42WSRR603 pKa = 11.84FWKK606 pKa = 10.37DD607 pKa = 2.81IRR609 pKa = 11.84DD610 pKa = 3.7AKK612 pKa = 10.87CIVEE616 pKa = 4.34LDD618 pKa = 3.0WSKK621 pKa = 10.98FDD623 pKa = 4.28RR624 pKa = 11.84EE625 pKa = 4.05RR626 pKa = 11.84PRR628 pKa = 11.84ADD630 pKa = 2.79IQFCIDD636 pKa = 3.55VLCSCFRR643 pKa = 11.84PRR645 pKa = 11.84GKK647 pKa = 9.94RR648 pKa = 11.84QRR650 pKa = 11.84RR651 pKa = 11.84LLSAYY656 pKa = 9.81KK657 pKa = 10.76YY658 pKa = 8.7MLEE661 pKa = 3.75NALIHH666 pKa = 5.96KK667 pKa = 10.04VIMLDD672 pKa = 3.44DD673 pKa = 4.21GCAFEE678 pKa = 4.5YY679 pKa = 10.76EE680 pKa = 4.3GMIPSGSLWTGILGTAMNILYY701 pKa = 8.42ITAACEE707 pKa = 3.93SCGVKK712 pKa = 10.18RR713 pKa = 11.84EE714 pKa = 4.21TFVPFCAGDD723 pKa = 3.8DD724 pKa = 3.76NLTVFDD730 pKa = 4.19SAVDD734 pKa = 3.36EE735 pKa = 4.66GMLLNIRR742 pKa = 11.84VFLNNMFRR750 pKa = 11.84AGIEE754 pKa = 3.99EE755 pKa = 4.37KK756 pKa = 10.69DD757 pKa = 3.88FLIHH761 pKa = 5.79YY762 pKa = 7.48PPYY765 pKa = 10.54HH766 pKa = 4.86VTKK769 pKa = 10.02VQAVFPDD776 pKa = 4.33DD777 pKa = 4.24FDD779 pKa = 6.25LSLGTSKK786 pKa = 11.22YY787 pKa = 10.37LDD789 pKa = 3.32KK790 pKa = 11.16CVWVPFEE797 pKa = 4.73GPLLVDD803 pKa = 3.31TSRR806 pKa = 11.84GYY808 pKa = 7.59SHH810 pKa = 7.13RR811 pKa = 11.84WNYY814 pKa = 8.59VFKK817 pKa = 10.94GKK819 pKa = 9.64PKK821 pKa = 10.15FLANYY826 pKa = 8.79FLEE829 pKa = 5.02DD830 pKa = 3.3GRR832 pKa = 11.84SIRR835 pKa = 11.84PAHH838 pKa = 6.98DD839 pKa = 3.45NMEE842 pKa = 4.21KK843 pKa = 10.83LLFPEE848 pKa = 5.24NIQEE852 pKa = 4.49HH853 pKa = 6.18IEE855 pKa = 4.27DD856 pKa = 3.98YY857 pKa = 10.64EE858 pKa = 4.03AAILSMVVDD867 pKa = 4.46NPWNSHH873 pKa = 4.52NVNHH877 pKa = 6.73LMQRR881 pKa = 11.84YY882 pKa = 9.62VIVQQIKK889 pKa = 9.39RR890 pKa = 11.84QSIPPLNAAEE900 pKa = 4.34VLFCSKK906 pKa = 10.03FRR908 pKa = 11.84EE909 pKa = 4.4IADD912 pKa = 3.51NMYY915 pKa = 10.7LFPTVGYY922 pKa = 9.08WRR924 pKa = 11.84RR925 pKa = 11.84QLPGVRR931 pKa = 11.84MEE933 pKa = 5.39SIPEE937 pKa = 3.66LRR939 pKa = 11.84PIVEE943 pKa = 4.3EE944 pKa = 4.18FSQFVSGVTSLYY956 pKa = 10.64GRR958 pKa = 11.84ASSGGIDD965 pKa = 2.66AWQFLDD971 pKa = 3.51ILRR974 pKa = 11.84GDD976 pKa = 3.78RR977 pKa = 11.84DD978 pKa = 3.95LGSGQYY984 pKa = 11.27GNDD987 pKa = 3.26IEE989 pKa = 4.45KK990 pKa = 9.62WCAFLNRR997 pKa = 11.84NALSRR1002 pKa = 11.84SLRR1005 pKa = 11.84PARR1008 pKa = 11.84RR1009 pKa = 11.84FRR1011 pKa = 11.84QGTAPVEE1018 pKa = 3.86PDD1020 pKa = 2.88AKK1022 pKa = 10.63GRR1024 pKa = 11.84EE1025 pKa = 4.3SLSRR1029 pKa = 11.84LALLYY1034 pKa = 8.45EE1035 pKa = 4.56VPSSDD1040 pKa = 3.59RR1041 pKa = 11.84KK1042 pKa = 10.46DD1043 pKa = 3.42SRR1045 pKa = 11.84RR1046 pKa = 11.84LNFISRR1052 pKa = 11.84ISVMLKK1058 pKa = 9.95QFVSS1062 pKa = 3.52

Molecular weight:
121.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1457

395

1062

728.5

83.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.55 ± 0.772

2.265 ± 0.132

6.314 ± 0.757

7.001 ± 0.543

3.706 ± 0.579

5.353 ± 0.518

1.853 ± 0.165

5.148 ± 0.291

7.824 ± 1.635

10.364 ± 0.991

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.482

3.912 ± 0.556

4.53 ± 0.361

3.02 ± 0.259

8.305 ± 0.1

7.344 ± 0.499

2.951 ± 0.168

6.108 ± 0.391

1.51 ± 0.37

2.951 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski