Bacillus saganii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372LRR1|A0A372LRR1_9BACI Acetamidase OS=Bacillus saganii OX=2303992 GN=D0469_06140 PE=4 SV=1
MM1 pKa = 7.57LSITGHH7 pKa = 5.35EE8 pKa = 4.49LEE10 pKa = 5.89LINDD14 pKa = 4.09PFGILPGNRR23 pKa = 11.84FEE25 pKa = 5.75FLIDD29 pKa = 3.81LDD31 pKa = 4.02VPEE34 pKa = 5.98DD35 pKa = 3.77DD36 pKa = 4.76EE37 pKa = 5.85LYY39 pKa = 10.83SEE41 pKa = 4.26NGVYY45 pKa = 10.68LKK47 pKa = 10.68VIYY50 pKa = 10.47VLEE53 pKa = 4.46DD54 pKa = 3.37DD55 pKa = 3.88KK56 pKa = 11.89AYY58 pKa = 9.28IAKK61 pKa = 10.18YY62 pKa = 10.29DD63 pKa = 3.84FYY65 pKa = 11.53EE66 pKa = 4.49NEE68 pKa = 3.94TEE70 pKa = 4.69KK71 pKa = 11.49YY72 pKa = 9.59IDD74 pKa = 4.22LEE76 pKa = 4.25LEE78 pKa = 3.85EE79 pKa = 5.92DD80 pKa = 3.87EE81 pKa = 4.74EE82 pKa = 4.65EE83 pKa = 4.77QVNAYY88 pKa = 10.23CRR90 pKa = 11.84DD91 pKa = 3.26NLIPKK96 pKa = 10.0NEE98 pKa = 3.81

Molecular weight: 11.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372LKN0|A0A372LKN0_9BACI Uncharacterized protein OS=Bacillus saganii OX=2303992 GN=D0469_15485 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3964

0

3964

1141555

26

3093

288.0

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.435 ± 0.041

0.728 ± 0.013

4.971 ± 0.026

7.534 ± 0.047

4.479 ± 0.032

7.053 ± 0.037

2.0 ± 0.016

7.901 ± 0.038

6.943 ± 0.038

9.665 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.02

4.421 ± 0.025

3.688 ± 0.025

3.633 ± 0.031

4.199 ± 0.032

6.25 ± 0.027

5.252 ± 0.029

6.823 ± 0.031

0.945 ± 0.013

3.337 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski