Burkholderia phage phiE52237

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Tigrvirus; Burkholderia virus phi52237

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q45YF1|Q45YF1_9CAUD Putative bacteriophage membrane protein OS=Burkholderia phage phiE52237 OX=2681592 GN=BPSphi5223_0037 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.02IAALQGEE9 pKa = 4.7TLDD12 pKa = 3.91ALCWRR17 pKa = 11.84HH18 pKa = 5.61YY19 pKa = 10.73GSTAGTVEE27 pKa = 4.42AVLEE31 pKa = 4.34ANPGLAEE38 pKa = 4.5LGVVLPMGTVVEE50 pKa = 4.43MPEE53 pKa = 3.54RR54 pKa = 11.84RR55 pKa = 11.84AIEE58 pKa = 4.11TTTPLLQLFDD68 pKa = 3.82

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q45YI7|Q45YI7_9CAUD Uncharacterized protein OS=Burkholderia phage phiE52237 OX=2681592 GN=BPSphi5223_0001 PE=4 SV=1
MM1 pKa = 7.03VVHH4 pKa = 7.21DD5 pKa = 3.84ARR7 pKa = 11.84NRR9 pKa = 11.84VRR11 pKa = 11.84MHH13 pKa = 6.76PPPRR17 pKa = 11.84TAGRR21 pKa = 11.84KK22 pKa = 8.96DD23 pKa = 3.33RR24 pKa = 11.84SSVGSRR30 pKa = 11.84GCRR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 3.7RR36 pKa = 11.84LAQTAIRR43 pKa = 11.84KK44 pKa = 8.07NRR46 pKa = 11.84WEE48 pKa = 3.47MRR50 pKa = 11.84FYY52 pKa = 10.93KK53 pKa = 10.08RR54 pKa = 11.84QKK56 pKa = 9.85NGPWWFDD63 pKa = 3.05LSIDD67 pKa = 3.7GSRR70 pKa = 11.84IRR72 pKa = 11.84QSSGTSDD79 pKa = 2.75RR80 pKa = 11.84RR81 pKa = 11.84AAEE84 pKa = 3.71EE85 pKa = 3.76LAAKK89 pKa = 9.58VASDD93 pKa = 2.99YY94 pKa = 10.33WRR96 pKa = 11.84QKK98 pKa = 11.15KK99 pKa = 10.23LGEE102 pKa = 4.24RR103 pKa = 11.84PAVTWDD109 pKa = 3.13AAVVHH114 pKa = 6.13WLKK117 pKa = 10.89QNQHH121 pKa = 4.62QRR123 pKa = 11.84SLEE126 pKa = 4.1TTKK129 pKa = 10.4QRR131 pKa = 11.84LRR133 pKa = 11.84WLTDD137 pKa = 3.02QLKK140 pKa = 10.66GEE142 pKa = 4.22NVRR145 pKa = 11.84NIDD148 pKa = 3.52RR149 pKa = 11.84EE150 pKa = 4.15RR151 pKa = 11.84IQALIEE157 pKa = 3.81TKK159 pKa = 10.45AAEE162 pKa = 4.5KK163 pKa = 10.88YY164 pKa = 10.03RR165 pKa = 11.84GSPVAGATVNRR176 pKa = 11.84HH177 pKa = 4.03MAALSVILHH186 pKa = 6.28HH187 pKa = 6.37CHH189 pKa = 6.46AEE191 pKa = 3.93GWIDD195 pKa = 3.67AVPPIRR201 pKa = 11.84KK202 pKa = 8.83LRR204 pKa = 11.84EE205 pKa = 3.33NSARR209 pKa = 11.84LTWLTRR215 pKa = 11.84AQAQRR220 pKa = 11.84LLEE223 pKa = 4.15EE224 pKa = 4.95LPTHH228 pKa = 6.55LRR230 pKa = 11.84QMARR234 pKa = 11.84FALATGLRR242 pKa = 11.84EE243 pKa = 4.27SNVRR247 pKa = 11.84LLEE250 pKa = 3.9WAQVDD255 pKa = 4.41RR256 pKa = 11.84EE257 pKa = 4.03RR258 pKa = 11.84ALAWIHH264 pKa = 6.96ADD266 pKa = 3.13QAKK269 pKa = 9.28AGKK272 pKa = 9.85VISVPLNEE280 pKa = 4.97DD281 pKa = 2.97ALGVLRR287 pKa = 11.84EE288 pKa = 3.99QQGQHH293 pKa = 4.65KK294 pKa = 10.04RR295 pKa = 11.84YY296 pKa = 8.69VFVYY300 pKa = 9.12KK301 pKa = 10.36GAPIGRR307 pKa = 11.84IYY309 pKa = 10.26NHH311 pKa = 6.83AWQKK315 pKa = 9.5ACVRR319 pKa = 11.84AGLSGLRR326 pKa = 11.84FHH328 pKa = 7.58DD329 pKa = 5.35LRR331 pKa = 11.84HH332 pKa = 5.71TWASWHH338 pKa = 5.46VQAGTPLPILQQLGGWASYY357 pKa = 10.9QMVLRR362 pKa = 11.84YY363 pKa = 9.69AHH365 pKa = 7.51LGRR368 pKa = 11.84DD369 pKa = 3.49HH370 pKa = 6.31VAAYY374 pKa = 10.25ADD376 pKa = 3.74NIGTLRR382 pKa = 11.84HH383 pKa = 6.08KK384 pKa = 10.73SGTPPEE390 pKa = 4.1IEE392 pKa = 4.42KK393 pKa = 11.01GSDD396 pKa = 3.24CSEE399 pKa = 4.09PLSNLVAWGGIEE411 pKa = 3.84PPTRR415 pKa = 11.84GFSKK419 pKa = 9.85RR420 pKa = 11.84TADD423 pKa = 3.31TSAPSGKK430 pKa = 10.14ASRR433 pKa = 11.84PPKK436 pKa = 9.56KK437 pKa = 10.13RR438 pKa = 11.84KK439 pKa = 9.06AAA441 pKa = 3.76

Molecular weight: 50.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

11038

65

988

234.9

25.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.526 ± 0.526

0.843 ± 0.134

6.161 ± 0.385

5.2 ± 0.265

3.28 ± 0.254

7.9 ± 0.525

2.482 ± 0.204

3.95 ± 0.221

4.05 ± 0.311

8.942 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.187

2.935 ± 0.182

4.765 ± 0.373

3.434 ± 0.267

8.462 ± 0.507

5.064 ± 0.226

5.862 ± 0.305

6.777 ± 0.283

1.54 ± 0.171

2.473 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski