Clostridium sulfidigenes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084J775|A0A084J775_9CLOT 50S ribosomal protein L35 OS=Clostridium sulfidigenes OX=318464 GN=rpmI PE=3 SV=1
MM1 pKa = 7.31MKK3 pKa = 10.5YY4 pKa = 10.5CIFDD8 pKa = 3.59EE9 pKa = 4.76KK10 pKa = 10.81KK11 pKa = 9.7VCNDD15 pKa = 3.12CGEE18 pKa = 4.64CNVCEE23 pKa = 4.0YY24 pKa = 10.94NRR26 pKa = 11.84DD27 pKa = 4.13KK28 pKa = 11.24ICDD31 pKa = 3.51NCGKK35 pKa = 9.99CLEE38 pKa = 4.2IEE40 pKa = 4.72GYY42 pKa = 9.34DD43 pKa = 3.6VKK45 pKa = 10.88AVKK48 pKa = 9.64IDD50 pKa = 3.52EE51 pKa = 4.3VFEE54 pKa = 4.38KK55 pKa = 10.96NKK57 pKa = 10.5EE58 pKa = 3.98EE59 pKa = 4.22EE60 pKa = 4.07IKK62 pKa = 10.24IDD64 pKa = 4.74LEE66 pKa = 4.36DD67 pKa = 4.9FSDD70 pKa = 3.84FDD72 pKa = 4.37TIEE75 pKa = 4.5EE76 pKa = 3.98EE77 pKa = 5.95DD78 pKa = 3.99SFQEE82 pKa = 4.62DD83 pKa = 3.65IVPLEE88 pKa = 4.24EE89 pKa = 6.07DD90 pKa = 4.01EE91 pKa = 4.84IPYY94 pKa = 10.01IDD96 pKa = 5.25ALDD99 pKa = 5.01DD100 pKa = 3.89EE101 pKa = 5.93SNWDD105 pKa = 3.81YY106 pKa = 11.46ISDD109 pKa = 3.95VEE111 pKa = 5.29GMDD114 pKa = 3.39EE115 pKa = 4.65LMEE118 pKa = 4.14EE119 pKa = 4.28TGTDD123 pKa = 3.43GLLHH127 pKa = 6.52EE128 pKa = 5.82EE129 pKa = 4.41YY130 pKa = 10.22PGLYY134 pKa = 9.51VVNNKK139 pKa = 8.6RR140 pKa = 3.26

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084JDG4|A0A084JDG4_9CLOT DNA alkylation repair protein OS=Clostridium sulfidigenes OX=318464 GN=IO99_07035 PE=4 SV=1
MM1 pKa = 7.86AYY3 pKa = 10.31DD4 pKa = 4.45RR5 pKa = 11.84NDD7 pKa = 3.16KK8 pKa = 10.59NDD10 pKa = 3.01RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 10.43GGNRR17 pKa = 11.84KK18 pKa = 8.12RR19 pKa = 11.84AKK21 pKa = 10.06RR22 pKa = 11.84KK23 pKa = 9.17VCAFCMDD30 pKa = 3.58KK31 pKa = 10.44ATSIDD36 pKa = 3.52YY37 pKa = 11.03KK38 pKa = 10.97DD39 pKa = 3.22INKK42 pKa = 9.06LRR44 pKa = 11.84KK45 pKa = 9.22YY46 pKa = 8.79VTEE49 pKa = 4.26KK50 pKa = 11.15GKK52 pKa = 10.12ILPRR56 pKa = 11.84RR57 pKa = 11.84ISGNCAKK64 pKa = 9.56HH65 pKa = 5.6QRR67 pKa = 11.84MLTVAIKK74 pKa = 10.13RR75 pKa = 11.84ARR77 pKa = 11.84NIALLPFTTEE87 pKa = 3.59

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3148

0

3148

922629

31

1797

293.1

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.505 ± 0.048

1.29 ± 0.018

5.458 ± 0.034

7.678 ± 0.054

4.362 ± 0.038

6.567 ± 0.044

1.42 ± 0.014

10.143 ± 0.054

8.742 ± 0.043

8.985 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.023

6.1 ± 0.044

2.748 ± 0.024

2.207 ± 0.02

3.438 ± 0.03

6.301 ± 0.033

4.915 ± 0.029

6.615 ± 0.043

0.671 ± 0.013

4.132 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski