Hymenobacter sedentarius

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4BBH7|A0A0U4BBH7_9BACT Magnesium chelatase OS=Hymenobacter sedentarius OX=1411621 GN=AUC43_01825 PE=3 SV=1
MM1 pKa = 8.3DD2 pKa = 4.51YY3 pKa = 11.16FNDD6 pKa = 3.78PEE8 pKa = 4.99LNGKK12 pKa = 8.58YY13 pKa = 10.13LGTITKK19 pKa = 10.22DD20 pKa = 3.39FVVVCDD26 pKa = 3.96TLLEE30 pKa = 4.3ASSQIRR36 pKa = 11.84KK37 pKa = 9.92GDD39 pKa = 3.5FSKK42 pKa = 11.4YY43 pKa = 9.68PIFVFAKK50 pKa = 10.18QEE52 pKa = 4.02VPLGGLLVNADD63 pKa = 3.87EE64 pKa = 6.18LNLEE68 pKa = 3.95WHH70 pKa = 6.16VFASYY75 pKa = 10.26MEE77 pKa = 5.02LFVQQGIIDD86 pKa = 3.84VDD88 pKa = 3.8GVEE91 pKa = 4.87AFQATYY97 pKa = 10.81KK98 pKa = 11.19DD99 pKa = 3.67PDD101 pKa = 3.65EE102 pKa = 4.65FCCLFVLDD110 pKa = 4.71EE111 pKa = 4.43EE112 pKa = 4.58FTNFVFVPYY121 pKa = 10.49PEE123 pKa = 4.62DD124 pKa = 3.2

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3SKX9|A0A0U3SKX9_9BACT Uncharacterized protein OS=Hymenobacter sedentarius OX=1411621 GN=AUC43_17980 PE=4 SV=1
MM1 pKa = 7.44SLFGWSSLLALLPRR15 pKa = 11.84NTPEE19 pKa = 3.36VRR21 pKa = 11.84PRR23 pKa = 11.84SGQALSAYY31 pKa = 9.42PPEE34 pKa = 4.45TAGATTPALATVGLKK49 pKa = 10.09VALLRR54 pKa = 11.84CQSTHH59 pKa = 5.95RR60 pKa = 11.84LIIGHH65 pKa = 6.61PCRR68 pKa = 11.84PAPPLSRR75 pKa = 11.84QIASEE80 pKa = 4.18SEE82 pKa = 3.88APQWRR87 pKa = 11.84VACRR91 pKa = 11.84FATRR95 pKa = 11.84PRR97 pKa = 11.84RR98 pKa = 11.84SCAQATARR106 pKa = 11.84AFSARR111 pKa = 11.84GLSFCDD117 pKa = 4.4AIDD120 pKa = 3.52SKK122 pKa = 11.33GFEE125 pKa = 4.43WPACSPKK132 pKa = 10.15KK133 pKa = 10.35ACIYY137 pKa = 6.96QQRR140 pKa = 11.84PMRR143 pKa = 11.84LAALLLRR150 pKa = 11.84PDD152 pKa = 3.72SAQATMPAIAKK163 pKa = 9.61GEE165 pKa = 3.91PAPEE169 pKa = 3.66FRR171 pKa = 11.84SLHH174 pKa = 5.94LRR176 pKa = 11.84HH177 pKa = 6.3SMPLHH182 pKa = 5.35VRR184 pKa = 11.84YY185 pKa = 7.92ATCAVAAGQPRR196 pKa = 11.84LYY198 pKa = 10.45PEE200 pKa = 4.01AA201 pKa = 5.92

Molecular weight: 21.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3828

0

3828

1334700

38

3966

348.7

38.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.033 ± 0.058

0.709 ± 0.013

4.893 ± 0.031

5.131 ± 0.04

4.089 ± 0.024

7.814 ± 0.038

2.137 ± 0.022

4.264 ± 0.031

4.078 ± 0.043

10.801 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.019

3.818 ± 0.033

5.328 ± 0.031

4.44 ± 0.03

5.966 ± 0.037

5.407 ± 0.034

6.301 ± 0.075

7.218 ± 0.03

1.222 ± 0.017

3.448 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski