Geminocystis sp. NIES-3708

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Chroococcaceae; Geminocystis; unclassified Geminocystis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6AHQ5|A0A0D6AHQ5_9CHRO Uncharacterized protein OS=Geminocystis sp. NIES-3708 OX=1615909 GN=GM3708_2744 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 4.04VKK4 pKa = 11.11NSVNIEE10 pKa = 4.05SPIATTKK17 pKa = 8.14VTPVSADD24 pKa = 3.43TSEE27 pKa = 4.04LTADD31 pKa = 4.53LSQKK35 pKa = 10.58LSLPEE40 pKa = 3.89VHH42 pKa = 7.05SSSTPLIKK50 pKa = 10.6NSLSSIISRR59 pKa = 11.84GISPPEE65 pKa = 3.79TVIKK69 pKa = 10.53LQDD72 pKa = 3.35SSPTFFSPIKK82 pKa = 9.28PLQAISAPSGDD93 pKa = 3.63YY94 pKa = 10.82RR95 pKa = 11.84IDD97 pKa = 3.56ALLGGNKK104 pKa = 8.99WGITNLTYY112 pKa = 10.78SFYY115 pKa = 11.15AGGSFYY121 pKa = 11.01GSEE124 pKa = 4.02TGLASVSEE132 pKa = 4.1AVKK135 pKa = 11.08NNVRR139 pKa = 11.84YY140 pKa = 9.78ILNNLIAPLINLTFTEE156 pKa = 4.7VTDD159 pKa = 3.71SPSNYY164 pKa = 9.29GQLRR168 pKa = 11.84YY169 pKa = 9.86LLSTDD174 pKa = 2.96PGYY177 pKa = 11.34AYY179 pKa = 10.27AYY181 pKa = 10.37YY182 pKa = 10.14PFSTDD187 pKa = 2.99TNQGNGNDD195 pKa = 3.51VAGDD199 pKa = 3.49VFLNPDD205 pKa = 3.31YY206 pKa = 11.66DD207 pKa = 4.47NNFDD211 pKa = 3.94TNGFRR216 pKa = 11.84GGAGTHH222 pKa = 7.06GYY224 pKa = 10.09QSLIHH229 pKa = 5.93EE230 pKa = 4.66TLHH233 pKa = 6.8ALGIKK238 pKa = 10.33HH239 pKa = 6.3SGNYY243 pKa = 8.97NGSGAGDD250 pKa = 4.05PPYY253 pKa = 10.79LPYY256 pKa = 11.04GEE258 pKa = 5.24EE259 pKa = 3.89NWDD262 pKa = 3.75NTLMTYY268 pKa = 10.75NFDD271 pKa = 4.14SGDD274 pKa = 3.75EE275 pKa = 4.17PSTPMAYY282 pKa = 10.1DD283 pKa = 3.71ILALQYY289 pKa = 11.03LYY291 pKa = 10.97GAKK294 pKa = 9.98SYY296 pKa = 11.33NSNNTIYY303 pKa = 10.29TFSDD307 pKa = 2.92TDD309 pKa = 4.01LYY311 pKa = 11.54SDD313 pKa = 3.94GSKK316 pKa = 9.33TVGSSTLNNKK326 pKa = 7.31ITIWDD331 pKa = 3.7SGGTDD336 pKa = 2.81ILNFTGLGSNTGGYY350 pKa = 9.63RR351 pKa = 11.84FDD353 pKa = 4.14MNPGGWFSTQTAFNGTGYY371 pKa = 10.62NVDD374 pKa = 3.86PGPPVSFSSGTTYY387 pKa = 10.92RR388 pKa = 11.84ATTNGTRR395 pKa = 11.84LGYY398 pKa = 10.56GIIIEE403 pKa = 4.34HH404 pKa = 6.67LVNSSSNDD412 pKa = 3.39YY413 pKa = 10.78IIANSAANQFSGYY426 pKa = 10.65GVITSTGNDD435 pKa = 3.54TIEE438 pKa = 4.82GGNSLDD444 pKa = 3.76TLDD447 pKa = 4.67LSEE450 pKa = 4.53FTSSSVSQIQSGNNLVLGLGSARR473 pKa = 11.84SITVKK478 pKa = 10.42DD479 pKa = 3.91YY480 pKa = 11.35YY481 pKa = 10.44LTSTDD486 pKa = 3.3RR487 pKa = 11.84LNIQFSSTNILTSFAIAATNANQTEE512 pKa = 4.72GNSGTKK518 pKa = 10.55AFTFTINRR526 pKa = 11.84SGTTTGANNVNWAVTGSGTNPANATDD552 pKa = 4.05FSGGVLPSGTVSFAANEE569 pKa = 4.05TSKK572 pKa = 11.28VITVNVQGDD581 pKa = 4.02TTVEE585 pKa = 3.62PDD587 pKa = 3.19EE588 pKa = 4.78NFTVTLSNATNGATITTATATGTILNDD615 pKa = 4.0DD616 pKa = 4.12GTNGNTFSNTSTISIPEE633 pKa = 3.9IGPASSYY640 pKa = 10.69PSTINVSGLSGNISKK655 pKa = 8.1VTVTLNNLSHH665 pKa = 7.06TFPDD669 pKa = 4.29DD670 pKa = 3.91LDD672 pKa = 4.16ILLVSPTGAKK682 pKa = 8.67TLLMSDD688 pKa = 3.3VGEE691 pKa = 4.58SNDD694 pKa = 4.1LSDD697 pKa = 3.55VTLTFDD703 pKa = 3.31PTATNFLPDD712 pKa = 3.46SGLITSGTYY721 pKa = 10.22KK722 pKa = 9.72PTDD725 pKa = 3.79FEE727 pKa = 5.59SDD729 pKa = 3.73DD730 pKa = 4.38VFDD733 pKa = 4.31SPAPVGPYY741 pKa = 10.04NADD744 pKa = 2.87LSVFNNTNPNGEE756 pKa = 3.64WRR758 pKa = 11.84LFVVDD763 pKa = 5.95DD764 pKa = 3.74ISGDD768 pKa = 3.49FGSIAGGLSLTFEE781 pKa = 4.54TTASFPTTLAIAATNANQTEE801 pKa = 4.72GNSGTKK807 pKa = 10.46AFTFTVTRR815 pKa = 11.84SGTTTGANNVNWAVTGSGTNPANATDD841 pKa = 4.05FSGGVLPSGTVSFAANEE858 pKa = 4.05TSKK861 pKa = 11.28VITVNVQGDD870 pKa = 4.02TTVEE874 pKa = 3.62PDD876 pKa = 3.19EE877 pKa = 4.78NFTVTLSNATNGATITTATAIGTIQNDD904 pKa = 3.71DD905 pKa = 3.67TPIPTNLAIAATNANQTEE923 pKa = 4.72GNSGTKK929 pKa = 10.46AFTFTVTRR937 pKa = 11.84SGTTTGANNVNWAVTGSGTNPANATDD963 pKa = 4.05FSGGVLPSGTVSFAANEE980 pKa = 4.05TSKK983 pKa = 11.28VITVNVQGDD992 pKa = 4.02TTVEE996 pKa = 3.62PDD998 pKa = 3.19EE999 pKa = 4.78NFTVTLSNATNGATITTATAIGTIQNDD1026 pKa = 3.7DD1027 pKa = 3.9TNTFLPQISISPNQTVIEE1045 pKa = 4.51GLTSLQNVTYY1055 pKa = 9.51TVILSQASSQTVTVQYY1071 pKa = 9.68ATTNGTAIAGSDD1083 pKa = 3.5YY1084 pKa = 10.42TATSGTLTFSPGQISKK1100 pKa = 9.28TIILPILNDD1109 pKa = 3.7FVNEE1113 pKa = 3.57FDD1115 pKa = 4.37EE1116 pKa = 4.8NFNLTLSNSTNANLSNSGVTTTIADD1141 pKa = 3.89TLFTAVTTTLSANVEE1156 pKa = 4.04NLILTGITAINGTGNGGNNIISGNTANNNLNGGAGNDD1193 pKa = 3.86TLNDD1197 pKa = 3.51GDD1199 pKa = 5.48GNDD1202 pKa = 4.26TLDD1205 pKa = 3.89GGAGSDD1211 pKa = 3.76VMSGGLGNDD1220 pKa = 4.16FYY1222 pKa = 11.6YY1223 pKa = 11.27VNSTVDD1229 pKa = 3.38VVVEE1233 pKa = 4.06NASQGTDD1240 pKa = 3.14TVISSITHH1248 pKa = 5.34TLAANVEE1255 pKa = 4.22NLTLIDD1261 pKa = 3.59ISAINGTGNTLNNTITGNIANNNLNGGDD1289 pKa = 4.06GNDD1292 pKa = 3.73TLTGGDD1298 pKa = 4.5GNDD1301 pKa = 3.66TLNGGTGSDD1310 pKa = 3.65RR1311 pKa = 11.84LTGGTGNDD1319 pKa = 3.86LYY1321 pKa = 11.51LVDD1324 pKa = 4.05NVGDD1328 pKa = 3.88IVSEE1332 pKa = 4.18TSTLATEE1339 pKa = 4.29IDD1341 pKa = 4.11TVQSSITYY1349 pKa = 8.19TLTTNVEE1356 pKa = 4.04NLTLTGTSAINGTGNTLNNTITGNIANNNLNGGDD1390 pKa = 4.06GNDD1393 pKa = 3.73TLTGGDD1399 pKa = 4.5GNDD1402 pKa = 3.82TLNGSIGSDD1411 pKa = 2.86RR1412 pKa = 11.84LTGGTGNDD1420 pKa = 3.8LYY1422 pKa = 10.73IVDD1425 pKa = 4.13NLGDD1429 pKa = 3.75IVIEE1433 pKa = 4.14TSTLATEE1440 pKa = 4.29IDD1442 pKa = 4.11TVQSLITYY1450 pKa = 7.52TLTTNVEE1457 pKa = 4.04NLTLTGTSAINGTGNILNNTITGNIANNNLNGGDD1491 pKa = 4.06GNDD1494 pKa = 3.73TLTGGDD1500 pKa = 4.5GNDD1503 pKa = 3.66TLNGGTGSDD1512 pKa = 3.57RR1513 pKa = 11.84LTSGTGNDD1521 pKa = 4.28LYY1523 pKa = 10.73IVDD1526 pKa = 4.08NVGDD1530 pKa = 3.96IVIEE1534 pKa = 4.12TSTLATEE1541 pKa = 4.29IDD1543 pKa = 4.11TVQSSITYY1551 pKa = 8.19TLTTNVEE1558 pKa = 4.04NLTLTGTSAINGTGNTLNNTITGNIANNNLNGGDD1592 pKa = 4.06GNDD1595 pKa = 3.73TLTGGDD1601 pKa = 4.5GNDD1604 pKa = 3.66TLNGGTGSDD1613 pKa = 3.65RR1614 pKa = 11.84LTGGTGNDD1622 pKa = 3.82LYY1624 pKa = 10.77IVDD1627 pKa = 4.07NVGDD1631 pKa = 4.01IVSEE1635 pKa = 4.18TSTLATEE1642 pKa = 4.29IDD1644 pKa = 4.11TVQSSITYY1652 pKa = 8.19TLTTNVEE1659 pKa = 4.04NLTLTGTTAINGTGNTLNNTITGNTANNSLDD1690 pKa = 3.41GGAGNDD1696 pKa = 3.81TLNGGTGIDD1705 pKa = 3.78SLIGGTGNDD1714 pKa = 4.13LYY1716 pKa = 10.77IVDD1719 pKa = 4.07NVGDD1723 pKa = 4.01IVSEE1727 pKa = 4.18TSTLATEE1734 pKa = 4.29IDD1736 pKa = 4.11TVQSSITYY1744 pKa = 8.16TLTDD1748 pKa = 3.28NVEE1751 pKa = 3.93NLTLTGTTAINGTGNTLKK1769 pKa = 10.87NVITGNSGNNSLSGGDD1785 pKa = 4.19GNDD1788 pKa = 3.4TLIGGDD1794 pKa = 4.26GNDD1797 pKa = 3.55TLIGGGSNDD1806 pKa = 3.71TLTGGNGLDD1815 pKa = 3.18SFRR1818 pKa = 11.84FNSASEE1824 pKa = 4.15GVDD1827 pKa = 4.3FITDD1831 pKa = 3.66FTVADD1836 pKa = 3.5DD1837 pKa = 4.75TIRR1840 pKa = 11.84VLGSAFGGGLVAGTLSISQFTIGSSATTSSHH1871 pKa = 6.01RR1872 pKa = 11.84FFYY1875 pKa = 10.82NSSNGGLFFDD1885 pKa = 4.46VDD1887 pKa = 4.08GNGATSAVQFATLNISLTMTNADD1910 pKa = 2.73IVVII1914 pKa = 4.28

Molecular weight: 196.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6AF49|A0A0D6AF49_9CHRO Uncharacterized protein OS=Geminocystis sp. NIES-3708 OX=1615909 GN=GM3708_1761 PE=4 SV=1
MM1 pKa = 7.64SKK3 pKa = 8.22HH4 pKa = 5.3TLNGTRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.04QRR14 pKa = 11.84RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TEE26 pKa = 3.49NGRR29 pKa = 11.84KK30 pKa = 9.26VIQARR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 8.9GRR40 pKa = 11.84KK41 pKa = 8.69RR42 pKa = 11.84LSVV45 pKa = 3.2

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3607

0

3607

1128004

37

4420

312.7

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.113 ± 0.051

0.95 ± 0.014

5.117 ± 0.038

6.574 ± 0.052

4.368 ± 0.029

6.166 ± 0.063

1.698 ± 0.023

8.826 ± 0.045

6.493 ± 0.052

10.771 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.802 ± 0.021

6.267 ± 0.067

3.915 ± 0.028

4.704 ± 0.029

3.867 ± 0.038

6.491 ± 0.036

5.549 ± 0.057

5.627 ± 0.042

1.269 ± 0.019

3.433 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski