Desulfobacteraceae bacterium SEEP-SAG10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; unclassified Desulfobacteraceae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6HIT2|A0A2P6HIT2_9DELT Uncharacterized protein OS=Desulfobacteraceae bacterium SEEP-SAG10 OX=2100160 GN=C6A36_00030 PE=4 SV=1
MM1 pKa = 6.82NTSKK5 pKa = 11.09KK6 pKa = 9.52EE7 pKa = 3.94DD8 pKa = 3.09CKK10 pKa = 10.8MFYY13 pKa = 10.56EE14 pKa = 5.02KK15 pKa = 10.96YY16 pKa = 10.28KK17 pKa = 11.12NGDD20 pKa = 3.0NFLINRR26 pKa = 11.84LKK28 pKa = 10.81IMFQFIVIVVLLTSYY43 pKa = 10.98NNSYY47 pKa = 10.35GKK49 pKa = 9.56IWTYY53 pKa = 11.01RR54 pKa = 11.84QITDD58 pKa = 3.54NDD60 pKa = 3.63IKK62 pKa = 11.0DD63 pKa = 3.65SSVSIDD69 pKa = 3.13NGQIAWRR76 pKa = 11.84GYY78 pKa = 9.91GEE80 pKa = 4.05NGKK83 pKa = 9.28LAIFFMDD90 pKa = 3.77LNEE93 pKa = 4.25GVKK96 pKa = 10.65EE97 pKa = 3.9NLTADD102 pKa = 3.26KK103 pKa = 10.9VYY105 pKa = 10.65GYY107 pKa = 10.39FGTPQISNGQVTWRR121 pKa = 11.84RR122 pKa = 11.84YY123 pKa = 10.49DD124 pKa = 3.27GDD126 pKa = 3.87NNKK129 pKa = 10.59DD130 pKa = 4.93SILLWDD136 pKa = 4.52GQDD139 pKa = 2.74IHH141 pKa = 8.18IVSEE145 pKa = 4.27YY146 pKa = 11.0DD147 pKa = 3.62CGDD150 pKa = 3.57FPYY153 pKa = 10.35QGYY156 pKa = 8.04PPSYY160 pKa = 10.65GSLEE164 pKa = 4.1PSLHH168 pKa = 6.23NGQIAYY174 pKa = 9.41AAWDD178 pKa = 3.85GNDD181 pKa = 3.26YY182 pKa = 11.1EE183 pKa = 4.25IFYY186 pKa = 10.72WNGTEE191 pKa = 5.31IIQITDD197 pKa = 3.13NLTNDD202 pKa = 3.85YY203 pKa = 9.4EE204 pKa = 4.26AQLWNGTISYY214 pKa = 8.79TGYY217 pKa = 10.23KK218 pKa = 10.17DD219 pKa = 3.7EE220 pKa = 5.08SAPSDD225 pKa = 3.01IFYY228 pKa = 10.31WDD230 pKa = 3.13GTEE233 pKa = 3.89IQNISDD239 pKa = 3.75KK240 pKa = 11.47AGTCEE245 pKa = 4.15DD246 pKa = 3.18SHH248 pKa = 8.19LRR250 pKa = 11.84EE251 pKa = 4.25SKK253 pKa = 10.06IVHH256 pKa = 5.66SAYY259 pKa = 10.36GLSGEE264 pKa = 4.57YY265 pKa = 9.65GTDD268 pKa = 2.65IYY270 pKa = 10.77IWDD273 pKa = 4.4GSQDD277 pKa = 3.06RR278 pKa = 11.84CLIPVIGYY286 pKa = 9.74DD287 pKa = 3.64YY288 pKa = 10.61EE289 pKa = 4.37PEE291 pKa = 3.91IFEE294 pKa = 6.34SLICWTGWPYY304 pKa = 11.33GKK306 pKa = 9.63PKK308 pKa = 10.04GPCVGWVWDD317 pKa = 4.48GYY319 pKa = 10.72KK320 pKa = 10.19LHH322 pKa = 6.8NVMEE326 pKa = 4.48SPYY329 pKa = 10.4NVSFPRR335 pKa = 11.84ADD337 pKa = 3.13GSMIAFTAHH346 pKa = 7.28DD347 pKa = 4.55GNDD350 pKa = 3.47DD351 pKa = 4.11EE352 pKa = 6.02IFIAEE357 pKa = 4.82YY358 pKa = 8.96ITGTYY363 pKa = 10.23KK364 pKa = 10.66PVTPAGEE371 pKa = 4.17TLNILLEE378 pKa = 4.26NEE380 pKa = 3.98LAYY383 pKa = 10.48VANGEE388 pKa = 4.09GDD390 pKa = 4.07LKK392 pKa = 10.87IVDD395 pKa = 4.26ISDD398 pKa = 3.17EE399 pKa = 4.29GNPVEE404 pKa = 5.27VGGLYY409 pKa = 10.04IPDD412 pKa = 3.79ACSGFEE418 pKa = 3.85VQKK421 pKa = 10.13RR422 pKa = 11.84GNYY425 pKa = 8.61IFLANRR431 pKa = 11.84CGGVKK436 pKa = 9.92IIDD439 pKa = 3.81VTDD442 pKa = 3.52GTNPILVSTVEE453 pKa = 4.21TPDD456 pKa = 3.23MATHH460 pKa = 6.25ITLDD464 pKa = 3.39GNYY467 pKa = 10.25LYY469 pKa = 10.42IGDD472 pKa = 4.18RR473 pKa = 11.84LGGLRR478 pKa = 11.84ILDD481 pKa = 3.47VTIPEE486 pKa = 4.47SVNEE490 pKa = 4.05VGNLDD495 pKa = 4.95LEE497 pKa = 4.51GTTYY501 pKa = 11.17GIAIKK506 pKa = 10.97DD507 pKa = 3.46NFAYY511 pKa = 10.3VANDD515 pKa = 3.17YY516 pKa = 11.51DD517 pKa = 4.51GIQVVDD523 pKa = 3.69ISDD526 pKa = 4.05PEE528 pKa = 4.38SPGMVQRR535 pKa = 11.84YY536 pKa = 8.72DD537 pKa = 3.41LSGITVWDD545 pKa = 3.48IKK547 pKa = 10.9IHH549 pKa = 7.36DD550 pKa = 4.57DD551 pKa = 3.39YY552 pKa = 11.82AYY554 pKa = 10.36LVCPGYY560 pKa = 10.47GIKK563 pKa = 10.53VLDD566 pKa = 3.75ISDD569 pKa = 4.42PYY571 pKa = 11.05DD572 pKa = 3.36LRR574 pKa = 11.84EE575 pKa = 4.09IGEE578 pKa = 4.54CVLPDD583 pKa = 4.03GSRR586 pKa = 11.84PDD588 pKa = 3.84HH589 pKa = 6.5FLPPLDD595 pKa = 3.38ISFVDD600 pKa = 4.17HH601 pKa = 6.23YY602 pKa = 11.81AFVGHH607 pKa = 6.62GNDD610 pKa = 3.61GALVLDD616 pKa = 4.54ISNPYY621 pKa = 8.91YY622 pKa = 9.83PRR624 pKa = 11.84IVEE627 pKa = 4.94RR628 pKa = 11.84IDD630 pKa = 3.28TPGYY634 pKa = 8.7AWGMSIRR641 pKa = 11.84GKK643 pKa = 10.32YY644 pKa = 10.24LYY646 pKa = 10.68VADD649 pKa = 4.61GSEE652 pKa = 4.25GFQIINISDD661 pKa = 3.62YY662 pKa = 10.59MQTLYY667 pKa = 11.11EE668 pKa = 4.33DD669 pKa = 4.79AEE671 pKa = 4.7DD672 pKa = 4.06GRR674 pKa = 11.84ISGWDD679 pKa = 3.16IYY681 pKa = 11.66DD682 pKa = 3.76NSPDD686 pKa = 3.57GAQVTNGFDD695 pKa = 3.47EE696 pKa = 4.91DD697 pKa = 3.68RR698 pKa = 11.84QSRR701 pKa = 11.84VIHH704 pKa = 6.06LNGSGHH710 pKa = 6.51LNGYY714 pKa = 7.64RR715 pKa = 11.84LRR717 pKa = 11.84SDD719 pKa = 5.69DD720 pKa = 3.29GSEE723 pKa = 3.76WHH725 pKa = 6.69NSSQFVIEE733 pKa = 3.87WSMKK737 pKa = 8.06YY738 pKa = 10.32SEE740 pKa = 4.82FFTVYY745 pKa = 10.79VDD747 pKa = 3.55IEE749 pKa = 4.32TTAGQRR755 pKa = 11.84YY756 pKa = 7.02MVYY759 pKa = 10.41KK760 pKa = 10.51PVDD763 pKa = 3.89FDD765 pKa = 4.29DD766 pKa = 5.66LGDD769 pKa = 3.79GGYY772 pKa = 10.23VYY774 pKa = 10.55HH775 pKa = 7.15GLGSDD780 pKa = 3.7TIDD783 pKa = 4.09GQWHH787 pKa = 5.59TFVHH791 pKa = 7.13DD792 pKa = 4.08LQTDD796 pKa = 3.4IEE798 pKa = 4.29EE799 pKa = 4.46AQPGVTILEE808 pKa = 4.23VNGFYY813 pKa = 10.17IRR815 pKa = 11.84GSGSVDD821 pKa = 4.19DD822 pKa = 4.75IGLMDD827 pKa = 4.3TMLINQDD834 pKa = 3.34TDD836 pKa = 3.22SDD838 pKa = 4.81GITDD842 pKa = 4.04FNEE845 pKa = 3.76TNIYY849 pKa = 8.55GTDD852 pKa = 3.62PNKK855 pKa = 10.85ADD857 pKa = 3.74TDD859 pKa = 3.57EE860 pKa = 5.97DD861 pKa = 4.77GIIDD865 pKa = 4.4GDD867 pKa = 3.89EE868 pKa = 3.73LSYY871 pKa = 10.53WGLNWDD877 pKa = 3.88EE878 pKa = 6.03DD879 pKa = 4.49YY880 pKa = 11.69DD881 pKa = 4.42GDD883 pKa = 4.77NLINLLDD890 pKa = 4.64PDD892 pKa = 4.33ADD894 pKa = 3.61NDD896 pKa = 3.88GFLDD900 pKa = 3.97GEE902 pKa = 4.4EE903 pKa = 4.12IAAGSDD909 pKa = 3.4PADD912 pKa = 4.18PEE914 pKa = 4.38SSPLSLVVYY923 pKa = 10.08EE924 pKa = 4.66DD925 pKa = 4.9AEE927 pKa = 4.44DD928 pKa = 3.64SSIIGWDD935 pKa = 3.65VYY937 pKa = 11.73DD938 pKa = 5.6DD939 pKa = 4.93DD940 pKa = 5.49PPGALISNVYY950 pKa = 10.26DD951 pKa = 3.48EE952 pKa = 4.88NRR954 pKa = 11.84QSRR957 pKa = 11.84VIEE960 pKa = 4.11FSGSGVTNGYY970 pKa = 9.16RR971 pKa = 11.84LRR973 pKa = 11.84DD974 pKa = 3.49QNGAPWHH981 pKa = 5.28NTRR984 pKa = 11.84QFVIEE989 pKa = 4.0WSMKK993 pKa = 9.71FDD995 pKa = 3.71QNTNIYY1001 pKa = 10.15VDD1003 pKa = 3.56VEE1005 pKa = 4.5TTSGHH1010 pKa = 6.83RR1011 pKa = 11.84YY1012 pKa = 9.52LLYY1015 pKa = 10.64TPVDD1019 pKa = 3.66YY1020 pKa = 11.24DD1021 pKa = 3.91GLGTGYY1027 pKa = 8.48TVHH1030 pKa = 6.99HH1031 pKa = 6.58GLGVDD1036 pKa = 3.78AKK1038 pKa = 10.3NGLWHH1043 pKa = 6.67TYY1045 pKa = 10.14RR1046 pKa = 11.84RR1047 pKa = 11.84DD1048 pKa = 3.38LQADD1052 pKa = 3.97LEE1054 pKa = 4.39EE1055 pKa = 4.67GQPGVSILEE1064 pKa = 4.11VNGFLIRR1071 pKa = 11.84GSGSVDD1077 pKa = 4.19DD1078 pKa = 4.75IGLMDD1083 pKa = 4.3TMLINQDD1090 pKa = 3.34TDD1092 pKa = 3.22SDD1094 pKa = 4.81GITDD1098 pKa = 4.04FNEE1101 pKa = 3.76TNIYY1105 pKa = 8.55GTDD1108 pKa = 3.62PNKK1111 pKa = 10.85ADD1113 pKa = 3.74TDD1115 pKa = 3.57EE1116 pKa = 5.97DD1117 pKa = 4.77GIIDD1121 pKa = 4.4GDD1123 pKa = 3.89EE1124 pKa = 3.73LSYY1127 pKa = 10.53WGLNWDD1133 pKa = 3.88EE1134 pKa = 6.03DD1135 pKa = 4.49YY1136 pKa = 11.69DD1137 pKa = 4.42GDD1139 pKa = 4.77NLINLLDD1146 pKa = 4.64PDD1148 pKa = 4.33ADD1150 pKa = 3.61NDD1152 pKa = 3.88GFLDD1156 pKa = 3.97GEE1158 pKa = 4.4EE1159 pKa = 4.12IAAGSDD1165 pKa = 3.4PADD1168 pKa = 4.18PEE1170 pKa = 4.38SSPLSLVVYY1179 pKa = 10.08EE1180 pKa = 4.66DD1181 pKa = 4.9AEE1183 pKa = 4.44DD1184 pKa = 3.64SSIIGWDD1191 pKa = 3.65VYY1193 pKa = 11.73DD1194 pKa = 5.6DD1195 pKa = 4.93DD1196 pKa = 5.49PPGALISNVYY1206 pKa = 10.26DD1207 pKa = 3.48EE1208 pKa = 4.88NRR1210 pKa = 11.84QSRR1213 pKa = 11.84VIEE1216 pKa = 4.11FSGSGVTNGYY1226 pKa = 9.16RR1227 pKa = 11.84LRR1229 pKa = 11.84DD1230 pKa = 3.49QNGAPWHH1237 pKa = 5.28NTRR1240 pKa = 11.84QFVIEE1245 pKa = 4.0WSMKK1249 pKa = 9.71FDD1251 pKa = 3.71QNTNIYY1257 pKa = 10.15VDD1259 pKa = 3.56VEE1261 pKa = 4.5TTSGHH1266 pKa = 6.83RR1267 pKa = 11.84YY1268 pKa = 9.52LLYY1271 pKa = 10.64TPVDD1275 pKa = 3.66YY1276 pKa = 11.24DD1277 pKa = 3.91GLGTGYY1283 pKa = 8.48TVHH1286 pKa = 6.99HH1287 pKa = 6.58GLGVDD1292 pKa = 3.78AKK1294 pKa = 10.3NGLWHH1299 pKa = 6.67TYY1301 pKa = 10.14RR1302 pKa = 11.84RR1303 pKa = 11.84DD1304 pKa = 3.38LQADD1308 pKa = 3.97LEE1310 pKa = 4.39EE1311 pKa = 4.67GQPGVSILEE1320 pKa = 4.11VNGFLIRR1327 pKa = 11.84GSGSLDD1333 pKa = 3.92DD1334 pKa = 5.71IILKK1338 pKa = 9.14EE1339 pKa = 4.09QYY1341 pKa = 10.63

Molecular weight: 149.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6HHA8|A0A2P6HHA8_9DELT Arginine--tRNA ligase (Fragment) OS=Desulfobacteraceae bacterium SEEP-SAG10 OX=2100160 GN=argS PE=3 SV=1
LL1 pKa = 7.12LRR3 pKa = 11.84YY4 pKa = 9.76GDD6 pKa = 4.1RR7 pKa = 11.84VNMAFGVEE15 pKa = 4.11NRR17 pKa = 11.84CPFLDD22 pKa = 3.36HH23 pKa = 7.29RR24 pKa = 11.84LIEE27 pKa = 4.31YY28 pKa = 10.4VSALPSHH35 pKa = 5.97MKK37 pKa = 9.95IRR39 pKa = 11.84GGTTKK44 pKa = 10.08WIFRR48 pKa = 11.84EE49 pKa = 4.18VADD52 pKa = 4.06GRR54 pKa = 11.84IPKK57 pKa = 10.2AILKK61 pKa = 10.02RR62 pKa = 11.84RR63 pKa = 11.84LKK65 pKa = 10.32MGFPTPLGVWLRR77 pKa = 11.84KK78 pKa = 9.02EE79 pKa = 4.03LPEE82 pKa = 5.38VARR85 pKa = 11.84QWLGNYY91 pKa = 9.26RR92 pKa = 11.84FLPFFDD98 pKa = 3.31RR99 pKa = 11.84WII101 pKa = 4.14

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

614

0

614

116105

20

1341

189.1

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.695 ± 0.117

1.273 ± 0.046

5.704 ± 0.114

6.492 ± 0.1

4.621 ± 0.092

6.739 ± 0.13

2.117 ± 0.051

7.935 ± 0.116

7.502 ± 0.144

9.465 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.051

4.39 ± 0.079

4.043 ± 0.061

3.168 ± 0.069

5.296 ± 0.092

6.15 ± 0.083

5.038 ± 0.075

6.324 ± 0.093

1.111 ± 0.051

3.462 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski