Mesonia algae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes;

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2W7HW89|A0A2W7HW89_9FLAO DNA helicase OS=Mesonia algae OX=213248 GN=LX95_02870 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.28KK3 pKa = 8.54TYY5 pKa = 10.72LSILIALISITSFAQEE21 pKa = 3.96ALITGYY27 pKa = 10.66VDD29 pKa = 4.1SPCSGANGRR38 pKa = 11.84VLEE41 pKa = 4.38IYY43 pKa = 11.17VNGTIDD49 pKa = 3.52FTGWNIQRR57 pKa = 11.84QSNGGGYY64 pKa = 7.78STNIDD69 pKa = 3.49LSSFATISDD78 pKa = 3.52DD79 pKa = 5.77FIYY82 pKa = 9.52LTNDD86 pKa = 3.11QAILNAEE93 pKa = 4.19FGITANVIEE102 pKa = 4.62SSVISSNGNDD112 pKa = 3.16AFQIIDD118 pKa = 3.74NNSVVIDD125 pKa = 3.76RR126 pKa = 11.84FGEE129 pKa = 4.1EE130 pKa = 4.37NIDD133 pKa = 3.71GAGTAWEE140 pKa = 4.68HH141 pKa = 6.37LDD143 pKa = 4.52SYY145 pKa = 9.99YY146 pKa = 11.02LRR148 pKa = 11.84NNSSIANAGSFDD160 pKa = 3.79VNNWTFGALNSLDD173 pKa = 3.71NQGLCNSSSALSTMVNLGSFTPVVTTVAEE202 pKa = 4.14IEE204 pKa = 3.94FDD206 pKa = 3.26EE207 pKa = 5.34AYY209 pKa = 9.78ISAVEE214 pKa = 4.22GSGTATLSVTISEE227 pKa = 5.02LPNSDD232 pKa = 3.88ANIDD236 pKa = 3.58VAILVNEE243 pKa = 4.08STAIDD248 pKa = 3.9NQHH251 pKa = 4.75YY252 pKa = 8.7TYY254 pKa = 10.9TNEE257 pKa = 4.0TLTFTTTGSLSQSITITIPDD277 pKa = 3.93NFDD280 pKa = 3.58AEE282 pKa = 4.61ADD284 pKa = 3.68TFLALEE290 pKa = 4.23LTNPVNANLGEE301 pKa = 4.79DD302 pKa = 3.73NLSVVYY308 pKa = 10.56ILDD311 pKa = 4.01NEE313 pKa = 4.26AHH315 pKa = 6.44SPIATQNLDD324 pKa = 3.21INFATSYY331 pKa = 10.88AITGPNPGSEE341 pKa = 3.89IVAHH345 pKa = 6.86DD346 pKa = 3.74ATTEE350 pKa = 3.89RR351 pKa = 11.84LFVMNSGNASVEE363 pKa = 3.91ILDD366 pKa = 4.23FSSPLNITSISTIDD380 pKa = 3.45LSSYY384 pKa = 10.61GDD386 pKa = 3.33SSTSVAVHH394 pKa = 6.38NGLVAATAVPNDD406 pKa = 3.34KK407 pKa = 10.66NLNGKK412 pKa = 7.96VVFMDD417 pKa = 3.52TNGVIITSLEE427 pKa = 4.21VGSLPDD433 pKa = 4.48MITFSPDD440 pKa = 2.53GNMLLVANEE449 pKa = 4.39GEE451 pKa = 4.3PNSDD455 pKa = 3.33YY456 pKa = 11.21TIDD459 pKa = 3.77PEE461 pKa = 4.49GTISIIDD468 pKa = 3.78LSIATANLTQANVTTLNFNAFDD490 pKa = 3.83TQEE493 pKa = 4.21TQLKK497 pKa = 9.62ADD499 pKa = 3.94GVRR502 pKa = 11.84IFGPNATVSQDD513 pKa = 3.74LEE515 pKa = 4.14PEE517 pKa = 4.42YY518 pKa = 10.37ITVSQDD524 pKa = 2.49NTTAWVSLQEE534 pKa = 4.06NNAIAVVDD542 pKa = 4.43LTTMQITNILPLGLKK557 pKa = 10.03DD558 pKa = 4.43HH559 pKa = 6.77SLPNNALDD567 pKa = 3.84TSNEE571 pKa = 3.49QDD573 pKa = 4.31FIFMANWPIKK583 pKa = 9.56GMYY586 pKa = 8.77MPDD589 pKa = 4.13AIASYY594 pKa = 10.4SVNGNTYY601 pKa = 9.88IVTANEE607 pKa = 3.7GDD609 pKa = 3.3ARR611 pKa = 11.84EE612 pKa = 3.91YY613 pKa = 10.67DD614 pKa = 3.6TFEE617 pKa = 4.23EE618 pKa = 4.52EE619 pKa = 4.79VNIEE623 pKa = 4.74DD624 pKa = 3.55IVLDD628 pKa = 3.68ASVFPNQDD636 pKa = 3.06FLEE639 pKa = 4.97LDD641 pKa = 3.7EE642 pKa = 4.99NLGKK646 pKa = 10.31IKK648 pKa = 9.35ITSEE652 pKa = 4.02FGDD655 pKa = 3.45IDD657 pKa = 3.88NDD659 pKa = 4.22GEE661 pKa = 4.13FEE663 pKa = 4.31EE664 pKa = 4.39IHH666 pKa = 6.1VFGGRR671 pKa = 11.84SFSIYY676 pKa = 10.51DD677 pKa = 4.77AITGAQVYY685 pKa = 10.54DD686 pKa = 3.59SGSDD690 pKa = 3.42FEE692 pKa = 5.74RR693 pKa = 11.84IIAEE697 pKa = 4.06DD698 pKa = 3.55PVYY701 pKa = 10.99NAIFNATDD709 pKa = 4.27DD710 pKa = 3.99EE711 pKa = 4.8NEE713 pKa = 4.08LKK715 pKa = 10.75NRR717 pKa = 11.84SDD719 pKa = 3.58NKK721 pKa = 10.23GPEE724 pKa = 3.98PEE726 pKa = 3.86AVIVQEE732 pKa = 4.3IDD734 pKa = 3.06GTFYY738 pKa = 11.49AFIALEE744 pKa = 4.06RR745 pKa = 11.84VGGFMVYY752 pKa = 10.47DD753 pKa = 3.68ITNPNTPVFEE763 pKa = 5.01GYY765 pKa = 10.42FNNRR769 pKa = 11.84SVTPGEE775 pKa = 3.98GDD777 pKa = 3.51LANLGDD783 pKa = 4.7LAPEE787 pKa = 4.38SIVYY791 pKa = 9.66IAPEE795 pKa = 3.79NNAEE799 pKa = 4.1NKK801 pKa = 10.15GLIVIANEE809 pKa = 3.7VSATISVYY817 pKa = 8.27TLEE820 pKa = 4.51NNVLTTEE827 pKa = 4.04SFEE830 pKa = 4.42VNNSFVVYY838 pKa = 8.74PNPATSSRR846 pKa = 11.84VFFAQPTTYY855 pKa = 10.61EE856 pKa = 4.23LYY858 pKa = 10.44DD859 pKa = 3.27IQGRR863 pKa = 11.84KK864 pKa = 7.94VKK866 pKa = 10.17AAQNSTFINVDD877 pKa = 3.19GLNAGTYY884 pKa = 8.76IVKK887 pKa = 10.35NDD889 pKa = 3.33GGQTQKK895 pKa = 11.4LIIKK899 pKa = 9.66

Molecular weight:
97.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2W7JUA2|A0A2W7JUA2_9FLAO Uncharacterized protein OS=Mesonia algae OX=213248 GN=LX95_02170 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight:
6.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2856

0

2856

933224

40

2403

326.8

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.09 ± 0.041

0.71 ± 0.016

5.351 ± 0.041

7.24 ± 0.046

5.262 ± 0.035

6.062 ± 0.045

1.798 ± 0.024

8.009 ± 0.041

8.133 ± 0.074

9.504 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.025

6.241 ± 0.055

3.268 ± 0.022

3.763 ± 0.032

3.244 ± 0.033

6.559 ± 0.042

5.569 ± 0.042

6.029 ± 0.034

0.976 ± 0.018

4.09 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski