Staphylococcus haemolyticus (strain JCSC1435)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus haemolyticus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2640 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4L3P6|Q4L3P6_STAHJ Uncharacterized protein OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=SH2422 PE=4 SV=1
MM1 pKa = 7.44NKK3 pKa = 9.56LAAASQGQNVNDD15 pKa = 5.81LITVSNQYY23 pKa = 9.97IEE25 pKa = 5.01EE26 pKa = 4.34GQNNDD31 pKa = 3.71GIIRR35 pKa = 11.84AHH37 pKa = 6.43EE38 pKa = 4.51DD39 pKa = 3.27EE40 pKa = 5.85PITYY44 pKa = 10.09HH45 pKa = 7.88SDD47 pKa = 2.67ITVDD51 pKa = 3.6DD52 pKa = 4.51AVNSGDD58 pKa = 4.07TMTIDD63 pKa = 3.93YY64 pKa = 9.91DD65 pKa = 3.81SHH67 pKa = 6.94TIPSDD72 pKa = 3.11LTDD75 pKa = 3.32QYY77 pKa = 11.09FVPDD81 pKa = 3.52IADD84 pKa = 3.51SNGDD88 pKa = 3.63IIATGTYY95 pKa = 10.87DD96 pKa = 4.54DD97 pKa = 3.6ATKK100 pKa = 10.53RR101 pKa = 11.84ATYY104 pKa = 9.3TFTVFVDD111 pKa = 3.11THH113 pKa = 7.34SNVTGTLSLSSFIDD127 pKa = 3.42KK128 pKa = 10.92SKK130 pKa = 11.16VPARR134 pKa = 11.84DD135 pKa = 3.31TNIDD139 pKa = 3.6MTFKK143 pKa = 10.11TANTAYY149 pKa = 9.79NQNFTVDD156 pKa = 3.48YY157 pKa = 10.13QYY159 pKa = 11.57PEE161 pKa = 4.11VEE163 pKa = 4.09GDD165 pKa = 3.82SNII168 pKa = 3.84

Molecular weight: 18.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4L366|Q4L366_STAHJ Uncharacterized protein OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=SH2602 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2640

0

2640

757125

11

4354

286.8

32.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.096 ± 0.059

0.625 ± 0.014

5.938 ± 0.064

6.738 ± 0.075

4.481 ± 0.055

6.053 ± 0.054

2.298 ± 0.023

8.572 ± 0.076

7.492 ± 0.075

9.122 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.034

5.797 ± 0.058

3.192 ± 0.05

3.951 ± 0.04

3.605 ± 0.04

6.366 ± 0.186

5.768 ± 0.111

6.561 ± 0.046

0.777 ± 0.018

3.927 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski