Pedobacter sp. Hv1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N8W5S0|A0A0N8W5S0_9SPHI Uncharacterized protein OS=Pedobacter sp. Hv1 OX=1740090 GN=AQF98_07145 PE=4 SV=1
MM1 pKa = 7.4EE2 pKa = 6.09ANDD5 pKa = 3.91ISIQVEE11 pKa = 3.93DD12 pKa = 4.0RR13 pKa = 11.84EE14 pKa = 4.78GNISTLIAPTDD25 pKa = 3.44MGLSLMEE32 pKa = 3.86FLKK35 pKa = 10.93ASEE38 pKa = 3.96YY39 pKa = 10.96DD40 pKa = 3.26ILATCGGMALCATCCVDD57 pKa = 4.23VMEE60 pKa = 5.86GEE62 pKa = 4.52DD63 pKa = 4.54KK64 pKa = 10.34LTEE67 pKa = 4.1MTDD70 pKa = 3.29DD71 pKa = 4.22EE72 pKa = 4.96YY73 pKa = 11.94AMLDD77 pKa = 3.8TLPDD81 pKa = 4.06LLPNSRR87 pKa = 11.84LACQLQLSPAMEE99 pKa = 4.18GLKK102 pKa = 10.43VRR104 pKa = 11.84LHH106 pKa = 7.28AMDD109 pKa = 3.76

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q1F0E2|A0A0Q1F0E2_9SPHI Peptidase M56 BlaR1 OS=Pedobacter sp. Hv1 OX=1740090 GN=AQF98_16805 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 5.02MSPFKK7 pKa = 10.76NKK9 pKa = 9.92KK10 pKa = 9.13LPKK13 pKa = 9.85FLGSFLLNNSFYY25 pKa = 9.97WSIYY29 pKa = 9.02GVPALRR35 pKa = 11.84GLPAHH40 pKa = 6.26RR41 pKa = 11.84TFLSRR46 pKa = 11.84FCSSFLLEE54 pKa = 3.97QARR57 pKa = 11.84KK58 pKa = 9.65SLVLIRR64 pKa = 4.66

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4261

0

4261

1437873

36

2996

337.4

37.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.468 ± 0.038

0.752 ± 0.013

5.084 ± 0.026

5.643 ± 0.039

5.054 ± 0.026

6.488 ± 0.035

1.683 ± 0.016

7.614 ± 0.032

7.746 ± 0.04

9.744 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.017

6.048 ± 0.037

3.577 ± 0.018

3.897 ± 0.027

3.425 ± 0.025

6.139 ± 0.027

5.987 ± 0.047

6.221 ± 0.03

1.081 ± 0.014

4.098 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski