Porcine coronavirus HKU15

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Deltacoronavirus; Buldecovirus; Coronavirus HKU15

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9BQX9|H9BQX9_9NIDO Envelope protein OS=Porcine coronavirus HKU15 OX=1159905 GN=E PE=4 SV=1
MM1 pKa = 7.06QRR3 pKa = 11.84ALLIMTLLCLVRR15 pKa = 11.84AKK17 pKa = 10.61FADD20 pKa = 4.01DD21 pKa = 5.1LLDD24 pKa = 4.37LLTFPGAHH32 pKa = 6.45RR33 pKa = 11.84FLHH36 pKa = 5.95KK37 pKa = 9.15PTRR40 pKa = 11.84NSSSLYY46 pKa = 10.58SRR48 pKa = 11.84ANNNFDD54 pKa = 3.65VGVLPGYY61 pKa = 6.59PTKK64 pKa = 10.74NVNLFSPLTNSTLPINGLHH83 pKa = 7.3RR84 pKa = 11.84SYY86 pKa = 11.15QPLMLNCLTKK96 pKa = 9.63ITNHH100 pKa = 5.33TLSMYY105 pKa = 10.28LLPSEE110 pKa = 4.26IQTYY114 pKa = 9.47SCGGAMVKK122 pKa = 10.35YY123 pKa = 7.78QTHH126 pKa = 6.23DD127 pKa = 3.05AVRR130 pKa = 11.84IILDD134 pKa = 3.88LTATDD139 pKa = 4.67HH140 pKa = 7.17ISVEE144 pKa = 4.49VVGQHH149 pKa = 5.4GEE151 pKa = 3.98NYY153 pKa = 10.14VFVCSEE159 pKa = 3.71QFNYY163 pKa = 7.42TTALHH168 pKa = 6.36NSTFFSLNSEE178 pKa = 4.64LYY180 pKa = 10.4CFTNNTYY187 pKa = 10.84LGILPPDD194 pKa = 3.42LTDD197 pKa = 3.5FTVYY201 pKa = 9.24RR202 pKa = 11.84TGQFYY207 pKa = 11.3ANGYY211 pKa = 9.58LLGTLPITVNYY222 pKa = 8.41VRR224 pKa = 11.84LYY226 pKa = 10.42RR227 pKa = 11.84GHH229 pKa = 7.33LSANSAHH236 pKa = 6.5FALANLTDD244 pKa = 3.79TLITLTNTTISQITYY259 pKa = 9.11CDD261 pKa = 3.41KK262 pKa = 11.34SVVDD266 pKa = 5.56SIACQRR272 pKa = 11.84SSHH275 pKa = 5.05EE276 pKa = 4.23VEE278 pKa = 4.65DD279 pKa = 4.8GFYY282 pKa = 10.54SDD284 pKa = 3.98PKK286 pKa = 8.74SAVRR290 pKa = 11.84ARR292 pKa = 11.84QRR294 pKa = 11.84TIVTLPKK301 pKa = 10.35LPEE304 pKa = 4.28LEE306 pKa = 4.16VVQLNISAHH315 pKa = 5.23MDD317 pKa = 3.32FGEE320 pKa = 4.12ARR322 pKa = 11.84LDD324 pKa = 3.63SVTINGNTSYY334 pKa = 10.9CVTKK338 pKa = 10.17PYY340 pKa = 10.45FRR342 pKa = 11.84LEE344 pKa = 4.22TNFMCTGCTMNLRR357 pKa = 11.84TDD359 pKa = 3.25TCSFDD364 pKa = 3.98LSAVNNGMSFSQFCLSTEE382 pKa = 4.12SGACEE387 pKa = 3.57MKK389 pKa = 10.55IIVTYY394 pKa = 9.15VWNYY398 pKa = 10.41LLRR401 pKa = 11.84QRR403 pKa = 11.84LYY405 pKa = 8.2VTAVEE410 pKa = 4.68GQTHH414 pKa = 6.59TGTTSVHH421 pKa = 5.57ATDD424 pKa = 3.7TSSVITDD431 pKa = 3.13VCTDD435 pKa = 3.17YY436 pKa = 11.11TIYY439 pKa = 10.6GVSGTGIIKK448 pKa = 10.47PSDD451 pKa = 3.71LLLHH455 pKa = 6.34NGIAFTSPTGEE466 pKa = 3.83LYY468 pKa = 10.78AFKK471 pKa = 10.78NITTGKK477 pKa = 7.25TLQVLPCEE485 pKa = 4.59TPSQLIVINNTVVGAITSSNSTEE508 pKa = 3.87NNRR511 pKa = 11.84FTTTIVTPTFFYY523 pKa = 10.02STNDD527 pKa = 3.13TTFNCTKK534 pKa = 9.9PVLSYY539 pKa = 11.4GPISVCSDD547 pKa = 3.07GAIVGTSTLQNTRR560 pKa = 11.84PSIVSLYY567 pKa = 10.25DD568 pKa = 3.3GEE570 pKa = 4.75VEE572 pKa = 4.11IPSAFSLSVQTEE584 pKa = 4.19YY585 pKa = 11.31LQVQAEE591 pKa = 4.22QVIVDD596 pKa = 4.14CPQYY600 pKa = 10.72VCNGNSRR607 pKa = 11.84CLQLLAQYY615 pKa = 9.93TSACSNIEE623 pKa = 3.97AALHH627 pKa = 6.46SSAQLDD633 pKa = 3.48SRR635 pKa = 11.84EE636 pKa = 4.23IINMFKK642 pKa = 10.39TSTQSLQLANITNFKK657 pKa = 10.44GDD659 pKa = 3.79YY660 pKa = 9.12NFSSILTTRR669 pKa = 11.84IGGRR673 pKa = 11.84SAIEE677 pKa = 3.73DD678 pKa = 3.67LLFNKK683 pKa = 9.43VVTSGLGTVDD693 pKa = 3.9QDD695 pKa = 3.86YY696 pKa = 10.73KK697 pKa = 10.88SCSRR701 pKa = 11.84DD702 pKa = 3.16MAIADD707 pKa = 4.39LVCSQYY713 pKa = 11.52YY714 pKa = 9.28NGIMVLPGVVDD725 pKa = 3.77AEE727 pKa = 4.19KK728 pKa = 9.5MAMYY732 pKa = 9.35TGSLTGAMVFGGLTAAAAIPFATAVQARR760 pKa = 11.84LNYY763 pKa = 9.74VALQTNVLQEE773 pKa = 4.03NQKK776 pKa = 9.87ILAEE780 pKa = 4.27SFNQAVGNISLALSSVNDD798 pKa = 5.05AIQQTSEE805 pKa = 4.02ALNTVAIAIKK815 pKa = 10.22KK816 pKa = 8.47IQTVVNQQGEE826 pKa = 4.49ALSHH830 pKa = 5.94LTAQLSNNFQAISTSIQDD848 pKa = 2.88IYY850 pKa = 11.71NRR852 pKa = 11.84LEE854 pKa = 3.88EE855 pKa = 4.34VEE857 pKa = 5.42ANQQVDD863 pKa = 3.52RR864 pKa = 11.84LITGRR869 pKa = 11.84LAALNAYY876 pKa = 7.48VTQLLNQMSQIRR888 pKa = 11.84QSRR891 pKa = 11.84LLAQQKK897 pKa = 10.11INEE900 pKa = 4.5CVKK903 pKa = 10.38SQSSRR908 pKa = 11.84YY909 pKa = 8.35GFCGNGTHH917 pKa = 6.91IFSLTQTAPNGIFFMHH933 pKa = 7.03AVLVPNKK940 pKa = 7.88FTRR943 pKa = 11.84VNASAGICVDD953 pKa = 3.12NTRR956 pKa = 11.84GYY958 pKa = 10.79SLQPQLILYY967 pKa = 7.6QFNNSWRR974 pKa = 11.84VTPRR978 pKa = 11.84NMYY981 pKa = 9.92EE982 pKa = 3.71PRR984 pKa = 11.84LPRR987 pKa = 11.84QADD990 pKa = 4.83FIQLTDD996 pKa = 3.91CSVTFYY1002 pKa = 10.62NTTAANLPNIIPDD1015 pKa = 4.05IIDD1018 pKa = 3.39VNQTVSDD1025 pKa = 4.51IIDD1028 pKa = 4.04NLPTATPPQWDD1039 pKa = 3.32VGIYY1043 pKa = 10.91NNTILNLTVEE1053 pKa = 4.37INDD1056 pKa = 3.43LQEE1059 pKa = 3.91RR1060 pKa = 11.84SKK1062 pKa = 11.31NLSQIADD1069 pKa = 3.93RR1070 pKa = 11.84LQNYY1074 pKa = 9.14IDD1076 pKa = 3.98NLNNTLVDD1084 pKa = 4.3LEE1086 pKa = 4.17WLNRR1090 pKa = 11.84VEE1092 pKa = 5.99TYY1094 pKa = 10.72LKK1096 pKa = 8.97WPWYY1100 pKa = 8.19IWLAIALALIAFVTILITIFLCTGCCGGCFGCCGGCFGLFSKK1142 pKa = 10.53KK1143 pKa = 9.65KK1144 pKa = 10.16RR1145 pKa = 11.84YY1146 pKa = 8.31TDD1148 pKa = 3.81DD1149 pKa = 3.27QPTPSFKK1156 pKa = 10.66FKK1158 pKa = 10.4EE1159 pKa = 4.19WW1160 pKa = 3.21

Molecular weight: 128.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060A825|A0A060A825_9NIDO Spike glycoprotein OS=Porcine coronavirus HKU15 OX=1159905 GN=S PE=4 SV=1
MM1 pKa = 7.64AAPVVPTTDD10 pKa = 3.28ASWFQVLKK18 pKa = 10.58AQNKK22 pKa = 9.03KK23 pKa = 8.6ATHH26 pKa = 5.19PQFRR30 pKa = 11.84GNGVPLNSAIKK41 pKa = 9.29PVEE44 pKa = 3.72NHH46 pKa = 6.56GYY48 pKa = 7.02WLRR51 pKa = 11.84YY52 pKa = 7.01TRR54 pKa = 11.84QKK56 pKa = 10.75PGGTPIPPSYY66 pKa = 10.88AFYY69 pKa = 9.95YY70 pKa = 9.86TGTGPRR76 pKa = 11.84GNLKK80 pKa = 10.6YY81 pKa = 11.01GEE83 pKa = 4.96LPPNDD88 pKa = 3.78TPATTRR94 pKa = 11.84VTWVKK99 pKa = 10.94GSGADD104 pKa = 3.53TSIKK108 pKa = 9.78PHH110 pKa = 4.45VAKK113 pKa = 10.68RR114 pKa = 11.84NPNNPKK120 pKa = 9.85HH121 pKa = 5.96QLLPLRR127 pKa = 11.84FPTGDD132 pKa = 3.2GPAQGFRR139 pKa = 11.84VDD141 pKa = 3.64PFNARR146 pKa = 11.84GRR148 pKa = 11.84PQEE151 pKa = 4.34RR152 pKa = 11.84GSGPRR157 pKa = 11.84SQSVNSRR164 pKa = 11.84GTGNQPRR171 pKa = 11.84KK172 pKa = 9.37RR173 pKa = 11.84DD174 pKa = 3.44QSAPAAVRR182 pKa = 11.84RR183 pKa = 11.84KK184 pKa = 7.09TQHH187 pKa = 5.18QAPKK191 pKa = 9.09RR192 pKa = 11.84TLPKK196 pKa = 10.35GKK198 pKa = 8.69TISQVFGNRR207 pKa = 11.84SRR209 pKa = 11.84TGANVGSADD218 pKa = 3.79TEE220 pKa = 4.29KK221 pKa = 10.47TGMADD226 pKa = 3.45PRR228 pKa = 11.84IMALARR234 pKa = 11.84HH235 pKa = 5.28VPGVQEE241 pKa = 3.98MLFAGHH247 pKa = 7.25LEE249 pKa = 4.47SNFQAGAITLTFSYY263 pKa = 10.44SITVKK268 pKa = 10.44EE269 pKa = 4.26GSPDD273 pKa = 3.46YY274 pKa = 11.25DD275 pKa = 3.37RR276 pKa = 11.84LKK278 pKa = 10.99DD279 pKa = 3.64ALNTVVNQTYY289 pKa = 9.4EE290 pKa = 4.24PPTKK294 pKa = 8.96PTKK297 pKa = 10.21DD298 pKa = 3.46KK299 pKa = 11.42KK300 pKa = 10.17PDD302 pKa = 3.57KK303 pKa = 10.07QDD305 pKa = 3.05QSAKK309 pKa = 10.09PKK311 pKa = 8.42QQKK314 pKa = 9.59KK315 pKa = 8.18PKK317 pKa = 9.94KK318 pKa = 8.31VTLPADD324 pKa = 3.66KK325 pKa = 10.57QDD327 pKa = 3.86WEE329 pKa = 4.04WDD331 pKa = 3.38DD332 pKa = 3.93AFEE335 pKa = 4.83IKK337 pKa = 10.2QEE339 pKa = 4.19SAAA342 pKa = 4.0

Molecular weight: 37.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

8363

83

6267

1194.7

133.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.701 ± 0.453

2.487 ± 0.425

5.285 ± 0.556

4.054 ± 0.292

4.341 ± 0.429

4.938 ± 0.511

2.344 ± 0.305

5.991 ± 0.676

4.938 ± 0.761

9.59 ± 1.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.316

5.536 ± 0.617

5.034 ± 1.232

4.4 ± 0.46

3.802 ± 0.703

6.11 ± 0.589

7.988 ± 0.869

8.095 ± 0.596

0.849 ± 0.273

4.699 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski