Clostridium sp. KNHs216

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3742 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542AGJ9|A0A542AGJ9_9CLOT Flagellar protein FliS OS=Clostridium sp. KNHs216 OX=1550235 GN=LY85_0102 PE=3 SV=1
MM1 pKa = 8.07AINQINAYY9 pKa = 10.18SYY11 pKa = 8.44GTNSTSGTSSASGTSQTSASKK32 pKa = 10.39NSSVLGLNDD41 pKa = 4.06FLQLLAAQFANQDD54 pKa = 3.47VMNPTDD60 pKa = 3.39NTEE63 pKa = 4.58YY64 pKa = 10.08ISQMAQFSALQAMQEE79 pKa = 4.2LSQISYY85 pKa = 7.61TQYY88 pKa = 10.64GASLVGKK95 pKa = 8.92KK96 pKa = 8.72VVVASYY102 pKa = 7.93DD103 pKa = 3.65TNGKK107 pKa = 7.84YY108 pKa = 9.15QEE110 pKa = 4.27DD111 pKa = 3.84TGIVDD116 pKa = 3.85SVNFATGSNVLVVNGKK132 pKa = 10.21AYY134 pKa = 10.47DD135 pKa = 3.29ISSVMKK141 pKa = 10.34VVNPDD146 pKa = 2.87AATPPDD152 pKa = 4.44EE153 pKa = 4.99TPDD156 pKa = 4.95DD157 pKa = 4.91PDD159 pKa = 6.15DD160 pKa = 5.36PDD162 pKa = 4.01TPDD165 pKa = 3.24VPDD168 pKa = 3.48TGGEE172 pKa = 4.4SVV174 pKa = 3.55

Molecular weight: 18.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542AGI7|A0A542AGI7_9CLOT Translational regulator CsrA OS=Clostridium sp. KNHs216 OX=1550235 GN=csrA PE=3 SV=1
MM1 pKa = 7.43ICAPCAKK8 pKa = 9.76CKK10 pKa = 10.21RR11 pKa = 11.84RR12 pKa = 11.84ADD14 pKa = 3.4NRR16 pKa = 11.84ICRR19 pKa = 11.84RR20 pKa = 11.84FCSAYY25 pKa = 9.52KK26 pKa = 10.21RR27 pKa = 11.84AFEE30 pKa = 4.49RR31 pKa = 11.84NVEE34 pKa = 4.09RR35 pKa = 11.84QLDD38 pKa = 3.67EE39 pKa = 4.05TLFRR43 pKa = 11.84RR44 pKa = 11.84LARR47 pKa = 11.84ISKK50 pKa = 10.09

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3742

0

3742

1147581

37

2462

306.7

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.664 ± 0.043

1.565 ± 0.018

5.4 ± 0.03

6.515 ± 0.04

4.067 ± 0.027

7.628 ± 0.046

1.667 ± 0.015

6.951 ± 0.035

6.068 ± 0.033

9.256 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.023

4.101 ± 0.025

3.815 ± 0.024

3.347 ± 0.023

4.757 ± 0.04

6.378 ± 0.033

5.539 ± 0.041

7.131 ± 0.033

0.817 ± 0.013

3.478 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski