Gordonia phage MelBins

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VN15|A0A649VN15_9CAUD Crossover junction endodeoxyribonuclease RuvC OS=Gordonia phage MelBins OX=2656540 GN=67 PE=3 SV=1
MM1 pKa = 7.6IATDD5 pKa = 4.13ADD7 pKa = 3.71AALADD12 pKa = 3.9YY13 pKa = 7.68WTAKK17 pKa = 10.82ANGDD21 pKa = 3.54EE22 pKa = 4.39DD23 pKa = 4.28AADD26 pKa = 4.14AAHH29 pKa = 7.06LAYY32 pKa = 10.52VNAMYY37 pKa = 7.58ATPP40 pKa = 4.11

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VMX5|A0A649VMX5_9CAUD Uncharacterized protein OS=Gordonia phage MelBins OX=2656540 GN=27 PE=4 SV=1
MM1 pKa = 7.76IPRR4 pKa = 11.84DD5 pKa = 4.04QQPMPTPAEE14 pKa = 4.16AIEE17 pKa = 4.48AYY19 pKa = 9.78KK20 pKa = 10.48AGQVSRR26 pKa = 11.84PGAGNPYY33 pKa = 9.91AGRR36 pKa = 11.84RR37 pKa = 11.84VLGSVWALGNRR48 pKa = 11.84EE49 pKa = 4.17AQRR52 pKa = 11.84VAYY55 pKa = 9.34TEE57 pKa = 3.77MRR59 pKa = 11.84KK60 pKa = 9.54RR61 pKa = 11.84EE62 pKa = 3.95AARR65 pKa = 11.84RR66 pKa = 11.84EE67 pKa = 4.05

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

19240

32

1630

216.2

23.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.625 ± 0.524

1.008 ± 0.15

7.568 ± 0.386

5.447 ± 0.329

2.313 ± 0.222

8.165 ± 0.475

2.256 ± 0.199

4.241 ± 0.179

2.526 ± 0.194

7.708 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.12

2.76 ± 0.152

6.398 ± 0.264

3.763 ± 0.139

7.536 ± 0.382

4.501 ± 0.236

6.907 ± 0.285

7.677 ± 0.192

2.131 ± 0.138

2.152 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski