Vibrio phage phiV208

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0X4U7|A0A6H0X4U7_9CAUD Phage_Mu_F domain-containing protein OS=Vibrio phage phiV208 OX=2723906 GN=phiV208_10 PE=4 SV=1
MM1 pKa = 7.23FLAWCDD7 pKa = 3.34KK8 pKa = 11.01CGDD11 pKa = 4.4AIYY14 pKa = 10.83SDD16 pKa = 4.42DD17 pKa = 4.22EE18 pKa = 4.4IVRR21 pKa = 11.84KK22 pKa = 10.11DD23 pKa = 3.69HH24 pKa = 6.57MNMHH28 pKa = 6.78LEE30 pKa = 4.05CSEE33 pKa = 4.25LNEE36 pKa = 4.11EE37 pKa = 4.58TFGGDD42 pKa = 4.11CDD44 pKa = 3.95GTYY47 pKa = 11.08DD48 pKa = 4.81DD49 pKa = 6.04AMM51 pKa = 6.04

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0X4Z2|A0A6H0X4Z2_9CAUD Uncharacterized protein OS=Vibrio phage phiV208 OX=2723906 GN=phiV208_56 PE=4 SV=1
MM1 pKa = 7.42SNSKK5 pKa = 10.2RR6 pKa = 11.84KK7 pKa = 9.11CAYY10 pKa = 9.04CKK12 pKa = 9.4EE13 pKa = 4.15RR14 pKa = 11.84KK15 pKa = 9.41KK16 pKa = 11.05VEE18 pKa = 3.81TMFIRR23 pKa = 11.84GAQAFCDD30 pKa = 3.48KK31 pKa = 10.24EE32 pKa = 4.63HH33 pKa = 6.71YY34 pKa = 9.9IEE36 pKa = 4.2YY37 pKa = 10.28QVASKK42 pKa = 10.99DD43 pKa = 3.55RR44 pKa = 11.84LVSKK48 pKa = 10.22GRR50 pKa = 11.84KK51 pKa = 6.55IQRR54 pKa = 11.84TEE56 pKa = 3.66LNKK59 pKa = 10.34RR60 pKa = 11.84KK61 pKa = 9.63QKK63 pKa = 10.51LKK65 pKa = 10.74SINEE69 pKa = 4.26YY70 pKa = 10.85ASEE73 pKa = 4.08AQSAVNSYY81 pKa = 9.71IRR83 pKa = 11.84ARR85 pKa = 11.84DD86 pKa = 3.5HH87 pKa = 6.89KK88 pKa = 10.23KK89 pKa = 9.59PCISCGCDD97 pKa = 2.65KK98 pKa = 11.04VSVYY102 pKa = 10.68GGYY105 pKa = 10.03RR106 pKa = 11.84GAGGWDD112 pKa = 2.94AGHH115 pKa = 5.83YY116 pKa = 9.18RR117 pKa = 11.84SRR119 pKa = 11.84GAAAHH124 pKa = 6.51LRR126 pKa = 11.84FNLLNIHH133 pKa = 5.61KK134 pKa = 10.33QCVKK138 pKa = 10.5CNRR141 pKa = 11.84DD142 pKa = 2.82KK143 pKa = 11.4SGNVVDD149 pKa = 4.67YY150 pKa = 10.55RR151 pKa = 11.84INLIKK156 pKa = 10.59RR157 pKa = 11.84IGVDD161 pKa = 3.5LVEE164 pKa = 5.05KK165 pKa = 10.78LEE167 pKa = 4.64SDD169 pKa = 3.89NEE171 pKa = 4.17PKK173 pKa = 10.77KK174 pKa = 10.93FDD176 pKa = 3.21VDD178 pKa = 3.39YY179 pKa = 10.96LNRR182 pKa = 11.84VKK184 pKa = 10.68RR185 pKa = 11.84IFNKK189 pKa = 9.69RR190 pKa = 11.84SRR192 pKa = 11.84WYY194 pKa = 9.93KK195 pKa = 9.77KK196 pKa = 9.81RR197 pKa = 11.84RR198 pKa = 11.84GMM200 pKa = 4.54

Molecular weight: 23.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13974

35

1176

183.9

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.6 ± 0.477

1.224 ± 0.144

6.462 ± 0.237

7.664 ± 0.487

3.528 ± 0.152

6.899 ± 0.284

1.717 ± 0.147

6.348 ± 0.185

7.027 ± 0.411

7.249 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.202

5.253 ± 0.22

3.492 ± 0.219

4.236 ± 0.299

4.673 ± 0.251

6.777 ± 0.264

5.51 ± 0.365

6.247 ± 0.318

1.553 ± 0.119

3.685 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski