Moelleriella libera RCEF 2490

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Moelleriella; Moelleriella libera

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A162I661|A0A162I661_9HYPO Uncharacterized protein OS=Moelleriella libera RCEF 2490 OX=1081109 GN=AAL_07781 PE=4 SV=1
MM1 pKa = 7.54SFAQTSTEE9 pKa = 3.58YY10 pKa = 10.1WLEE13 pKa = 4.44DD14 pKa = 3.12GHH16 pKa = 8.23ILHH19 pKa = 7.54AYY21 pKa = 7.95CQNADD26 pKa = 3.45GDD28 pKa = 4.77SVQSQIDD35 pKa = 3.77LNGFIGNSDD44 pKa = 3.31GWFEE48 pKa = 3.94WGGVDD53 pKa = 4.45FTEE56 pKa = 4.3TAEE59 pKa = 4.2NIQLEE64 pKa = 4.64GSRR67 pKa = 11.84LTAEE71 pKa = 5.04LYY73 pKa = 9.54TVEE76 pKa = 4.18GGTRR80 pKa = 11.84EE81 pKa = 3.96RR82 pKa = 11.84QGIEE86 pKa = 3.59LNDD89 pKa = 4.64RR90 pKa = 11.84IGNDD94 pKa = 3.45NGQLSYY100 pKa = 10.92IGGG103 pKa = 3.7

Molecular weight:
11.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A168F3T7|A0A168F3T7_9HYPO Uncharacterized protein OS=Moelleriella libera RCEF 2490 OX=1081109 GN=AAL_02015 PE=4 SV=1
MM1 pKa = 7.22VNHH4 pKa = 6.78APARR8 pKa = 11.84RR9 pKa = 11.84PRR11 pKa = 11.84ISAPLGPVKK20 pKa = 10.53SSRR23 pKa = 11.84GPDD26 pKa = 3.19LCRR29 pKa = 11.84SQDD32 pKa = 3.27LRR34 pKa = 11.84VIRR37 pKa = 11.84SIDD40 pKa = 3.5DD41 pKa = 3.44CQIGYY46 pKa = 9.53DD47 pKa = 3.59AAAAAAAANMDD58 pKa = 4.31PGSGAGATLTTSATTATLSSIPIASKK84 pKa = 9.21TRR86 pKa = 11.84FRR88 pKa = 11.84RR89 pKa = 11.84LSASFLSSQPPPPSTPAVAADD110 pKa = 3.85SFIHH114 pKa = 6.85AGPKK118 pKa = 8.49TCFGTMPSKK127 pKa = 9.72TARR130 pKa = 11.84SALTSSCSSKK140 pKa = 11.05SFLRR144 pKa = 11.84PAVARR149 pKa = 11.84YY150 pKa = 9.38AFAPASSASASASASASSSSFSAYY174 pKa = 9.67RR175 pKa = 11.84DD176 pKa = 3.71SPP178 pKa = 3.73

Molecular weight:
18.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8458

0

8458

4278114

49

8775

505.8

55.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.387 ± 0.024

1.224 ± 0.01

5.927 ± 0.019

5.91 ± 0.026

3.595 ± 0.015

7.07 ± 0.027

2.466 ± 0.013

4.481 ± 0.019

4.712 ± 0.026

8.823 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.009

3.429 ± 0.015

5.965 ± 0.027

4.172 ± 0.023

6.528 ± 0.022

8.347 ± 0.031

5.713 ± 0.021

6.202 ± 0.022

1.399 ± 0.01

2.471 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski