Flaviramulus basaltis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flaviramulus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K2IHV2|A0A1K2IHV2_9FLAO Solute:Na+ symporter SSS family OS=Flaviramulus basaltis OX=369401 GN=SAMN05428642_102475 PE=3 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.88YY4 pKa = 11.26YY5 pKa = 10.72LILLALLITIMSYY18 pKa = 11.09AQMGVGTTFPDD29 pKa = 3.46EE30 pKa = 4.24SAQLDD35 pKa = 3.95VVSNDD40 pKa = 2.98KK41 pKa = 11.2GILIPRR47 pKa = 11.84VTLQNSTDD55 pKa = 3.32TTTISSDD62 pKa = 3.44LLSNPISLLVYY73 pKa = 8.31NTKK76 pKa = 10.77ASGDD80 pKa = 4.0LIEE83 pKa = 6.32GYY85 pKa = 10.38HH86 pKa = 5.56YY87 pKa = 10.82WNGSKK92 pKa = 9.41WLRR95 pKa = 11.84LINSDD100 pKa = 3.89DD101 pKa = 3.99SNGVVTTLVDD111 pKa = 3.73NNDD114 pKa = 3.05GTFTYY119 pKa = 10.07TSEE122 pKa = 4.71NNTQTTFDD130 pKa = 4.34ADD132 pKa = 3.69GDD134 pKa = 4.58LIDD137 pKa = 4.72NNNGTFTYY145 pKa = 10.05TNAANTVTTFDD156 pKa = 3.64AKK158 pKa = 9.84LTSVIDD164 pKa = 3.98NSDD167 pKa = 3.08GTYY170 pKa = 10.33TITDD174 pKa = 3.68DD175 pKa = 3.86FGISITIGGATEE187 pKa = 4.16TTTTLVDD194 pKa = 3.72NNDD197 pKa = 3.05GTFTYY202 pKa = 10.04TSEE205 pKa = 5.71DD206 pKa = 3.39NTQTTLTSGSLTNNGDD222 pKa = 3.09GSYY225 pKa = 9.32TFTDD229 pKa = 3.04ATGINTTILASTGLAIEE246 pKa = 4.4PWNGVDD252 pKa = 5.09DD253 pKa = 5.02NGPATDD259 pKa = 3.83NTEE262 pKa = 4.59DD263 pKa = 3.94IYY265 pKa = 11.42TLGDD269 pKa = 3.29VGIGTNTPSATLEE282 pKa = 4.03VNGNVIIGNGGTAIRR297 pKa = 11.84RR298 pKa = 11.84SLSTTAVLDD307 pKa = 4.04FPDD310 pKa = 3.57RR311 pKa = 11.84RR312 pKa = 11.84VINQPEE318 pKa = 4.23LTITLAGANIGDD330 pKa = 4.25VLCLGVPPAAMITFAYY346 pKa = 9.05YY347 pKa = 9.83IAWVSSPNVITIKK360 pKa = 10.28QRR362 pKa = 11.84NSSYY366 pKa = 11.2NPSDD370 pKa = 3.32SDD372 pKa = 3.75APPATFRR379 pKa = 11.84VTVFQYY385 pKa = 11.32

Molecular weight: 41.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K2IJE8|A0A1K2IJE8_9FLAO PAS domain S-box-containing protein OS=Flaviramulus basaltis OX=369401 GN=SAMN05428642_102673 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.92TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3670

0

3670

1276762

28

4418

347.9

39.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.895 ± 0.045

0.745 ± 0.013

5.698 ± 0.033

6.482 ± 0.04

5.301 ± 0.031

6.236 ± 0.043

1.696 ± 0.021

8.45 ± 0.038

8.081 ± 0.065

9.216 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.024

6.901 ± 0.046

3.243 ± 0.022

3.138 ± 0.023

3.06 ± 0.028

6.701 ± 0.034

5.883 ± 0.056

5.96 ± 0.028

1.055 ± 0.015

4.192 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski